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Transcriptional regulation of cpsQ‐mfpABC and mfpABC by CalR in Vibrio parahaemolyticus

The cpsQ‐mfpABC locus is transcribed as two operons, i.e., cpsQ‐mfpABC and mfpABC, in Vibrio parahaemolyticus, and both of them are all required for biofilm formation. CalR belongs to the LysR‐type transcriptional regulator family, and was originally identified as a repressor of the swarming motilit...

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Autores principales: Gao, He, Zhang, Lingyu, Osei‐Adjei, George, Yang, Wenhui, Zhou, Dongsheng, Huang, Xinxiang, Yang, Huiying, Yin, Zhe, Zhang, Yiquan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5552913/
https://www.ncbi.nlm.nih.gov/pubmed/28318117
http://dx.doi.org/10.1002/mbo3.470
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author Gao, He
Zhang, Lingyu
Osei‐Adjei, George
Yang, Wenhui
Zhou, Dongsheng
Huang, Xinxiang
Yang, Huiying
Yin, Zhe
Zhang, Yiquan
author_facet Gao, He
Zhang, Lingyu
Osei‐Adjei, George
Yang, Wenhui
Zhou, Dongsheng
Huang, Xinxiang
Yang, Huiying
Yin, Zhe
Zhang, Yiquan
author_sort Gao, He
collection PubMed
description The cpsQ‐mfpABC locus is transcribed as two operons, i.e., cpsQ‐mfpABC and mfpABC, in Vibrio parahaemolyticus, and both of them are all required for biofilm formation. CalR belongs to the LysR‐type transcriptional regulator family, and was originally identified as a repressor of the swarming motility and T3SS1 genes expression in V. parahaemolyticus. In the present work, a combination of qRT‐PCR, primer extension, LacZ fusion expression, electrophoretic mobility shift assay, and DNase I footprinting assays were employed to elucidate the regulatory mechanisms of cpsQ‐mfpABC and mfpABC by CalR. One transcription start site for each operon was detected and their activities were activated by CalR. His‐CalR protected two DNA regions upstream of mfpABC against DNase I digestion, but no binding sites were detected in the promoter region of cpsQ‐mfpABC, suggesting a direct and an indirect regulatory manner for mfpABC and cpsQ‐mfpABC transcription by CalR, respectively. Collectively, the results presented here confirmed a new physiological role for CalR that acts as an activator for cpsQ‐mfpABC and mfpABC transcription.
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spelling pubmed-55529132017-08-15 Transcriptional regulation of cpsQ‐mfpABC and mfpABC by CalR in Vibrio parahaemolyticus Gao, He Zhang, Lingyu Osei‐Adjei, George Yang, Wenhui Zhou, Dongsheng Huang, Xinxiang Yang, Huiying Yin, Zhe Zhang, Yiquan Microbiologyopen Original Research The cpsQ‐mfpABC locus is transcribed as two operons, i.e., cpsQ‐mfpABC and mfpABC, in Vibrio parahaemolyticus, and both of them are all required for biofilm formation. CalR belongs to the LysR‐type transcriptional regulator family, and was originally identified as a repressor of the swarming motility and T3SS1 genes expression in V. parahaemolyticus. In the present work, a combination of qRT‐PCR, primer extension, LacZ fusion expression, electrophoretic mobility shift assay, and DNase I footprinting assays were employed to elucidate the regulatory mechanisms of cpsQ‐mfpABC and mfpABC by CalR. One transcription start site for each operon was detected and their activities were activated by CalR. His‐CalR protected two DNA regions upstream of mfpABC against DNase I digestion, but no binding sites were detected in the promoter region of cpsQ‐mfpABC, suggesting a direct and an indirect regulatory manner for mfpABC and cpsQ‐mfpABC transcription by CalR, respectively. Collectively, the results presented here confirmed a new physiological role for CalR that acts as an activator for cpsQ‐mfpABC and mfpABC transcription. John Wiley and Sons Inc. 2017-03-20 /pmc/articles/PMC5552913/ /pubmed/28318117 http://dx.doi.org/10.1002/mbo3.470 Text en © 2017 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
Gao, He
Zhang, Lingyu
Osei‐Adjei, George
Yang, Wenhui
Zhou, Dongsheng
Huang, Xinxiang
Yang, Huiying
Yin, Zhe
Zhang, Yiquan
Transcriptional regulation of cpsQ‐mfpABC and mfpABC by CalR in Vibrio parahaemolyticus
title Transcriptional regulation of cpsQ‐mfpABC and mfpABC by CalR in Vibrio parahaemolyticus
title_full Transcriptional regulation of cpsQ‐mfpABC and mfpABC by CalR in Vibrio parahaemolyticus
title_fullStr Transcriptional regulation of cpsQ‐mfpABC and mfpABC by CalR in Vibrio parahaemolyticus
title_full_unstemmed Transcriptional regulation of cpsQ‐mfpABC and mfpABC by CalR in Vibrio parahaemolyticus
title_short Transcriptional regulation of cpsQ‐mfpABC and mfpABC by CalR in Vibrio parahaemolyticus
title_sort transcriptional regulation of cpsq‐mfpabc and mfpabc by calr in vibrio parahaemolyticus
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5552913/
https://www.ncbi.nlm.nih.gov/pubmed/28318117
http://dx.doi.org/10.1002/mbo3.470
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