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Investigation into the stability and culturability of Chinese enterotypes

Although many gut microbial enterotypes have been reported in Europe, Africa and the U.S., their effects on human health are still not yet clear. Culturing gut microbial enterotypes in vitro will be helpful to study their effects and applications. Here, fecal samples from 13 healthy Chinese voluntee...

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Autores principales: Yin, Yeshi, Fan, Bin, Liu, Wei, Ren, Rongrong, Chen, Huahai, Bai, Shaofeng, Zhu, Liying, Sun, Gang, Yang, Yunsheng, Wang, Xin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5554170/
https://www.ncbi.nlm.nih.gov/pubmed/28801639
http://dx.doi.org/10.1038/s41598-017-08478-w
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author Yin, Yeshi
Fan, Bin
Liu, Wei
Ren, Rongrong
Chen, Huahai
Bai, Shaofeng
Zhu, Liying
Sun, Gang
Yang, Yunsheng
Wang, Xin
author_facet Yin, Yeshi
Fan, Bin
Liu, Wei
Ren, Rongrong
Chen, Huahai
Bai, Shaofeng
Zhu, Liying
Sun, Gang
Yang, Yunsheng
Wang, Xin
author_sort Yin, Yeshi
collection PubMed
description Although many gut microbial enterotypes have been reported in Europe, Africa and the U.S., their effects on human health are still not yet clear. Culturing gut microbial enterotypes in vitro will be helpful to study their effects and applications. Here, fecal samples from 13 healthy Chinese volunteers were collected and subjected to next-generation sequencing. The results showed that seven of these samples belong to the Bacteroides enterotype and another six to the Prevotella enterotype. Stability of these Chinese gut microbial enterotypes was also evaluated. Results showed that most of the tested volunteer gut microbiota to be very stable. For one volunteer, the bacterial community returned to the state it was in before intestinal lavage and antibiotics treatment after four months. XP medium was found effective for simulating the Bacteroides enterotype independent of the original gut microbial community in an in vitro chemostat culture system. Although, the Prevotella enterotype was not very well simulated in vitro, different culture elements selectively enriched different gut bacteria. Pectin and xylan were found to be related to the enrichment of the genera Bacteroides, Sutterella, and Flavonifractor in this chemostat culture system.
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spelling pubmed-55541702017-08-15 Investigation into the stability and culturability of Chinese enterotypes Yin, Yeshi Fan, Bin Liu, Wei Ren, Rongrong Chen, Huahai Bai, Shaofeng Zhu, Liying Sun, Gang Yang, Yunsheng Wang, Xin Sci Rep Article Although many gut microbial enterotypes have been reported in Europe, Africa and the U.S., their effects on human health are still not yet clear. Culturing gut microbial enterotypes in vitro will be helpful to study their effects and applications. Here, fecal samples from 13 healthy Chinese volunteers were collected and subjected to next-generation sequencing. The results showed that seven of these samples belong to the Bacteroides enterotype and another six to the Prevotella enterotype. Stability of these Chinese gut microbial enterotypes was also evaluated. Results showed that most of the tested volunteer gut microbiota to be very stable. For one volunteer, the bacterial community returned to the state it was in before intestinal lavage and antibiotics treatment after four months. XP medium was found effective for simulating the Bacteroides enterotype independent of the original gut microbial community in an in vitro chemostat culture system. Although, the Prevotella enterotype was not very well simulated in vitro, different culture elements selectively enriched different gut bacteria. Pectin and xylan were found to be related to the enrichment of the genera Bacteroides, Sutterella, and Flavonifractor in this chemostat culture system. Nature Publishing Group UK 2017-08-11 /pmc/articles/PMC5554170/ /pubmed/28801639 http://dx.doi.org/10.1038/s41598-017-08478-w Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Yin, Yeshi
Fan, Bin
Liu, Wei
Ren, Rongrong
Chen, Huahai
Bai, Shaofeng
Zhu, Liying
Sun, Gang
Yang, Yunsheng
Wang, Xin
Investigation into the stability and culturability of Chinese enterotypes
title Investigation into the stability and culturability of Chinese enterotypes
title_full Investigation into the stability and culturability of Chinese enterotypes
title_fullStr Investigation into the stability and culturability of Chinese enterotypes
title_full_unstemmed Investigation into the stability and culturability of Chinese enterotypes
title_short Investigation into the stability and culturability of Chinese enterotypes
title_sort investigation into the stability and culturability of chinese enterotypes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5554170/
https://www.ncbi.nlm.nih.gov/pubmed/28801639
http://dx.doi.org/10.1038/s41598-017-08478-w
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