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Transcriptional Profiling of Biofilm Regulators Identified by an Overexpression Screen in Saccharomyces cerevisiae

Biofilm formation by microorganisms is a major cause of recurring infections and removal of biofilms has proven to be extremely difficult given their inherent drug resistance . Understanding the biological processes that underlie biofilm formation is thus extremely important and could lead to the de...

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Autores principales: Cromie, Gareth A., Tan, Zhihao, Hays, Michelle, Sirr, Amy, Jeffery, Eric W., Dudley, Aimée M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Genetics Society of America 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5555487/
https://www.ncbi.nlm.nih.gov/pubmed/28673928
http://dx.doi.org/10.1534/g3.117.042440
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author Cromie, Gareth A.
Tan, Zhihao
Hays, Michelle
Sirr, Amy
Jeffery, Eric W.
Dudley, Aimée M.
author_facet Cromie, Gareth A.
Tan, Zhihao
Hays, Michelle
Sirr, Amy
Jeffery, Eric W.
Dudley, Aimée M.
author_sort Cromie, Gareth A.
collection PubMed
description Biofilm formation by microorganisms is a major cause of recurring infections and removal of biofilms has proven to be extremely difficult given their inherent drug resistance . Understanding the biological processes that underlie biofilm formation is thus extremely important and could lead to the development of more effective drug therapies, resulting in better infection outcomes. Using the yeast Saccharomyces cerevisiae as a biofilm model, overexpression screens identified DIG1, SFL1, HEK2, TOS8, SAN1, and ROF1/YHR177W as regulators of biofilm formation. Subsequent RNA-seq analysis of biofilm and nonbiofilm-forming strains revealed that all of the overexpression strains, other than DIG1 and TOS8, were adopting a single differential expression profile, although induced to varying degrees. TOS8 adopted a separate profile, while the expression profile of DIG1 reflected the common pattern seen in most of the strains, plus substantial DIG1-specific expression changes. We interpret the existence of the common transcriptional pattern seen across multiple, unrelated overexpression strains as reflecting a transcriptional state, that the yeast cell can access through regulatory signaling mechanisms, allowing an adaptive morphological change between biofilm-forming and nonbiofilm states.
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spelling pubmed-55554872017-08-17 Transcriptional Profiling of Biofilm Regulators Identified by an Overexpression Screen in Saccharomyces cerevisiae Cromie, Gareth A. Tan, Zhihao Hays, Michelle Sirr, Amy Jeffery, Eric W. Dudley, Aimée M. G3 (Bethesda) Investigations Biofilm formation by microorganisms is a major cause of recurring infections and removal of biofilms has proven to be extremely difficult given their inherent drug resistance . Understanding the biological processes that underlie biofilm formation is thus extremely important and could lead to the development of more effective drug therapies, resulting in better infection outcomes. Using the yeast Saccharomyces cerevisiae as a biofilm model, overexpression screens identified DIG1, SFL1, HEK2, TOS8, SAN1, and ROF1/YHR177W as regulators of biofilm formation. Subsequent RNA-seq analysis of biofilm and nonbiofilm-forming strains revealed that all of the overexpression strains, other than DIG1 and TOS8, were adopting a single differential expression profile, although induced to varying degrees. TOS8 adopted a separate profile, while the expression profile of DIG1 reflected the common pattern seen in most of the strains, plus substantial DIG1-specific expression changes. We interpret the existence of the common transcriptional pattern seen across multiple, unrelated overexpression strains as reflecting a transcriptional state, that the yeast cell can access through regulatory signaling mechanisms, allowing an adaptive morphological change between biofilm-forming and nonbiofilm states. Genetics Society of America 2017-07-03 /pmc/articles/PMC5555487/ /pubmed/28673928 http://dx.doi.org/10.1534/g3.117.042440 Text en Copyright © 2017 Cromie et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Investigations
Cromie, Gareth A.
Tan, Zhihao
Hays, Michelle
Sirr, Amy
Jeffery, Eric W.
Dudley, Aimée M.
Transcriptional Profiling of Biofilm Regulators Identified by an Overexpression Screen in Saccharomyces cerevisiae
title Transcriptional Profiling of Biofilm Regulators Identified by an Overexpression Screen in Saccharomyces cerevisiae
title_full Transcriptional Profiling of Biofilm Regulators Identified by an Overexpression Screen in Saccharomyces cerevisiae
title_fullStr Transcriptional Profiling of Biofilm Regulators Identified by an Overexpression Screen in Saccharomyces cerevisiae
title_full_unstemmed Transcriptional Profiling of Biofilm Regulators Identified by an Overexpression Screen in Saccharomyces cerevisiae
title_short Transcriptional Profiling of Biofilm Regulators Identified by an Overexpression Screen in Saccharomyces cerevisiae
title_sort transcriptional profiling of biofilm regulators identified by an overexpression screen in saccharomyces cerevisiae
topic Investigations
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5555487/
https://www.ncbi.nlm.nih.gov/pubmed/28673928
http://dx.doi.org/10.1534/g3.117.042440
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