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Rattus norvegicus BN/SHR liver and heart left ventricle ribosomal RNA depleted directional RNA sequencing

OBJECTIVE: The spontaneously hypertensive rat strain is a frequently used disease model. In a previous study, we measured translational efficiency from this strain and BN-Lx animals. Here, we describe long RNA sequencing reads from ribosomal RNA depleted samples from the same animals. This data can...

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Autores principales: Wyler, Emanuel, van Heesch, Sebastiaan, Adami, Eleonora, Hubner, Norbert, Landthaler, Markus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5553801/
https://www.ncbi.nlm.nih.gov/pubmed/28800773
http://dx.doi.org/10.1186/s13104-017-2716-4
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author Wyler, Emanuel
van Heesch, Sebastiaan
Adami, Eleonora
Hubner, Norbert
Landthaler, Markus
author_facet Wyler, Emanuel
van Heesch, Sebastiaan
Adami, Eleonora
Hubner, Norbert
Landthaler, Markus
author_sort Wyler, Emanuel
collection PubMed
description OBJECTIVE: The spontaneously hypertensive rat strain is a frequently used disease model. In a previous study, we measured translational efficiency from this strain and BN-Lx animals. Here, we describe long RNA sequencing reads from ribosomal RNA depleted samples from the same animals. This data can be used to investigate splicing-related events. RESULTS: RNA was extracted from rat liver and heart left ventricle from BN-Lx and SHR/Ola rats in biological replicates. Ribosomal RNA was removed and the samples subjected to directional high-throughput RNA-sequencing. Read and alignment statistics indicate high quality of the data. The raw sequencing reads are freely available on the NCBI short read archive and can be used for further research on tissue and strain differences, or analysed together with other published high-throughput data from the same animals.
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spelling pubmed-55538012017-08-15 Rattus norvegicus BN/SHR liver and heart left ventricle ribosomal RNA depleted directional RNA sequencing Wyler, Emanuel van Heesch, Sebastiaan Adami, Eleonora Hubner, Norbert Landthaler, Markus BMC Res Notes Research Note OBJECTIVE: The spontaneously hypertensive rat strain is a frequently used disease model. In a previous study, we measured translational efficiency from this strain and BN-Lx animals. Here, we describe long RNA sequencing reads from ribosomal RNA depleted samples from the same animals. This data can be used to investigate splicing-related events. RESULTS: RNA was extracted from rat liver and heart left ventricle from BN-Lx and SHR/Ola rats in biological replicates. Ribosomal RNA was removed and the samples subjected to directional high-throughput RNA-sequencing. Read and alignment statistics indicate high quality of the data. The raw sequencing reads are freely available on the NCBI short read archive and can be used for further research on tissue and strain differences, or analysed together with other published high-throughput data from the same animals. BioMed Central 2017-08-11 /pmc/articles/PMC5553801/ /pubmed/28800773 http://dx.doi.org/10.1186/s13104-017-2716-4 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Note
Wyler, Emanuel
van Heesch, Sebastiaan
Adami, Eleonora
Hubner, Norbert
Landthaler, Markus
Rattus norvegicus BN/SHR liver and heart left ventricle ribosomal RNA depleted directional RNA sequencing
title Rattus norvegicus BN/SHR liver and heart left ventricle ribosomal RNA depleted directional RNA sequencing
title_full Rattus norvegicus BN/SHR liver and heart left ventricle ribosomal RNA depleted directional RNA sequencing
title_fullStr Rattus norvegicus BN/SHR liver and heart left ventricle ribosomal RNA depleted directional RNA sequencing
title_full_unstemmed Rattus norvegicus BN/SHR liver and heart left ventricle ribosomal RNA depleted directional RNA sequencing
title_short Rattus norvegicus BN/SHR liver and heart left ventricle ribosomal RNA depleted directional RNA sequencing
title_sort rattus norvegicus bn/shr liver and heart left ventricle ribosomal rna depleted directional rna sequencing
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5553801/
https://www.ncbi.nlm.nih.gov/pubmed/28800773
http://dx.doi.org/10.1186/s13104-017-2716-4
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