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Effect and mechanism of calpains on pediatric lobar pneumonia
Lobar pneumonia, one of the community-acquired pneumonia (CAP), is a common pediatric low respiratory tract infection. Calpains are Ca(2+)-activated cysteine proteases whose activation mechanism is elusive. The present study was undertaken to detect the role and mechanism of calpains in pediatric lo...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5553339/ https://www.ncbi.nlm.nih.gov/pubmed/27786573 http://dx.doi.org/10.1080/21655979.2016.1234544 |
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author | Yin, Genquan Zeng, Qiang Zhao, Haijin Wu, Peiqiong Cai, Shaoxi Deng, Li Jiang, Wenhui |
author_facet | Yin, Genquan Zeng, Qiang Zhao, Haijin Wu, Peiqiong Cai, Shaoxi Deng, Li Jiang, Wenhui |
author_sort | Yin, Genquan |
collection | PubMed |
description | Lobar pneumonia, one of the community-acquired pneumonia (CAP), is a common pediatric low respiratory tract infection. Calpains are Ca(2+)-activated cysteine proteases whose activation mechanism is elusive. The present study was undertaken to detect the role and mechanism of calpains in pediatric lobar pneumonia. The human acute lung infection model (ALIM) was constructed and infected by Streptococcus. Enzyme-linked immunosorbent assay (ELISA) was used to measure interleukin (IL)-6, IL-8 and tumor necrosis factor (TNF)-α. We observed the lactate dehydrogenase (LDH) release, calpains activity and calpain inhibitor effects in ALIM. The expression of proliferating cell nuclear antigen (PCNA) protein was quantified by western blotting. Then the effects of calpain 1 and 2 knockdown on expressions of inflammation factors and PCNA protein, LDH release and apoptosis were evaluated in lung MRC-5 cells. In constructed ALIM, expressions of IL-6 (P < 0.01), IL-8 (P < 0.01), TNF-α (P < 0.05) and PCNA protein (P < 0.05) were significantly reduced by the calpain inhibitor. Expressions of IL-6, IL-8, TNF-α, PCNA protein and relative LDH release were statistically reduced by the small interfering (si) RNA-calpain 1 and 2 in MRC-5 cells (P < 0.05). Calpains silence increased apoptotic cells from 5% (negative control) to more than 20% in MRC-5 cells. The present study suggests that calpains possess a significant effect on inflammations, cell proliferation and apoptosis. Suppression of calpains may provide a potential therapeutic target of lobar pneumonia. |
format | Online Article Text |
id | pubmed-5553339 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-55533392017-10-27 Effect and mechanism of calpains on pediatric lobar pneumonia Yin, Genquan Zeng, Qiang Zhao, Haijin Wu, Peiqiong Cai, Shaoxi Deng, Li Jiang, Wenhui Bioengineered Research Paper Lobar pneumonia, one of the community-acquired pneumonia (CAP), is a common pediatric low respiratory tract infection. Calpains are Ca(2+)-activated cysteine proteases whose activation mechanism is elusive. The present study was undertaken to detect the role and mechanism of calpains in pediatric lobar pneumonia. The human acute lung infection model (ALIM) was constructed and infected by Streptococcus. Enzyme-linked immunosorbent assay (ELISA) was used to measure interleukin (IL)-6, IL-8 and tumor necrosis factor (TNF)-α. We observed the lactate dehydrogenase (LDH) release, calpains activity and calpain inhibitor effects in ALIM. The expression of proliferating cell nuclear antigen (PCNA) protein was quantified by western blotting. Then the effects of calpain 1 and 2 knockdown on expressions of inflammation factors and PCNA protein, LDH release and apoptosis were evaluated in lung MRC-5 cells. In constructed ALIM, expressions of IL-6 (P < 0.01), IL-8 (P < 0.01), TNF-α (P < 0.05) and PCNA protein (P < 0.05) were significantly reduced by the calpain inhibitor. Expressions of IL-6, IL-8, TNF-α, PCNA protein and relative LDH release were statistically reduced by the small interfering (si) RNA-calpain 1 and 2 in MRC-5 cells (P < 0.05). Calpains silence increased apoptotic cells from 5% (negative control) to more than 20% in MRC-5 cells. The present study suggests that calpains possess a significant effect on inflammations, cell proliferation and apoptosis. Suppression of calpains may provide a potential therapeutic target of lobar pneumonia. Taylor & Francis 2016-10-27 /pmc/articles/PMC5553339/ /pubmed/27786573 http://dx.doi.org/10.1080/21655979.2016.1234544 Text en © Genquan Yin, Qiang Zeng, Haijin Zhao, Peiqiong Wu, Shaoxi Cai, Li Deng, and Wenhui. Published with license by Taylor & Francis http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted. |
spellingShingle | Research Paper Yin, Genquan Zeng, Qiang Zhao, Haijin Wu, Peiqiong Cai, Shaoxi Deng, Li Jiang, Wenhui Effect and mechanism of calpains on pediatric lobar pneumonia |
title | Effect and mechanism of calpains on pediatric lobar pneumonia |
title_full | Effect and mechanism of calpains on pediatric lobar pneumonia |
title_fullStr | Effect and mechanism of calpains on pediatric lobar pneumonia |
title_full_unstemmed | Effect and mechanism of calpains on pediatric lobar pneumonia |
title_short | Effect and mechanism of calpains on pediatric lobar pneumonia |
title_sort | effect and mechanism of calpains on pediatric lobar pneumonia |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5553339/ https://www.ncbi.nlm.nih.gov/pubmed/27786573 http://dx.doi.org/10.1080/21655979.2016.1234544 |
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