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Characterizing the Pyrenophora teres f. maculata–Barley Interaction Using Pathogen Genetics
Pyrenophora teres f. maculata is the cause of the foliar disease spot form net blotch (SFNB) on barley. To evaluate pathogen genetics underlying the P. teres f. maculata–barley interaction, we developed a 105-progeny population by crossing two globally diverse isolates, one from North Dakota and the...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Genetics Society of America
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5555467/ https://www.ncbi.nlm.nih.gov/pubmed/28659291 http://dx.doi.org/10.1534/g3.117.043265 |
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author | Carlsen, Steven A. Neupane, Anjan Wyatt, Nathan A. Richards, Jonathan K. Faris, Justin D. Xu, Steven S. Brueggeman, Robert S. Friesen, Timothy L. |
author_facet | Carlsen, Steven A. Neupane, Anjan Wyatt, Nathan A. Richards, Jonathan K. Faris, Justin D. Xu, Steven S. Brueggeman, Robert S. Friesen, Timothy L. |
author_sort | Carlsen, Steven A. |
collection | PubMed |
description | Pyrenophora teres f. maculata is the cause of the foliar disease spot form net blotch (SFNB) on barley. To evaluate pathogen genetics underlying the P. teres f. maculata–barley interaction, we developed a 105-progeny population by crossing two globally diverse isolates, one from North Dakota and the other from Western Australia. Progeny were phenotyped on a set of four barley genotypes showing a differential reaction to the parental isolates, then genotyped using a restriction site-associated-genotype-by-sequencing (RAD-GBS) approach. Genetic maps were developed for use in quantitative trait locus (QTL) analysis to identify virulence-associated QTL. Six QTL were identified on five different linkage groups and individually accounted for 20–37% of the disease variation, with the number of significant QTL ranging from two to four for the barley genotypes evaluated. The data presented demonstrate the complexity of virulence involved in the P. teres f. maculata–barley pathosystem and begins to lay the foundation for understanding this important interaction. |
format | Online Article Text |
id | pubmed-5555467 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Genetics Society of America |
record_format | MEDLINE/PubMed |
spelling | pubmed-55554672017-08-17 Characterizing the Pyrenophora teres f. maculata–Barley Interaction Using Pathogen Genetics Carlsen, Steven A. Neupane, Anjan Wyatt, Nathan A. Richards, Jonathan K. Faris, Justin D. Xu, Steven S. Brueggeman, Robert S. Friesen, Timothy L. G3 (Bethesda) Investigations Pyrenophora teres f. maculata is the cause of the foliar disease spot form net blotch (SFNB) on barley. To evaluate pathogen genetics underlying the P. teres f. maculata–barley interaction, we developed a 105-progeny population by crossing two globally diverse isolates, one from North Dakota and the other from Western Australia. Progeny were phenotyped on a set of four barley genotypes showing a differential reaction to the parental isolates, then genotyped using a restriction site-associated-genotype-by-sequencing (RAD-GBS) approach. Genetic maps were developed for use in quantitative trait locus (QTL) analysis to identify virulence-associated QTL. Six QTL were identified on five different linkage groups and individually accounted for 20–37% of the disease variation, with the number of significant QTL ranging from two to four for the barley genotypes evaluated. The data presented demonstrate the complexity of virulence involved in the P. teres f. maculata–barley pathosystem and begins to lay the foundation for understanding this important interaction. Genetics Society of America 2017-06-28 /pmc/articles/PMC5555467/ /pubmed/28659291 http://dx.doi.org/10.1534/g3.117.043265 Text en Copyright © 2017 Carlsen et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Investigations Carlsen, Steven A. Neupane, Anjan Wyatt, Nathan A. Richards, Jonathan K. Faris, Justin D. Xu, Steven S. Brueggeman, Robert S. Friesen, Timothy L. Characterizing the Pyrenophora teres f. maculata–Barley Interaction Using Pathogen Genetics |
title | Characterizing the Pyrenophora teres f. maculata–Barley Interaction Using Pathogen Genetics |
title_full | Characterizing the Pyrenophora teres f. maculata–Barley Interaction Using Pathogen Genetics |
title_fullStr | Characterizing the Pyrenophora teres f. maculata–Barley Interaction Using Pathogen Genetics |
title_full_unstemmed | Characterizing the Pyrenophora teres f. maculata–Barley Interaction Using Pathogen Genetics |
title_short | Characterizing the Pyrenophora teres f. maculata–Barley Interaction Using Pathogen Genetics |
title_sort | characterizing the pyrenophora teres f. maculata–barley interaction using pathogen genetics |
topic | Investigations |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5555467/ https://www.ncbi.nlm.nih.gov/pubmed/28659291 http://dx.doi.org/10.1534/g3.117.043265 |
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