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Combining NMR and small angle X-ray scattering for the study of biomolecular structure and dynamics
Small-angle X-ray scattering (SAXS) and Nuclear Magnetic Resonance (NMR) are established methods to analyze the structure and structural transitions of biological macromolecules in solution. Both methods are directly applicable to near-native macromolecular solutions and allow one to study structura...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5553349/ https://www.ncbi.nlm.nih.gov/pubmed/28501583 http://dx.doi.org/10.1016/j.abb.2017.05.005 |
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author | Mertens, Haydyn D.T. Svergun, Dmitri I. |
author_facet | Mertens, Haydyn D.T. Svergun, Dmitri I. |
author_sort | Mertens, Haydyn D.T. |
collection | PubMed |
description | Small-angle X-ray scattering (SAXS) and Nuclear Magnetic Resonance (NMR) are established methods to analyze the structure and structural transitions of biological macromolecules in solution. Both methods are directly applicable to near-native macromolecular solutions and allow one to study structural responses to physical and chemical changes or ligand additions. Whereas SAXS is applied to elucidate overall structure, interactions and flexibility over a wide range of particle sizes, NMR yields atomic resolution detail for moderately sized macromolecules. NMR is arguably the most powerful technique for the experimental analysis of dynamics. The joint application of these two highly complementary techniques provides an extremely useful approach that facilitates comprehensive characterization of biomacromolecular solutions. |
format | Online Article Text |
id | pubmed-5553349 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-55533492017-08-22 Combining NMR and small angle X-ray scattering for the study of biomolecular structure and dynamics Mertens, Haydyn D.T. Svergun, Dmitri I. Arch Biochem Biophys Article Small-angle X-ray scattering (SAXS) and Nuclear Magnetic Resonance (NMR) are established methods to analyze the structure and structural transitions of biological macromolecules in solution. Both methods are directly applicable to near-native macromolecular solutions and allow one to study structural responses to physical and chemical changes or ligand additions. Whereas SAXS is applied to elucidate overall structure, interactions and flexibility over a wide range of particle sizes, NMR yields atomic resolution detail for moderately sized macromolecules. NMR is arguably the most powerful technique for the experimental analysis of dynamics. The joint application of these two highly complementary techniques provides an extremely useful approach that facilitates comprehensive characterization of biomacromolecular solutions. Elsevier 2017-08-15 /pmc/articles/PMC5553349/ /pubmed/28501583 http://dx.doi.org/10.1016/j.abb.2017.05.005 Text en © 2017 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Mertens, Haydyn D.T. Svergun, Dmitri I. Combining NMR and small angle X-ray scattering for the study of biomolecular structure and dynamics |
title | Combining NMR and small angle X-ray scattering for the study of biomolecular structure and dynamics |
title_full | Combining NMR and small angle X-ray scattering for the study of biomolecular structure and dynamics |
title_fullStr | Combining NMR and small angle X-ray scattering for the study of biomolecular structure and dynamics |
title_full_unstemmed | Combining NMR and small angle X-ray scattering for the study of biomolecular structure and dynamics |
title_short | Combining NMR and small angle X-ray scattering for the study of biomolecular structure and dynamics |
title_sort | combining nmr and small angle x-ray scattering for the study of biomolecular structure and dynamics |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5553349/ https://www.ncbi.nlm.nih.gov/pubmed/28501583 http://dx.doi.org/10.1016/j.abb.2017.05.005 |
work_keys_str_mv | AT mertenshaydyndt combiningnmrandsmallanglexrayscatteringforthestudyofbiomolecularstructureanddynamics AT svergundmitrii combiningnmrandsmallanglexrayscatteringforthestudyofbiomolecularstructureanddynamics |