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Two Negative-Strand RNA Viruses Identified in Watermelon Represent a Novel Clade in the Order Bunyavirales

Two novel negative-sense, single-stranded (ss) RNA viruses were identified in watermelon plants and named watermelon crinkle leaf-associated virus 1 and 2 (WCLaV-1 and -2), respectively. The multipartite genomes consist of three RNA molecules of ~6.8, 1.4, and 1.3 kb. The genomes and the deduced pro...

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Autores principales: Xin, Min, Cao, Mengji, Liu, Wenwen, Ren, Yingdang, Zhou, Xueping, Wang, Xifeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5552725/
https://www.ncbi.nlm.nih.gov/pubmed/28848524
http://dx.doi.org/10.3389/fmicb.2017.01514
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author Xin, Min
Cao, Mengji
Liu, Wenwen
Ren, Yingdang
Zhou, Xueping
Wang, Xifeng
author_facet Xin, Min
Cao, Mengji
Liu, Wenwen
Ren, Yingdang
Zhou, Xueping
Wang, Xifeng
author_sort Xin, Min
collection PubMed
description Two novel negative-sense, single-stranded (ss) RNA viruses were identified in watermelon plants and named watermelon crinkle leaf-associated virus 1 and 2 (WCLaV-1 and -2), respectively. The multipartite genomes consist of three RNA molecules of ~6.8, 1.4, and 1.3 kb. The genomes and the deduced proteins of RNA1 and RNA3 show features resembling those of members in the genus Phlebovirus and Tenuivirus; however, the predicted proteins encoded by RNA2 are related to the movement protein (MP) in the genus Ophiovirus and Emaravirus. Furthermore, these two viruses define a novel clade in the family Phenuiviridae, order Bunyavirales, which is phylogenetically related to the viruses in the above four genera. Moreover, after mechanical inoculation with WCLaV-1 seedlings of the natural host watermelon plants develop crinkling similar to those observed in the field. These findings enhance our understanding of the evolution and the classification of ssRNA viruses.
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spelling pubmed-55527252017-08-28 Two Negative-Strand RNA Viruses Identified in Watermelon Represent a Novel Clade in the Order Bunyavirales Xin, Min Cao, Mengji Liu, Wenwen Ren, Yingdang Zhou, Xueping Wang, Xifeng Front Microbiol Microbiology Two novel negative-sense, single-stranded (ss) RNA viruses were identified in watermelon plants and named watermelon crinkle leaf-associated virus 1 and 2 (WCLaV-1 and -2), respectively. The multipartite genomes consist of three RNA molecules of ~6.8, 1.4, and 1.3 kb. The genomes and the deduced proteins of RNA1 and RNA3 show features resembling those of members in the genus Phlebovirus and Tenuivirus; however, the predicted proteins encoded by RNA2 are related to the movement protein (MP) in the genus Ophiovirus and Emaravirus. Furthermore, these two viruses define a novel clade in the family Phenuiviridae, order Bunyavirales, which is phylogenetically related to the viruses in the above four genera. Moreover, after mechanical inoculation with WCLaV-1 seedlings of the natural host watermelon plants develop crinkling similar to those observed in the field. These findings enhance our understanding of the evolution and the classification of ssRNA viruses. Frontiers Media S.A. 2017-08-09 /pmc/articles/PMC5552725/ /pubmed/28848524 http://dx.doi.org/10.3389/fmicb.2017.01514 Text en Copyright © 2017 Xin, Cao, Liu, Ren, Zhou and Wang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Xin, Min
Cao, Mengji
Liu, Wenwen
Ren, Yingdang
Zhou, Xueping
Wang, Xifeng
Two Negative-Strand RNA Viruses Identified in Watermelon Represent a Novel Clade in the Order Bunyavirales
title Two Negative-Strand RNA Viruses Identified in Watermelon Represent a Novel Clade in the Order Bunyavirales
title_full Two Negative-Strand RNA Viruses Identified in Watermelon Represent a Novel Clade in the Order Bunyavirales
title_fullStr Two Negative-Strand RNA Viruses Identified in Watermelon Represent a Novel Clade in the Order Bunyavirales
title_full_unstemmed Two Negative-Strand RNA Viruses Identified in Watermelon Represent a Novel Clade in the Order Bunyavirales
title_short Two Negative-Strand RNA Viruses Identified in Watermelon Represent a Novel Clade in the Order Bunyavirales
title_sort two negative-strand rna viruses identified in watermelon represent a novel clade in the order bunyavirales
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5552725/
https://www.ncbi.nlm.nih.gov/pubmed/28848524
http://dx.doi.org/10.3389/fmicb.2017.01514
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