Using local chromatin structure to improve CRISPR/Cas9 efficiency in zebrafish

Although the CRISPR/Cas9 has been successfully applied in zebrafish, considerable variations in efficiency have been observed for different gRNAs. The workload and cost of zebrafish mutant screening is largely dependent on the mutation rate of injected embryos; therefore, selecting more effective gR...

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Detalles Bibliográficos
Autores principales: Chen, Yunru, Zeng, Shiyang, Hu, Ruikun, Wang, Xiangxiu, Huang, Weilai, Liu, Jiangfang, Wang, Luying, Liu, Guifen, Cao, Ying, Zhang, Yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5553855/
https://www.ncbi.nlm.nih.gov/pubmed/28800611
http://dx.doi.org/10.1371/journal.pone.0182528

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