Cargando…

Synthesis of bacteriophage lytic proteins against Streptococcus pneumoniae in the chloroplast of Chlamydomonas reinhardtii

There is a pressing need to develop novel antibacterial agents given the widespread antibiotic resistance among pathogenic bacteria and the low specificity of the drugs available. Endolysins are antibacterial proteins that are produced by bacteriophage‐infected cells to digest the bacterial cell wal...

Descripción completa

Detalles Bibliográficos
Autores principales: Stoffels, Laura, Taunt, Henry N., Charalambous, Bambos, Purton, Saul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5552482/
https://www.ncbi.nlm.nih.gov/pubmed/28160380
http://dx.doi.org/10.1111/pbi.12703
_version_ 1783256476146466816
author Stoffels, Laura
Taunt, Henry N.
Charalambous, Bambos
Purton, Saul
author_facet Stoffels, Laura
Taunt, Henry N.
Charalambous, Bambos
Purton, Saul
author_sort Stoffels, Laura
collection PubMed
description There is a pressing need to develop novel antibacterial agents given the widespread antibiotic resistance among pathogenic bacteria and the low specificity of the drugs available. Endolysins are antibacterial proteins that are produced by bacteriophage‐infected cells to digest the bacterial cell wall for phage progeny release at the end of the lytic cycle. These highly efficient enzymes show a considerable degree of specificity for the target bacterium of the phage. Furthermore, the emergence of resistance against endolysins appears to be rare as the enzymes have evolved to target molecules in the cell wall that are essential for bacterial viability. Taken together, these factors make recombinant endolysins promising novel antibacterial agents. The chloroplast of the green unicellular alga Chlamydomonas reinhardtii represents an attractive platform for production of therapeutic proteins in general, not least due to the availability of established techniques for foreign gene expression, a lack of endotoxins or potentially infectious agents in the algal host, and low cost of cultivation. The chloroplast is particularly well suited to the production of endolysins as it mimics the native bacterial expression environment of these proteins while being devoid of their cell wall target. In this study, the endolysins Cpl‐1 and Pal, specific to the major human pathogen Streptococcus pneumoniae, were produced in the C. reinhardtii chloroplast. The antibacterial activity of cell lysates and the isolated endolysins was demonstrated against different serotypes of S. pneumoniae, including clinical isolates and total recombinant protein yield was quantified at ~1.3 mg/g algal dry weight.
format Online
Article
Text
id pubmed-5552482
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-55524822017-08-25 Synthesis of bacteriophage lytic proteins against Streptococcus pneumoniae in the chloroplast of Chlamydomonas reinhardtii Stoffels, Laura Taunt, Henry N. Charalambous, Bambos Purton, Saul Plant Biotechnol J Research Articles There is a pressing need to develop novel antibacterial agents given the widespread antibiotic resistance among pathogenic bacteria and the low specificity of the drugs available. Endolysins are antibacterial proteins that are produced by bacteriophage‐infected cells to digest the bacterial cell wall for phage progeny release at the end of the lytic cycle. These highly efficient enzymes show a considerable degree of specificity for the target bacterium of the phage. Furthermore, the emergence of resistance against endolysins appears to be rare as the enzymes have evolved to target molecules in the cell wall that are essential for bacterial viability. Taken together, these factors make recombinant endolysins promising novel antibacterial agents. The chloroplast of the green unicellular alga Chlamydomonas reinhardtii represents an attractive platform for production of therapeutic proteins in general, not least due to the availability of established techniques for foreign gene expression, a lack of endotoxins or potentially infectious agents in the algal host, and low cost of cultivation. The chloroplast is particularly well suited to the production of endolysins as it mimics the native bacterial expression environment of these proteins while being devoid of their cell wall target. In this study, the endolysins Cpl‐1 and Pal, specific to the major human pathogen Streptococcus pneumoniae, were produced in the C. reinhardtii chloroplast. The antibacterial activity of cell lysates and the isolated endolysins was demonstrated against different serotypes of S. pneumoniae, including clinical isolates and total recombinant protein yield was quantified at ~1.3 mg/g algal dry weight. John Wiley and Sons Inc. 2017-03-07 2017-09 /pmc/articles/PMC5552482/ /pubmed/28160380 http://dx.doi.org/10.1111/pbi.12703 Text en © 2017 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Stoffels, Laura
Taunt, Henry N.
Charalambous, Bambos
Purton, Saul
Synthesis of bacteriophage lytic proteins against Streptococcus pneumoniae in the chloroplast of Chlamydomonas reinhardtii
title Synthesis of bacteriophage lytic proteins against Streptococcus pneumoniae in the chloroplast of Chlamydomonas reinhardtii
title_full Synthesis of bacteriophage lytic proteins against Streptococcus pneumoniae in the chloroplast of Chlamydomonas reinhardtii
title_fullStr Synthesis of bacteriophage lytic proteins against Streptococcus pneumoniae in the chloroplast of Chlamydomonas reinhardtii
title_full_unstemmed Synthesis of bacteriophage lytic proteins against Streptococcus pneumoniae in the chloroplast of Chlamydomonas reinhardtii
title_short Synthesis of bacteriophage lytic proteins against Streptococcus pneumoniae in the chloroplast of Chlamydomonas reinhardtii
title_sort synthesis of bacteriophage lytic proteins against streptococcus pneumoniae in the chloroplast of chlamydomonas reinhardtii
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5552482/
https://www.ncbi.nlm.nih.gov/pubmed/28160380
http://dx.doi.org/10.1111/pbi.12703
work_keys_str_mv AT stoffelslaura synthesisofbacteriophagelyticproteinsagainststreptococcuspneumoniaeinthechloroplastofchlamydomonasreinhardtii
AT taunthenryn synthesisofbacteriophagelyticproteinsagainststreptococcuspneumoniaeinthechloroplastofchlamydomonasreinhardtii
AT charalambousbambos synthesisofbacteriophagelyticproteinsagainststreptococcuspneumoniaeinthechloroplastofchlamydomonasreinhardtii
AT purtonsaul synthesisofbacteriophagelyticproteinsagainststreptococcuspneumoniaeinthechloroplastofchlamydomonasreinhardtii