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Efficient Delivery of Quantum Dots into the Cytosol of Cells Using Cell-Penetrating Poly(disulfide)s
[Image: see text] Quantum dots (QDs) are extremely bright, photostable, nanometer particles broadly used to investigate single molecule dynamics in vitro. However, the use of QDs in vivo to investigate single molecule dynamics is impaired by the absence of an efficient way to chemically deliver them...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical
Society
2017
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5553715/ https://www.ncbi.nlm.nih.gov/pubmed/28741941 http://dx.doi.org/10.1021/jacs.7b02952 |
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author | Derivery, Emmanuel Bartolami, Eline Matile, Stefan Gonzalez-Gaitan, Marcos |
author_facet | Derivery, Emmanuel Bartolami, Eline Matile, Stefan Gonzalez-Gaitan, Marcos |
author_sort | Derivery, Emmanuel |
collection | PubMed |
description | [Image: see text] Quantum dots (QDs) are extremely bright, photostable, nanometer particles broadly used to investigate single molecule dynamics in vitro. However, the use of QDs in vivo to investigate single molecule dynamics is impaired by the absence of an efficient way to chemically deliver them into the cytosol of cells. Indeed, current methods (using cell-penetrating peptides for instance) provide very low yields: QDs stay at the plasma membrane or are trapped in endosomes. Here, we introduce a technology based on cell-penetrating poly(disulfide)s that solves this problem: we deliver about 70 QDs per cell, and 90% appear to freely diffuse in the cytosol. Furthermore, these QDs can be functionalized, carrying GFP or anti-GFP nanobodies for instance. Our technology thus paves the way toward single molecule imaging in cells and living animals, allowing to probe biophysical properties of the cytosol. |
format | Online Article Text |
id | pubmed-5553715 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | American Chemical
Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-55537152017-08-14 Efficient Delivery of Quantum Dots into the Cytosol of Cells Using Cell-Penetrating Poly(disulfide)s Derivery, Emmanuel Bartolami, Eline Matile, Stefan Gonzalez-Gaitan, Marcos J Am Chem Soc [Image: see text] Quantum dots (QDs) are extremely bright, photostable, nanometer particles broadly used to investigate single molecule dynamics in vitro. However, the use of QDs in vivo to investigate single molecule dynamics is impaired by the absence of an efficient way to chemically deliver them into the cytosol of cells. Indeed, current methods (using cell-penetrating peptides for instance) provide very low yields: QDs stay at the plasma membrane or are trapped in endosomes. Here, we introduce a technology based on cell-penetrating poly(disulfide)s that solves this problem: we deliver about 70 QDs per cell, and 90% appear to freely diffuse in the cytosol. Furthermore, these QDs can be functionalized, carrying GFP or anti-GFP nanobodies for instance. Our technology thus paves the way toward single molecule imaging in cells and living animals, allowing to probe biophysical properties of the cytosol. American Chemical Society 2017-07-25 2017-08-02 /pmc/articles/PMC5553715/ /pubmed/28741941 http://dx.doi.org/10.1021/jacs.7b02952 Text en Copyright © 2017 American Chemical Society This is an open access article published under a Creative Commons Attribution (CC-BY) License (http://pubs.acs.org/page/policy/authorchoice_ccby_termsofuse.html) , which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited. |
spellingShingle | Derivery, Emmanuel Bartolami, Eline Matile, Stefan Gonzalez-Gaitan, Marcos Efficient Delivery of Quantum Dots into the Cytosol of Cells Using Cell-Penetrating Poly(disulfide)s |
title | Efficient
Delivery of Quantum Dots into the Cytosol
of Cells Using Cell-Penetrating Poly(disulfide)s |
title_full | Efficient
Delivery of Quantum Dots into the Cytosol
of Cells Using Cell-Penetrating Poly(disulfide)s |
title_fullStr | Efficient
Delivery of Quantum Dots into the Cytosol
of Cells Using Cell-Penetrating Poly(disulfide)s |
title_full_unstemmed | Efficient
Delivery of Quantum Dots into the Cytosol
of Cells Using Cell-Penetrating Poly(disulfide)s |
title_short | Efficient
Delivery of Quantum Dots into the Cytosol
of Cells Using Cell-Penetrating Poly(disulfide)s |
title_sort | efficient
delivery of quantum dots into the cytosol
of cells using cell-penetrating poly(disulfide)s |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5553715/ https://www.ncbi.nlm.nih.gov/pubmed/28741941 http://dx.doi.org/10.1021/jacs.7b02952 |
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