Atomic Force Microscopy Reveals the Dynamic Morphology of Fenestrations in Live Liver Sinusoidal Endothelial Cells

Here, we report an atomic force microscopy (AFM)-based imaging method for resolving the fine nanostructures (e.g., fenestrations) in the membranes of live primary murine liver sinusoidal endothelial cells (LSECs). From data on topographical and nanomechanical properties of the selected cell areas co...

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Autores principales: Zapotoczny, B., Szafranska, K., Owczarczyk, K., Kus, E., Chlopicki, S., Szymonski, M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5554186/
https://www.ncbi.nlm.nih.gov/pubmed/28801568
http://dx.doi.org/10.1038/s41598-017-08555-0
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author Zapotoczny, B.
Szafranska, K.
Owczarczyk, K.
Kus, E.
Chlopicki, S.
Szymonski, M.
author_facet Zapotoczny, B.
Szafranska, K.
Owczarczyk, K.
Kus, E.
Chlopicki, S.
Szymonski, M.
author_sort Zapotoczny, B.
collection PubMed
description Here, we report an atomic force microscopy (AFM)-based imaging method for resolving the fine nanostructures (e.g., fenestrations) in the membranes of live primary murine liver sinusoidal endothelial cells (LSECs). From data on topographical and nanomechanical properties of the selected cell areas collected within 1 min, we traced the dynamic rearrangement of the cell actin cytoskeleton connected with the formation or closing of cell fenestrations, both in non-stimulated LSECs as well as in response to cytochalasin B and antimycin A. In conclusion, AFM-based imaging permitted the near real-time measurements of dynamic changes in fenestrations in live LSECs.
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spelling pubmed-55541862017-08-15 Atomic Force Microscopy Reveals the Dynamic Morphology of Fenestrations in Live Liver Sinusoidal Endothelial Cells Zapotoczny, B. Szafranska, K. Owczarczyk, K. Kus, E. Chlopicki, S. Szymonski, M. Sci Rep Article Here, we report an atomic force microscopy (AFM)-based imaging method for resolving the fine nanostructures (e.g., fenestrations) in the membranes of live primary murine liver sinusoidal endothelial cells (LSECs). From data on topographical and nanomechanical properties of the selected cell areas collected within 1 min, we traced the dynamic rearrangement of the cell actin cytoskeleton connected with the formation or closing of cell fenestrations, both in non-stimulated LSECs as well as in response to cytochalasin B and antimycin A. In conclusion, AFM-based imaging permitted the near real-time measurements of dynamic changes in fenestrations in live LSECs. Nature Publishing Group UK 2017-08-11 /pmc/articles/PMC5554186/ /pubmed/28801568 http://dx.doi.org/10.1038/s41598-017-08555-0 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Zapotoczny, B.
Szafranska, K.
Owczarczyk, K.
Kus, E.
Chlopicki, S.
Szymonski, M.
Atomic Force Microscopy Reveals the Dynamic Morphology of Fenestrations in Live Liver Sinusoidal Endothelial Cells
title Atomic Force Microscopy Reveals the Dynamic Morphology of Fenestrations in Live Liver Sinusoidal Endothelial Cells
title_full Atomic Force Microscopy Reveals the Dynamic Morphology of Fenestrations in Live Liver Sinusoidal Endothelial Cells
title_fullStr Atomic Force Microscopy Reveals the Dynamic Morphology of Fenestrations in Live Liver Sinusoidal Endothelial Cells
title_full_unstemmed Atomic Force Microscopy Reveals the Dynamic Morphology of Fenestrations in Live Liver Sinusoidal Endothelial Cells
title_short Atomic Force Microscopy Reveals the Dynamic Morphology of Fenestrations in Live Liver Sinusoidal Endothelial Cells
title_sort atomic force microscopy reveals the dynamic morphology of fenestrations in live liver sinusoidal endothelial cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5554186/
https://www.ncbi.nlm.nih.gov/pubmed/28801568
http://dx.doi.org/10.1038/s41598-017-08555-0
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