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Ikk2 regulates cytokinesis during vertebrate development

NFκB signaling has a pivotal role in regulation of development, innate immunity, and inflammation. Ikk2 is one of the two critical kinases that regulate the NFκB signaling pathway. While the role of Ikk2 in immunity, inflammation and oncogenesis has received attention, an understanding of the role o...

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Autores principales: Shen, Hongyuan, Shin, Eun Myoung, Lee, Serene, Mathavan, Sinnakaruppan, Koh, Hiromi, Osato, Motomi, Choi, Hyungwon, Tergaonkar, Vinay, Korzh, Vladimir
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5556003/
https://www.ncbi.nlm.nih.gov/pubmed/28808254
http://dx.doi.org/10.1038/s41598-017-06904-7
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author Shen, Hongyuan
Shin, Eun Myoung
Lee, Serene
Mathavan, Sinnakaruppan
Koh, Hiromi
Osato, Motomi
Choi, Hyungwon
Tergaonkar, Vinay
Korzh, Vladimir
author_facet Shen, Hongyuan
Shin, Eun Myoung
Lee, Serene
Mathavan, Sinnakaruppan
Koh, Hiromi
Osato, Motomi
Choi, Hyungwon
Tergaonkar, Vinay
Korzh, Vladimir
author_sort Shen, Hongyuan
collection PubMed
description NFκB signaling has a pivotal role in regulation of development, innate immunity, and inflammation. Ikk2 is one of the two critical kinases that regulate the NFκB signaling pathway. While the role of Ikk2 in immunity, inflammation and oncogenesis has received attention, an understanding of the role of Ikk2 in vertebrate development has been compounded by the embryonic lethality seen in mice lacking Ikk2. We find that despite abnormal angiogenesis in IKK2 zygotic mutants of zebrafish, the maternal activity of Ikk2 supports embryogenesis and maturation of fertile animals and allows to study the role of IKK2 in development. Maternal-zygotic ikk2 mutants represent the first vertebrates globally devoid of maternal and zygotic Ikk2 activity. They are defective in cell proliferation as evidenced by abnormal cytokinesis, nuclear enlargement and syncytialisation of a significant portion of blastoderm. We further document that reduced phosphorylation of Aurora A by Ikk2 could underlie the basis of these defects in cell division.
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spelling pubmed-55560032017-08-16 Ikk2 regulates cytokinesis during vertebrate development Shen, Hongyuan Shin, Eun Myoung Lee, Serene Mathavan, Sinnakaruppan Koh, Hiromi Osato, Motomi Choi, Hyungwon Tergaonkar, Vinay Korzh, Vladimir Sci Rep Article NFκB signaling has a pivotal role in regulation of development, innate immunity, and inflammation. Ikk2 is one of the two critical kinases that regulate the NFκB signaling pathway. While the role of Ikk2 in immunity, inflammation and oncogenesis has received attention, an understanding of the role of Ikk2 in vertebrate development has been compounded by the embryonic lethality seen in mice lacking Ikk2. We find that despite abnormal angiogenesis in IKK2 zygotic mutants of zebrafish, the maternal activity of Ikk2 supports embryogenesis and maturation of fertile animals and allows to study the role of IKK2 in development. Maternal-zygotic ikk2 mutants represent the first vertebrates globally devoid of maternal and zygotic Ikk2 activity. They are defective in cell proliferation as evidenced by abnormal cytokinesis, nuclear enlargement and syncytialisation of a significant portion of blastoderm. We further document that reduced phosphorylation of Aurora A by Ikk2 could underlie the basis of these defects in cell division. Nature Publishing Group UK 2017-08-14 /pmc/articles/PMC5556003/ /pubmed/28808254 http://dx.doi.org/10.1038/s41598-017-06904-7 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Shen, Hongyuan
Shin, Eun Myoung
Lee, Serene
Mathavan, Sinnakaruppan
Koh, Hiromi
Osato, Motomi
Choi, Hyungwon
Tergaonkar, Vinay
Korzh, Vladimir
Ikk2 regulates cytokinesis during vertebrate development
title Ikk2 regulates cytokinesis during vertebrate development
title_full Ikk2 regulates cytokinesis during vertebrate development
title_fullStr Ikk2 regulates cytokinesis during vertebrate development
title_full_unstemmed Ikk2 regulates cytokinesis during vertebrate development
title_short Ikk2 regulates cytokinesis during vertebrate development
title_sort ikk2 regulates cytokinesis during vertebrate development
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5556003/
https://www.ncbi.nlm.nih.gov/pubmed/28808254
http://dx.doi.org/10.1038/s41598-017-06904-7
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