Cargando…
Acetylation of hMOF Modulates H4K16ac to Regulate DNA Repair Genes in Response to Oxidative Stress
Oxidative stress is considered to be a key risk state for a variety of human diseases. In response to oxidative stress, the regulation of transcriptional expression of DNA repair genes would be important to DNA repair and genomic stability. However, the overall pattern of transcriptional expression...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Ivyspring International Publisher
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5555109/ https://www.ncbi.nlm.nih.gov/pubmed/28808424 http://dx.doi.org/10.7150/ijbs.17260 |
_version_ | 1783256887823695872 |
---|---|
author | Zhong, Jianing Ji, Liying Chen, Huiqian Li, Xianfeng Zhang, Jian'an Wang, Xingxing Wu, Weilin Xu, Ying Huang, Fei Cai, Wanshi Sun, Zhong Sheng |
author_facet | Zhong, Jianing Ji, Liying Chen, Huiqian Li, Xianfeng Zhang, Jian'an Wang, Xingxing Wu, Weilin Xu, Ying Huang, Fei Cai, Wanshi Sun, Zhong Sheng |
author_sort | Zhong, Jianing |
collection | PubMed |
description | Oxidative stress is considered to be a key risk state for a variety of human diseases. In response to oxidative stress, the regulation of transcriptional expression of DNA repair genes would be important to DNA repair and genomic stability. However, the overall pattern of transcriptional expression of DNA repair genes and the underlying molecular response mechanism to oxidative stress remain unclear. Here, by employing colorectal cancer cell lines following exposure to hydrogen peroxide, we generated expression profiles of DNA repair genes via RNA-seq and identified gene subsets that are induced or repressed following oxidative stress exposure. RRBS-seq analyses further indicated that transcriptional regulation of most of the DNA repair genes that were induced or repressed is independent of their DNA methylation status. Our analyses also indicate that hydrogen peroxide induces deacetylase SIRT1 which decreases chromatin affinity and the activity of histone acetyltransferase hMOF toward H4K16ac and results in decreased transcriptional expression of DNA repair genes. Taken together, our findings provide a potential mechanism by which oxidative stress suppresses DNA repair genes which is independent of the DNA methylation status of their promoters. |
format | Online Article Text |
id | pubmed-5555109 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-55551092017-08-14 Acetylation of hMOF Modulates H4K16ac to Regulate DNA Repair Genes in Response to Oxidative Stress Zhong, Jianing Ji, Liying Chen, Huiqian Li, Xianfeng Zhang, Jian'an Wang, Xingxing Wu, Weilin Xu, Ying Huang, Fei Cai, Wanshi Sun, Zhong Sheng Int J Biol Sci Research Paper Oxidative stress is considered to be a key risk state for a variety of human diseases. In response to oxidative stress, the regulation of transcriptional expression of DNA repair genes would be important to DNA repair and genomic stability. However, the overall pattern of transcriptional expression of DNA repair genes and the underlying molecular response mechanism to oxidative stress remain unclear. Here, by employing colorectal cancer cell lines following exposure to hydrogen peroxide, we generated expression profiles of DNA repair genes via RNA-seq and identified gene subsets that are induced or repressed following oxidative stress exposure. RRBS-seq analyses further indicated that transcriptional regulation of most of the DNA repair genes that were induced or repressed is independent of their DNA methylation status. Our analyses also indicate that hydrogen peroxide induces deacetylase SIRT1 which decreases chromatin affinity and the activity of histone acetyltransferase hMOF toward H4K16ac and results in decreased transcriptional expression of DNA repair genes. Taken together, our findings provide a potential mechanism by which oxidative stress suppresses DNA repair genes which is independent of the DNA methylation status of their promoters. Ivyspring International Publisher 2017-07-15 /pmc/articles/PMC5555109/ /pubmed/28808424 http://dx.doi.org/10.7150/ijbs.17260 Text en © Ivyspring International Publisher This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/). See http://ivyspring.com/terms for full terms and conditions. |
spellingShingle | Research Paper Zhong, Jianing Ji, Liying Chen, Huiqian Li, Xianfeng Zhang, Jian'an Wang, Xingxing Wu, Weilin Xu, Ying Huang, Fei Cai, Wanshi Sun, Zhong Sheng Acetylation of hMOF Modulates H4K16ac to Regulate DNA Repair Genes in Response to Oxidative Stress |
title | Acetylation of hMOF Modulates H4K16ac to Regulate DNA Repair Genes in Response to Oxidative Stress |
title_full | Acetylation of hMOF Modulates H4K16ac to Regulate DNA Repair Genes in Response to Oxidative Stress |
title_fullStr | Acetylation of hMOF Modulates H4K16ac to Regulate DNA Repair Genes in Response to Oxidative Stress |
title_full_unstemmed | Acetylation of hMOF Modulates H4K16ac to Regulate DNA Repair Genes in Response to Oxidative Stress |
title_short | Acetylation of hMOF Modulates H4K16ac to Regulate DNA Repair Genes in Response to Oxidative Stress |
title_sort | acetylation of hmof modulates h4k16ac to regulate dna repair genes in response to oxidative stress |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5555109/ https://www.ncbi.nlm.nih.gov/pubmed/28808424 http://dx.doi.org/10.7150/ijbs.17260 |
work_keys_str_mv | AT zhongjianing acetylationofhmofmodulatesh4k16actoregulatednarepairgenesinresponsetooxidativestress AT jiliying acetylationofhmofmodulatesh4k16actoregulatednarepairgenesinresponsetooxidativestress AT chenhuiqian acetylationofhmofmodulatesh4k16actoregulatednarepairgenesinresponsetooxidativestress AT lixianfeng acetylationofhmofmodulatesh4k16actoregulatednarepairgenesinresponsetooxidativestress AT zhangjianan acetylationofhmofmodulatesh4k16actoregulatednarepairgenesinresponsetooxidativestress AT wangxingxing acetylationofhmofmodulatesh4k16actoregulatednarepairgenesinresponsetooxidativestress AT wuweilin acetylationofhmofmodulatesh4k16actoregulatednarepairgenesinresponsetooxidativestress AT xuying acetylationofhmofmodulatesh4k16actoregulatednarepairgenesinresponsetooxidativestress AT huangfei acetylationofhmofmodulatesh4k16actoregulatednarepairgenesinresponsetooxidativestress AT caiwanshi acetylationofhmofmodulatesh4k16actoregulatednarepairgenesinresponsetooxidativestress AT sunzhongsheng acetylationofhmofmodulatesh4k16actoregulatednarepairgenesinresponsetooxidativestress |