Cargando…

Enveloped and non-enveloped viral-like particles in Trypanosoma cruzi epimastigotes

Electron microscopy is routinely used to identify viral infections in protozoan parasites. These viruses have been described as non-enveloped and icosahedral structures with a diameter of 30-60 nm. Most of them are classified within the non-segmented dsRNA Totiviridae family. We observed virus-like...

Descripción completa

Detalles Bibliográficos
Autores principales: Fernández-Presas, Ana María, Padilla-Noriega, Luis, Ingeborg-Becker, Robert, Lilia, Jiménez, José Agustín, Solano, Sandra, Delgado, Jose, Tato, Patricia, Molinari, José Luis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Instituto de Medicina Tropical 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5553942/
https://www.ncbi.nlm.nih.gov/pubmed/28793017
http://dx.doi.org/10.1590/S1678-9946201759046
_version_ 1783256702730108928
author Fernández-Presas, Ana María
Padilla-Noriega, Luis
Ingeborg-Becker,
Robert, Lilia
Jiménez, José Agustín
Solano, Sandra
Delgado, Jose
Tato, Patricia
Molinari, José Luis
author_facet Fernández-Presas, Ana María
Padilla-Noriega, Luis
Ingeborg-Becker,
Robert, Lilia
Jiménez, José Agustín
Solano, Sandra
Delgado, Jose
Tato, Patricia
Molinari, José Luis
author_sort Fernández-Presas, Ana María
collection PubMed
description Electron microscopy is routinely used to identify viral infections in protozoan parasites. These viruses have been described as non-enveloped and icosahedral structures with a diameter of 30-60 nm. Most of them are classified within the non-segmented dsRNA Totiviridae family. We observed virus-like particles (VLPs) through transmission electron microscopy in the cytoplasm of Trypanosoma cruzi epimastigotes grown in cultures. Clusters of electrodense enveloped VLPs having a diameter of 48 nm were also observed. These clusters appear to have been released from distended Golgi cisternae. Furthermore, a paracrystalline array of electrodense, non-enveloped VLPs (with a diameter of 32 nm) were found in distended Golgi cisternae or as smaller clusters at a distance from the RE or Golgi. We cannot rule out that the 48 nm enveloped VLPs belong to the ssRNA Flaviviridae family because they are within its size range. The localization of enveloped VLPs is consistent with the replication strategy of these viruses that transit through the Golgi to be released at the cell surface. Due to the size and shape of the 32 nm non-enveloped VLPs, we propose that they belong to the dsRNA Totiviridae family. This is the first description of cytoplasmic enveloped and non-enveloped VLPs in T. cruzi epimastigotes.
format Online
Article
Text
id pubmed-5553942
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Instituto de Medicina Tropical
record_format MEDLINE/PubMed
spelling pubmed-55539422017-08-17 Enveloped and non-enveloped viral-like particles in Trypanosoma cruzi epimastigotes Fernández-Presas, Ana María Padilla-Noriega, Luis Ingeborg-Becker, Robert, Lilia Jiménez, José Agustín Solano, Sandra Delgado, Jose Tato, Patricia Molinari, José Luis Rev Inst Med Trop Sao Paulo Original Article Electron microscopy is routinely used to identify viral infections in protozoan parasites. These viruses have been described as non-enveloped and icosahedral structures with a diameter of 30-60 nm. Most of them are classified within the non-segmented dsRNA Totiviridae family. We observed virus-like particles (VLPs) through transmission electron microscopy in the cytoplasm of Trypanosoma cruzi epimastigotes grown in cultures. Clusters of electrodense enveloped VLPs having a diameter of 48 nm were also observed. These clusters appear to have been released from distended Golgi cisternae. Furthermore, a paracrystalline array of electrodense, non-enveloped VLPs (with a diameter of 32 nm) were found in distended Golgi cisternae or as smaller clusters at a distance from the RE or Golgi. We cannot rule out that the 48 nm enveloped VLPs belong to the ssRNA Flaviviridae family because they are within its size range. The localization of enveloped VLPs is consistent with the replication strategy of these viruses that transit through the Golgi to be released at the cell surface. Due to the size and shape of the 32 nm non-enveloped VLPs, we propose that they belong to the dsRNA Totiviridae family. This is the first description of cytoplasmic enveloped and non-enveloped VLPs in T. cruzi epimastigotes. Instituto de Medicina Tropical 2017-08-07 /pmc/articles/PMC5553942/ /pubmed/28793017 http://dx.doi.org/10.1590/S1678-9946201759046 Text en http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Fernández-Presas, Ana María
Padilla-Noriega, Luis
Ingeborg-Becker,
Robert, Lilia
Jiménez, José Agustín
Solano, Sandra
Delgado, Jose
Tato, Patricia
Molinari, José Luis
Enveloped and non-enveloped viral-like particles in Trypanosoma cruzi epimastigotes
title Enveloped and non-enveloped viral-like particles in Trypanosoma cruzi epimastigotes
title_full Enveloped and non-enveloped viral-like particles in Trypanosoma cruzi epimastigotes
title_fullStr Enveloped and non-enveloped viral-like particles in Trypanosoma cruzi epimastigotes
title_full_unstemmed Enveloped and non-enveloped viral-like particles in Trypanosoma cruzi epimastigotes
title_short Enveloped and non-enveloped viral-like particles in Trypanosoma cruzi epimastigotes
title_sort enveloped and non-enveloped viral-like particles in trypanosoma cruzi epimastigotes
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5553942/
https://www.ncbi.nlm.nih.gov/pubmed/28793017
http://dx.doi.org/10.1590/S1678-9946201759046
work_keys_str_mv AT fernandezpresasanamaria envelopedandnonenvelopedvirallikeparticlesintrypanosomacruziepimastigotes
AT padillanoriegaluis envelopedandnonenvelopedvirallikeparticlesintrypanosomacruziepimastigotes
AT ingeborgbecker envelopedandnonenvelopedvirallikeparticlesintrypanosomacruziepimastigotes
AT robertlilia envelopedandnonenvelopedvirallikeparticlesintrypanosomacruziepimastigotes
AT jimenezjoseagustin envelopedandnonenvelopedvirallikeparticlesintrypanosomacruziepimastigotes
AT solanosandra envelopedandnonenvelopedvirallikeparticlesintrypanosomacruziepimastigotes
AT delgadojose envelopedandnonenvelopedvirallikeparticlesintrypanosomacruziepimastigotes
AT tatopatricia envelopedandnonenvelopedvirallikeparticlesintrypanosomacruziepimastigotes
AT molinarijoseluis envelopedandnonenvelopedvirallikeparticlesintrypanosomacruziepimastigotes