Cargando…

Development of a High-Efficient Mutation Resource with Phenotypic Variation in Hexaploid Winter Wheat and Identification of Novel Alleles in the TaAGP.L-B1 Gene

Mutated genetic resources play an important role in gene/allele characterization. Currently, there are few hexaploid winter wheat mutated resources available. Here, we developed a hexaploid winter wheat resource by inducing mutations via EMS treatment by the single seed descent method. A broad mutat...

Descripción completa

Detalles Bibliográficos
Autores principales: Guo, Huijun, Yan, Zhihui, Li, Xiao, Xie, Yongdun, Xiong, Hongchun, Liu, Yunchuan, Zhao, Linshu, Gu, Jiayu, Zhao, Shirong, Liu, Luxiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5554398/
https://www.ncbi.nlm.nih.gov/pubmed/28848598
http://dx.doi.org/10.3389/fpls.2017.01404
_version_ 1783256786770329600
author Guo, Huijun
Yan, Zhihui
Li, Xiao
Xie, Yongdun
Xiong, Hongchun
Liu, Yunchuan
Zhao, Linshu
Gu, Jiayu
Zhao, Shirong
Liu, Luxiang
author_facet Guo, Huijun
Yan, Zhihui
Li, Xiao
Xie, Yongdun
Xiong, Hongchun
Liu, Yunchuan
Zhao, Linshu
Gu, Jiayu
Zhao, Shirong
Liu, Luxiang
author_sort Guo, Huijun
collection PubMed
description Mutated genetic resources play an important role in gene/allele characterization. Currently, there are few hexaploid winter wheat mutated resources available. Here, we developed a hexaploid winter wheat resource by inducing mutations via EMS treatment by the single seed descent method. A broad mutation spectrum with high mutation frequency (∼19%) on phenotypic variations was identified. These mutations included spike, leaf and seed morphology, plant architecture, and heading date variations. To evaluate the efficiency of the resource for reverse genetic analysis, allelic variations in the TaAGP.L-B1 gene, encoding the AGPase large subunit, were screened by the TILLING approach. Four missense mutations were identified and one allele in line E3-1-3, resulted in an amino acid change predicated to have severe effects on gene function. The other three mutations were predicted to have no effect. Results of gene expression patterns and grain starch content demonstrated that the novel allele in E3-1-3 altered the function of TaAGP.L-B1. Our results indicated that this mutated genetic wheat resource contained broad spectrum phenotypic and genotypic variations, that may be useful for wheat improvement, gene discovery, and functional genomics.
format Online
Article
Text
id pubmed-5554398
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-55543982017-08-28 Development of a High-Efficient Mutation Resource with Phenotypic Variation in Hexaploid Winter Wheat and Identification of Novel Alleles in the TaAGP.L-B1 Gene Guo, Huijun Yan, Zhihui Li, Xiao Xie, Yongdun Xiong, Hongchun Liu, Yunchuan Zhao, Linshu Gu, Jiayu Zhao, Shirong Liu, Luxiang Front Plant Sci Plant Science Mutated genetic resources play an important role in gene/allele characterization. Currently, there are few hexaploid winter wheat mutated resources available. Here, we developed a hexaploid winter wheat resource by inducing mutations via EMS treatment by the single seed descent method. A broad mutation spectrum with high mutation frequency (∼19%) on phenotypic variations was identified. These mutations included spike, leaf and seed morphology, plant architecture, and heading date variations. To evaluate the efficiency of the resource for reverse genetic analysis, allelic variations in the TaAGP.L-B1 gene, encoding the AGPase large subunit, were screened by the TILLING approach. Four missense mutations were identified and one allele in line E3-1-3, resulted in an amino acid change predicated to have severe effects on gene function. The other three mutations were predicted to have no effect. Results of gene expression patterns and grain starch content demonstrated that the novel allele in E3-1-3 altered the function of TaAGP.L-B1. Our results indicated that this mutated genetic wheat resource contained broad spectrum phenotypic and genotypic variations, that may be useful for wheat improvement, gene discovery, and functional genomics. Frontiers Media S.A. 2017-08-10 /pmc/articles/PMC5554398/ /pubmed/28848598 http://dx.doi.org/10.3389/fpls.2017.01404 Text en Copyright © 2017 Guo, Yan, Li, Xie, Xiong, Liu, Zhao, Gu, Zhao and Liu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Guo, Huijun
Yan, Zhihui
Li, Xiao
Xie, Yongdun
Xiong, Hongchun
Liu, Yunchuan
Zhao, Linshu
Gu, Jiayu
Zhao, Shirong
Liu, Luxiang
Development of a High-Efficient Mutation Resource with Phenotypic Variation in Hexaploid Winter Wheat and Identification of Novel Alleles in the TaAGP.L-B1 Gene
title Development of a High-Efficient Mutation Resource with Phenotypic Variation in Hexaploid Winter Wheat and Identification of Novel Alleles in the TaAGP.L-B1 Gene
title_full Development of a High-Efficient Mutation Resource with Phenotypic Variation in Hexaploid Winter Wheat and Identification of Novel Alleles in the TaAGP.L-B1 Gene
title_fullStr Development of a High-Efficient Mutation Resource with Phenotypic Variation in Hexaploid Winter Wheat and Identification of Novel Alleles in the TaAGP.L-B1 Gene
title_full_unstemmed Development of a High-Efficient Mutation Resource with Phenotypic Variation in Hexaploid Winter Wheat and Identification of Novel Alleles in the TaAGP.L-B1 Gene
title_short Development of a High-Efficient Mutation Resource with Phenotypic Variation in Hexaploid Winter Wheat and Identification of Novel Alleles in the TaAGP.L-B1 Gene
title_sort development of a high-efficient mutation resource with phenotypic variation in hexaploid winter wheat and identification of novel alleles in the taagp.l-b1 gene
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5554398/
https://www.ncbi.nlm.nih.gov/pubmed/28848598
http://dx.doi.org/10.3389/fpls.2017.01404
work_keys_str_mv AT guohuijun developmentofahighefficientmutationresourcewithphenotypicvariationinhexaploidwinterwheatandidentificationofnovelallelesinthetaagplb1gene
AT yanzhihui developmentofahighefficientmutationresourcewithphenotypicvariationinhexaploidwinterwheatandidentificationofnovelallelesinthetaagplb1gene
AT lixiao developmentofahighefficientmutationresourcewithphenotypicvariationinhexaploidwinterwheatandidentificationofnovelallelesinthetaagplb1gene
AT xieyongdun developmentofahighefficientmutationresourcewithphenotypicvariationinhexaploidwinterwheatandidentificationofnovelallelesinthetaagplb1gene
AT xionghongchun developmentofahighefficientmutationresourcewithphenotypicvariationinhexaploidwinterwheatandidentificationofnovelallelesinthetaagplb1gene
AT liuyunchuan developmentofahighefficientmutationresourcewithphenotypicvariationinhexaploidwinterwheatandidentificationofnovelallelesinthetaagplb1gene
AT zhaolinshu developmentofahighefficientmutationresourcewithphenotypicvariationinhexaploidwinterwheatandidentificationofnovelallelesinthetaagplb1gene
AT gujiayu developmentofahighefficientmutationresourcewithphenotypicvariationinhexaploidwinterwheatandidentificationofnovelallelesinthetaagplb1gene
AT zhaoshirong developmentofahighefficientmutationresourcewithphenotypicvariationinhexaploidwinterwheatandidentificationofnovelallelesinthetaagplb1gene
AT liuluxiang developmentofahighefficientmutationresourcewithphenotypicvariationinhexaploidwinterwheatandidentificationofnovelallelesinthetaagplb1gene