Cargando…
Dietary arginine affects the insulin signaling pathway, glucose metabolism and lipogenesis in juvenile blunt snout bream Megalobrama amblycephala
This study evaluated the mechanisms governing insulin resistance, glucose metabolism and lipogenesis in juvenile fish fed with graded levels of dietary arginine. The results showed that, compared with the control group (0.87%), 2.31% dietary arginine level resulted in the upregulation of the relativ...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5554147/ https://www.ncbi.nlm.nih.gov/pubmed/28801592 http://dx.doi.org/10.1038/s41598-017-06104-3 |
_version_ | 1783256734165368832 |
---|---|
author | Liang, Hualiang Habte-Tsion, Habte-Michael Ge, Xianping Ren, Mingchun Xie, Jun Miao, Linghong Zhou, Qunlan Lin, Yan Pan, Wenjing |
author_facet | Liang, Hualiang Habte-Tsion, Habte-Michael Ge, Xianping Ren, Mingchun Xie, Jun Miao, Linghong Zhou, Qunlan Lin, Yan Pan, Wenjing |
author_sort | Liang, Hualiang |
collection | PubMed |
description | This study evaluated the mechanisms governing insulin resistance, glucose metabolism and lipogenesis in juvenile fish fed with graded levels of dietary arginine. The results showed that, compared with the control group (0.87%), 2.31% dietary arginine level resulted in the upregulation of the relative gene expression of IRS-1, PI3K and Akt in the insulin signaling pathway, while 2.70% dietary arginine level led to inhibition of these genes. 1.62% dietary arginine level upregulated glycolysis by increasing GK mRNA level; 2.70% dietary arginine level upregulated gluconeogenesis and resulted in high plasma glucose content by increasing PEPCK and G6P mRNA level. Furthermore, 2.70% dietary arginine level significantly lowered GLUT2 and increased PK mRNA levels. 1.62% dietary arginine level significantly upregulated ACC, FAS and G6PDH mRNA levels in the fat synthesis pathway and resulted in high plasma TG content. These results indicate that 1.62% dietary arginine level improves glycolysis and fatty acid synthesis in juvenile blunt snout bream. However, 2.70% dietary arginine level results in high plasma glucose, which could lead to negative feedback of insulin resistance, including inhibition of IRS-1 mRNA levels and activation of gluconeogenesis-related gene expression. This mechanism seems to be different from mammals at the molecular level. |
format | Online Article Text |
id | pubmed-5554147 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-55541472017-08-15 Dietary arginine affects the insulin signaling pathway, glucose metabolism and lipogenesis in juvenile blunt snout bream Megalobrama amblycephala Liang, Hualiang Habte-Tsion, Habte-Michael Ge, Xianping Ren, Mingchun Xie, Jun Miao, Linghong Zhou, Qunlan Lin, Yan Pan, Wenjing Sci Rep Article This study evaluated the mechanisms governing insulin resistance, glucose metabolism and lipogenesis in juvenile fish fed with graded levels of dietary arginine. The results showed that, compared with the control group (0.87%), 2.31% dietary arginine level resulted in the upregulation of the relative gene expression of IRS-1, PI3K and Akt in the insulin signaling pathway, while 2.70% dietary arginine level led to inhibition of these genes. 1.62% dietary arginine level upregulated glycolysis by increasing GK mRNA level; 2.70% dietary arginine level upregulated gluconeogenesis and resulted in high plasma glucose content by increasing PEPCK and G6P mRNA level. Furthermore, 2.70% dietary arginine level significantly lowered GLUT2 and increased PK mRNA levels. 1.62% dietary arginine level significantly upregulated ACC, FAS and G6PDH mRNA levels in the fat synthesis pathway and resulted in high plasma TG content. These results indicate that 1.62% dietary arginine level improves glycolysis and fatty acid synthesis in juvenile blunt snout bream. However, 2.70% dietary arginine level results in high plasma glucose, which could lead to negative feedback of insulin resistance, including inhibition of IRS-1 mRNA levels and activation of gluconeogenesis-related gene expression. This mechanism seems to be different from mammals at the molecular level. Nature Publishing Group UK 2017-08-11 /pmc/articles/PMC5554147/ /pubmed/28801592 http://dx.doi.org/10.1038/s41598-017-06104-3 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Liang, Hualiang Habte-Tsion, Habte-Michael Ge, Xianping Ren, Mingchun Xie, Jun Miao, Linghong Zhou, Qunlan Lin, Yan Pan, Wenjing Dietary arginine affects the insulin signaling pathway, glucose metabolism and lipogenesis in juvenile blunt snout bream Megalobrama amblycephala |
title | Dietary arginine affects the insulin signaling pathway, glucose metabolism and lipogenesis in juvenile blunt snout bream Megalobrama amblycephala |
title_full | Dietary arginine affects the insulin signaling pathway, glucose metabolism and lipogenesis in juvenile blunt snout bream Megalobrama amblycephala |
title_fullStr | Dietary arginine affects the insulin signaling pathway, glucose metabolism and lipogenesis in juvenile blunt snout bream Megalobrama amblycephala |
title_full_unstemmed | Dietary arginine affects the insulin signaling pathway, glucose metabolism and lipogenesis in juvenile blunt snout bream Megalobrama amblycephala |
title_short | Dietary arginine affects the insulin signaling pathway, glucose metabolism and lipogenesis in juvenile blunt snout bream Megalobrama amblycephala |
title_sort | dietary arginine affects the insulin signaling pathway, glucose metabolism and lipogenesis in juvenile blunt snout bream megalobrama amblycephala |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5554147/ https://www.ncbi.nlm.nih.gov/pubmed/28801592 http://dx.doi.org/10.1038/s41598-017-06104-3 |
work_keys_str_mv | AT lianghualiang dietaryarginineaffectstheinsulinsignalingpathwayglucosemetabolismandlipogenesisinjuvenilebluntsnoutbreammegalobramaamblycephala AT habtetsionhabtemichael dietaryarginineaffectstheinsulinsignalingpathwayglucosemetabolismandlipogenesisinjuvenilebluntsnoutbreammegalobramaamblycephala AT gexianping dietaryarginineaffectstheinsulinsignalingpathwayglucosemetabolismandlipogenesisinjuvenilebluntsnoutbreammegalobramaamblycephala AT renmingchun dietaryarginineaffectstheinsulinsignalingpathwayglucosemetabolismandlipogenesisinjuvenilebluntsnoutbreammegalobramaamblycephala AT xiejun dietaryarginineaffectstheinsulinsignalingpathwayglucosemetabolismandlipogenesisinjuvenilebluntsnoutbreammegalobramaamblycephala AT miaolinghong dietaryarginineaffectstheinsulinsignalingpathwayglucosemetabolismandlipogenesisinjuvenilebluntsnoutbreammegalobramaamblycephala AT zhouqunlan dietaryarginineaffectstheinsulinsignalingpathwayglucosemetabolismandlipogenesisinjuvenilebluntsnoutbreammegalobramaamblycephala AT linyan dietaryarginineaffectstheinsulinsignalingpathwayglucosemetabolismandlipogenesisinjuvenilebluntsnoutbreammegalobramaamblycephala AT panwenjing dietaryarginineaffectstheinsulinsignalingpathwayglucosemetabolismandlipogenesisinjuvenilebluntsnoutbreammegalobramaamblycephala |