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Next‐generation transgenic cotton: pyramiding RNAi and Bt counters insect resistance

Transgenic crops producing insecticidal proteins from the bacterium Bacillus thuringiensis (Bt) are extensively cultivated worldwide. To counter rapidly increasing pest resistance to crops that produce single Bt toxins, transgenic plant ‘pyramids’ producing two or more Bt toxins that kill the same p...

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Autores principales: Ni, Mi, Ma, Wei, Wang, Xiaofang, Gao, Meijing, Dai, Yan, Wei, Xiaoli, Zhang, Lei, Peng, Yonggang, Chen, Shuyuan, Ding, Lingyun, Tian, Yue, Li, Jie, Wang, Haiping, Wang, Xiaolin, Xu, Guowang, Guo, Wangzhen, Yang, Yihua, Wu, Yidong, Heuberger, Shannon, Tabashnik, Bruce E., Zhang, Tianzhen, Zhu, Zhen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5552478/
https://www.ncbi.nlm.nih.gov/pubmed/28199783
http://dx.doi.org/10.1111/pbi.12709
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author Ni, Mi
Ma, Wei
Wang, Xiaofang
Gao, Meijing
Dai, Yan
Wei, Xiaoli
Zhang, Lei
Peng, Yonggang
Chen, Shuyuan
Ding, Lingyun
Tian, Yue
Li, Jie
Wang, Haiping
Wang, Xiaolin
Xu, Guowang
Guo, Wangzhen
Yang, Yihua
Wu, Yidong
Heuberger, Shannon
Tabashnik, Bruce E.
Zhang, Tianzhen
Zhu, Zhen
author_facet Ni, Mi
Ma, Wei
Wang, Xiaofang
Gao, Meijing
Dai, Yan
Wei, Xiaoli
Zhang, Lei
Peng, Yonggang
Chen, Shuyuan
Ding, Lingyun
Tian, Yue
Li, Jie
Wang, Haiping
Wang, Xiaolin
Xu, Guowang
Guo, Wangzhen
Yang, Yihua
Wu, Yidong
Heuberger, Shannon
Tabashnik, Bruce E.
Zhang, Tianzhen
Zhu, Zhen
author_sort Ni, Mi
collection PubMed
description Transgenic crops producing insecticidal proteins from the bacterium Bacillus thuringiensis (Bt) are extensively cultivated worldwide. To counter rapidly increasing pest resistance to crops that produce single Bt toxins, transgenic plant ‘pyramids’ producing two or more Bt toxins that kill the same pest have been widely adopted. However, cross‐resistance and antagonism between Bt toxins limit the sustainability of this approach. Here we describe development and testing of the first pyramids of cotton combining protection from a Bt toxin and RNA interference (RNAi). We developed two types of transgenic cotton plants producing double‐stranded RNA (dsRNA) from the global lepidopteran pest Helicoverpa armigera designed to interfere with its metabolism of juvenile hormone (JH). We focused on suppression of JH acid methyltransferase (JHAMT), which is crucial for JH synthesis, and JH‐binding protein (JHBP), which transports JH to organs. In 2015 and 2016, we tested larvae from a Bt‐resistant strain and a related susceptible strain of H. armigera on seven types of cotton: two controls, Bt cotton, two types of RNAi cotton (targeting JHAMT or JHBP) and two pyramids (Bt cotton plus each type of RNAi). Both types of RNAi cotton were effective against Bt‐resistant insects. Bt cotton and RNAi acted independently against the susceptible strain. In computer simulations of conditions in northern China, where millions of farmers grow Bt cotton as well as abundant non‐transgenic host plants of H. armigera, pyramided cotton combining a Bt toxin and RNAi substantially delayed resistance relative to using Bt cotton alone.
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spelling pubmed-55524782017-08-25 Next‐generation transgenic cotton: pyramiding RNAi and Bt counters insect resistance Ni, Mi Ma, Wei Wang, Xiaofang Gao, Meijing Dai, Yan Wei, Xiaoli Zhang, Lei Peng, Yonggang Chen, Shuyuan Ding, Lingyun Tian, Yue Li, Jie Wang, Haiping Wang, Xiaolin Xu, Guowang Guo, Wangzhen Yang, Yihua Wu, Yidong Heuberger, Shannon Tabashnik, Bruce E. Zhang, Tianzhen Zhu, Zhen Plant Biotechnol J Research Articles Transgenic crops producing insecticidal proteins from the bacterium Bacillus thuringiensis (Bt) are extensively cultivated worldwide. To counter rapidly increasing pest resistance to crops that produce single Bt toxins, transgenic plant ‘pyramids’ producing two or more Bt toxins that kill the same pest have been widely adopted. However, cross‐resistance and antagonism between Bt toxins limit the sustainability of this approach. Here we describe development and testing of the first pyramids of cotton combining protection from a Bt toxin and RNA interference (RNAi). We developed two types of transgenic cotton plants producing double‐stranded RNA (dsRNA) from the global lepidopteran pest Helicoverpa armigera designed to interfere with its metabolism of juvenile hormone (JH). We focused on suppression of JH acid methyltransferase (JHAMT), which is crucial for JH synthesis, and JH‐binding protein (JHBP), which transports JH to organs. In 2015 and 2016, we tested larvae from a Bt‐resistant strain and a related susceptible strain of H. armigera on seven types of cotton: two controls, Bt cotton, two types of RNAi cotton (targeting JHAMT or JHBP) and two pyramids (Bt cotton plus each type of RNAi). Both types of RNAi cotton were effective against Bt‐resistant insects. Bt cotton and RNAi acted independently against the susceptible strain. In computer simulations of conditions in northern China, where millions of farmers grow Bt cotton as well as abundant non‐transgenic host plants of H. armigera, pyramided cotton combining a Bt toxin and RNAi substantially delayed resistance relative to using Bt cotton alone. John Wiley and Sons Inc. 2017-03-16 2017-09 /pmc/articles/PMC5552478/ /pubmed/28199783 http://dx.doi.org/10.1111/pbi.12709 Text en © 2017 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Ni, Mi
Ma, Wei
Wang, Xiaofang
Gao, Meijing
Dai, Yan
Wei, Xiaoli
Zhang, Lei
Peng, Yonggang
Chen, Shuyuan
Ding, Lingyun
Tian, Yue
Li, Jie
Wang, Haiping
Wang, Xiaolin
Xu, Guowang
Guo, Wangzhen
Yang, Yihua
Wu, Yidong
Heuberger, Shannon
Tabashnik, Bruce E.
Zhang, Tianzhen
Zhu, Zhen
Next‐generation transgenic cotton: pyramiding RNAi and Bt counters insect resistance
title Next‐generation transgenic cotton: pyramiding RNAi and Bt counters insect resistance
title_full Next‐generation transgenic cotton: pyramiding RNAi and Bt counters insect resistance
title_fullStr Next‐generation transgenic cotton: pyramiding RNAi and Bt counters insect resistance
title_full_unstemmed Next‐generation transgenic cotton: pyramiding RNAi and Bt counters insect resistance
title_short Next‐generation transgenic cotton: pyramiding RNAi and Bt counters insect resistance
title_sort next‐generation transgenic cotton: pyramiding rnai and bt counters insect resistance
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5552478/
https://www.ncbi.nlm.nih.gov/pubmed/28199783
http://dx.doi.org/10.1111/pbi.12709
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