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Human Mesenchymal Stromal Cell Sheet Enhances Allograft Repair in a Mouse Model
To determine whether cell sheets generated with long-term passaged (P10) aging human mesenchymal stromal cells (MSCs) could be used for bone tissue regeneration as tissue engineered periosteum in a femoral allograft mouse model similar to fresh passaged (P3) young MSCs. At 3 weeks after transplantat...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5554246/ https://www.ncbi.nlm.nih.gov/pubmed/28801687 http://dx.doi.org/10.1038/s41598-017-08804-2 |
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author | Shang, Xifu Shu, Bing Wang, Yongjun Luo, Zhengliang Wang, Guangxi Barton, Shane Morandi, Massimo Max Kevil, Christopher Dong, Yufeng |
author_facet | Shang, Xifu Shu, Bing Wang, Yongjun Luo, Zhengliang Wang, Guangxi Barton, Shane Morandi, Massimo Max Kevil, Christopher Dong, Yufeng |
author_sort | Shang, Xifu |
collection | PubMed |
description | To determine whether cell sheets generated with long-term passaged (P10) aging human mesenchymal stromal cells (MSCs) could be used for bone tissue regeneration as tissue engineered periosteum in a femoral allograft mouse model similar to fresh passaged (P3) young MSCs. At 3 weeks after transplantation of MSC sheets, results showed more bony callus formed between allograft and host bone ends in both young P3 MSC and aged P10 MSC sheet-wrapped groups when compared to allograft alone. At 6 weeks, while both MSC sheet-wrapped allografts showed more bony callus formation when compared to allograft alone groups, the bony callus size in aged P10 MSC sheet groups was significantly less than young P3 MSC sheet groups. Biomechanical testing confirmed that P3 MSC sheet-grafted femurs had the highest biomechanical strength in the three groups. Histology sections showed that the area of the chondriod callus in the aged P10 MSC sheet groups was significantly larger than in P3 MSC sheet groups. Finally, a significant increase of chondro-osteoclast activity was observed in the P3 MSC sheet-grafted femur. Our data demonstrates that extensive long-term culture-induced MSC aging impaired their osteogenic ability and subsequent bony callus formation, and could be used to induce cartilaginous callus formation. |
format | Online Article Text |
id | pubmed-5554246 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-55542462017-08-15 Human Mesenchymal Stromal Cell Sheet Enhances Allograft Repair in a Mouse Model Shang, Xifu Shu, Bing Wang, Yongjun Luo, Zhengliang Wang, Guangxi Barton, Shane Morandi, Massimo Max Kevil, Christopher Dong, Yufeng Sci Rep Article To determine whether cell sheets generated with long-term passaged (P10) aging human mesenchymal stromal cells (MSCs) could be used for bone tissue regeneration as tissue engineered periosteum in a femoral allograft mouse model similar to fresh passaged (P3) young MSCs. At 3 weeks after transplantation of MSC sheets, results showed more bony callus formed between allograft and host bone ends in both young P3 MSC and aged P10 MSC sheet-wrapped groups when compared to allograft alone. At 6 weeks, while both MSC sheet-wrapped allografts showed more bony callus formation when compared to allograft alone groups, the bony callus size in aged P10 MSC sheet groups was significantly less than young P3 MSC sheet groups. Biomechanical testing confirmed that P3 MSC sheet-grafted femurs had the highest biomechanical strength in the three groups. Histology sections showed that the area of the chondriod callus in the aged P10 MSC sheet groups was significantly larger than in P3 MSC sheet groups. Finally, a significant increase of chondro-osteoclast activity was observed in the P3 MSC sheet-grafted femur. Our data demonstrates that extensive long-term culture-induced MSC aging impaired their osteogenic ability and subsequent bony callus formation, and could be used to induce cartilaginous callus formation. Nature Publishing Group UK 2017-08-11 /pmc/articles/PMC5554246/ /pubmed/28801687 http://dx.doi.org/10.1038/s41598-017-08804-2 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Shang, Xifu Shu, Bing Wang, Yongjun Luo, Zhengliang Wang, Guangxi Barton, Shane Morandi, Massimo Max Kevil, Christopher Dong, Yufeng Human Mesenchymal Stromal Cell Sheet Enhances Allograft Repair in a Mouse Model |
title | Human Mesenchymal Stromal Cell Sheet Enhances Allograft Repair in a Mouse Model |
title_full | Human Mesenchymal Stromal Cell Sheet Enhances Allograft Repair in a Mouse Model |
title_fullStr | Human Mesenchymal Stromal Cell Sheet Enhances Allograft Repair in a Mouse Model |
title_full_unstemmed | Human Mesenchymal Stromal Cell Sheet Enhances Allograft Repair in a Mouse Model |
title_short | Human Mesenchymal Stromal Cell Sheet Enhances Allograft Repair in a Mouse Model |
title_sort | human mesenchymal stromal cell sheet enhances allograft repair in a mouse model |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5554246/ https://www.ncbi.nlm.nih.gov/pubmed/28801687 http://dx.doi.org/10.1038/s41598-017-08804-2 |
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