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Efficient generation of conditional knockout mice via sequential introduction of lox sites

Conditional knockout using Cre/lox is essential for functional analysis of genes. CRISPR/Cas in combination with two sets of guide RNAs and a single-stranded oligonucleotide enables simultaneous insertion of two lox sequences. However, this method induces double-strand breaks at two sites on the sam...

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Detalles Bibliográficos
Autores principales:	Horii, Takuro, Morita, Sumiyo, Kimura, Mika, Terawaki, Naomi, Shibutani, Mihiro, Hatada, Izuho
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2017
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5554182/ https://www.ncbi.nlm.nih.gov/pubmed/28801621 http://dx.doi.org/10.1038/s41598-017-08496-8

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