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Two FERONIA-Like Receptor Kinases Regulate Apple Fruit Ripening by Modulating Ethylene Production

Ethylene has long been known to be a critical signal controlling the ripening of climacteric fruits; however, the signaling mechanism underlying ethylene production during fruit development is unknown. Here, we report that two FERONIA-like receptor kinases (FERLs) regulate fruit ripening by modulati...

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Autores principales: Jia, Meiru, Du, Ping, Ding, Ning, Zhang, Qing, Xing, Sinian, Wei, Lingzhi, Zhao, Yaoyao, Mao, Wenwen, Li, Jizheng, Li, Bingbing, Jia, Wensuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5554343/
https://www.ncbi.nlm.nih.gov/pubmed/28848599
http://dx.doi.org/10.3389/fpls.2017.01406
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author Jia, Meiru
Du, Ping
Ding, Ning
Zhang, Qing
Xing, Sinian
Wei, Lingzhi
Zhao, Yaoyao
Mao, Wenwen
Li, Jizheng
Li, Bingbing
Jia, Wensuo
author_facet Jia, Meiru
Du, Ping
Ding, Ning
Zhang, Qing
Xing, Sinian
Wei, Lingzhi
Zhao, Yaoyao
Mao, Wenwen
Li, Jizheng
Li, Bingbing
Jia, Wensuo
author_sort Jia, Meiru
collection PubMed
description Ethylene has long been known to be a critical signal controlling the ripening of climacteric fruits; however, the signaling mechanism underlying ethylene production during fruit development is unknown. Here, we report that two FERONIA-like receptor kinases (FERLs) regulate fruit ripening by modulating ethylene production in the climacteric fruit, apple (Malus×domestica). Bioinformatic analysis indicated that the apple genome contains 14 members of the FER family (MdFERL1–17), of these 17 FERLs, MdFERL6 was expressed at the highest level in fruit. Heterologous expression of MdFERL6 or MdFERL1, the apple homolog of Arabidopsis FER, in another climacteric fruit, tomato (Solanum lycopersicum) fruit delayed ripening and suppressed ethylene production. Overexpression and antisense expression of MdFERL6 in apple fruit calli inhibited and promoted ethylene production, respectively. Additionally, virus-induced gene silencing (VIGS) of SlFERL1, the tomato homolog of FER, promoted tomato fruit ripening and ethylene production. Both MdFERL6 and MdFERL1 physically interacted with MdSAMS (S-adenosylmethionine synthase), a key enzyme in the ethylene biosynthesis pathway. MdFERL6 was expressed at high levels during early fruit development, but dramatically declined when fruit ripening commenced, implying that MdFERL6 might limit ethylene production prior to fruit development and the ethylene production burst during fruit ripening. These results indicate that FERLs regulate apple and tomato fruit ripening, shedding light on the molecular mechanisms underlying ripening in climacteric fruit.
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spelling pubmed-55543432017-08-28 Two FERONIA-Like Receptor Kinases Regulate Apple Fruit Ripening by Modulating Ethylene Production Jia, Meiru Du, Ping Ding, Ning Zhang, Qing Xing, Sinian Wei, Lingzhi Zhao, Yaoyao Mao, Wenwen Li, Jizheng Li, Bingbing Jia, Wensuo Front Plant Sci Plant Science Ethylene has long been known to be a critical signal controlling the ripening of climacteric fruits; however, the signaling mechanism underlying ethylene production during fruit development is unknown. Here, we report that two FERONIA-like receptor kinases (FERLs) regulate fruit ripening by modulating ethylene production in the climacteric fruit, apple (Malus×domestica). Bioinformatic analysis indicated that the apple genome contains 14 members of the FER family (MdFERL1–17), of these 17 FERLs, MdFERL6 was expressed at the highest level in fruit. Heterologous expression of MdFERL6 or MdFERL1, the apple homolog of Arabidopsis FER, in another climacteric fruit, tomato (Solanum lycopersicum) fruit delayed ripening and suppressed ethylene production. Overexpression and antisense expression of MdFERL6 in apple fruit calli inhibited and promoted ethylene production, respectively. Additionally, virus-induced gene silencing (VIGS) of SlFERL1, the tomato homolog of FER, promoted tomato fruit ripening and ethylene production. Both MdFERL6 and MdFERL1 physically interacted with MdSAMS (S-adenosylmethionine synthase), a key enzyme in the ethylene biosynthesis pathway. MdFERL6 was expressed at high levels during early fruit development, but dramatically declined when fruit ripening commenced, implying that MdFERL6 might limit ethylene production prior to fruit development and the ethylene production burst during fruit ripening. These results indicate that FERLs regulate apple and tomato fruit ripening, shedding light on the molecular mechanisms underlying ripening in climacteric fruit. Frontiers Media S.A. 2017-08-10 /pmc/articles/PMC5554343/ /pubmed/28848599 http://dx.doi.org/10.3389/fpls.2017.01406 Text en Copyright © 2017 Jia, Du, Ding, Zhang, Xing, Wei, Zhao, Mao, Li, Li and Jia. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Jia, Meiru
Du, Ping
Ding, Ning
Zhang, Qing
Xing, Sinian
Wei, Lingzhi
Zhao, Yaoyao
Mao, Wenwen
Li, Jizheng
Li, Bingbing
Jia, Wensuo
Two FERONIA-Like Receptor Kinases Regulate Apple Fruit Ripening by Modulating Ethylene Production
title Two FERONIA-Like Receptor Kinases Regulate Apple Fruit Ripening by Modulating Ethylene Production
title_full Two FERONIA-Like Receptor Kinases Regulate Apple Fruit Ripening by Modulating Ethylene Production
title_fullStr Two FERONIA-Like Receptor Kinases Regulate Apple Fruit Ripening by Modulating Ethylene Production
title_full_unstemmed Two FERONIA-Like Receptor Kinases Regulate Apple Fruit Ripening by Modulating Ethylene Production
title_short Two FERONIA-Like Receptor Kinases Regulate Apple Fruit Ripening by Modulating Ethylene Production
title_sort two feronia-like receptor kinases regulate apple fruit ripening by modulating ethylene production
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5554343/
https://www.ncbi.nlm.nih.gov/pubmed/28848599
http://dx.doi.org/10.3389/fpls.2017.01406
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