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Ikk2 regulates cytokinesis during vertebrate development
NFκB signaling has a pivotal role in regulation of development, innate immunity, and inflammation. Ikk2 is one of the two critical kinases that regulate the NFκB signaling pathway. While the role of Ikk2 in immunity, inflammation and oncogenesis has received attention, an understanding of the role o...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5556003/ https://www.ncbi.nlm.nih.gov/pubmed/28808254 http://dx.doi.org/10.1038/s41598-017-06904-7 |
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author | Shen, Hongyuan Shin, Eun Myoung Lee, Serene Mathavan, Sinnakaruppan Koh, Hiromi Osato, Motomi Choi, Hyungwon Tergaonkar, Vinay Korzh, Vladimir |
author_facet | Shen, Hongyuan Shin, Eun Myoung Lee, Serene Mathavan, Sinnakaruppan Koh, Hiromi Osato, Motomi Choi, Hyungwon Tergaonkar, Vinay Korzh, Vladimir |
author_sort | Shen, Hongyuan |
collection | PubMed |
description | NFκB signaling has a pivotal role in regulation of development, innate immunity, and inflammation. Ikk2 is one of the two critical kinases that regulate the NFκB signaling pathway. While the role of Ikk2 in immunity, inflammation and oncogenesis has received attention, an understanding of the role of Ikk2 in vertebrate development has been compounded by the embryonic lethality seen in mice lacking Ikk2. We find that despite abnormal angiogenesis in IKK2 zygotic mutants of zebrafish, the maternal activity of Ikk2 supports embryogenesis and maturation of fertile animals and allows to study the role of IKK2 in development. Maternal-zygotic ikk2 mutants represent the first vertebrates globally devoid of maternal and zygotic Ikk2 activity. They are defective in cell proliferation as evidenced by abnormal cytokinesis, nuclear enlargement and syncytialisation of a significant portion of blastoderm. We further document that reduced phosphorylation of Aurora A by Ikk2 could underlie the basis of these defects in cell division. |
format | Online Article Text |
id | pubmed-5556003 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-55560032017-08-16 Ikk2 regulates cytokinesis during vertebrate development Shen, Hongyuan Shin, Eun Myoung Lee, Serene Mathavan, Sinnakaruppan Koh, Hiromi Osato, Motomi Choi, Hyungwon Tergaonkar, Vinay Korzh, Vladimir Sci Rep Article NFκB signaling has a pivotal role in regulation of development, innate immunity, and inflammation. Ikk2 is one of the two critical kinases that regulate the NFκB signaling pathway. While the role of Ikk2 in immunity, inflammation and oncogenesis has received attention, an understanding of the role of Ikk2 in vertebrate development has been compounded by the embryonic lethality seen in mice lacking Ikk2. We find that despite abnormal angiogenesis in IKK2 zygotic mutants of zebrafish, the maternal activity of Ikk2 supports embryogenesis and maturation of fertile animals and allows to study the role of IKK2 in development. Maternal-zygotic ikk2 mutants represent the first vertebrates globally devoid of maternal and zygotic Ikk2 activity. They are defective in cell proliferation as evidenced by abnormal cytokinesis, nuclear enlargement and syncytialisation of a significant portion of blastoderm. We further document that reduced phosphorylation of Aurora A by Ikk2 could underlie the basis of these defects in cell division. Nature Publishing Group UK 2017-08-14 /pmc/articles/PMC5556003/ /pubmed/28808254 http://dx.doi.org/10.1038/s41598-017-06904-7 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Shen, Hongyuan Shin, Eun Myoung Lee, Serene Mathavan, Sinnakaruppan Koh, Hiromi Osato, Motomi Choi, Hyungwon Tergaonkar, Vinay Korzh, Vladimir Ikk2 regulates cytokinesis during vertebrate development |
title | Ikk2 regulates cytokinesis during vertebrate development |
title_full | Ikk2 regulates cytokinesis during vertebrate development |
title_fullStr | Ikk2 regulates cytokinesis during vertebrate development |
title_full_unstemmed | Ikk2 regulates cytokinesis during vertebrate development |
title_short | Ikk2 regulates cytokinesis during vertebrate development |
title_sort | ikk2 regulates cytokinesis during vertebrate development |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5556003/ https://www.ncbi.nlm.nih.gov/pubmed/28808254 http://dx.doi.org/10.1038/s41598-017-06904-7 |
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