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Modular Serial Flow Through device for pulsed electric field treatment of the liquid samples
In biotechnology, medicine, and food processing, simple and reliable methods for cell membrane permeabilization are required for drug/gene delivery into the cells or for the inactivation of undesired microorganisms. Pulsed electric field treatment is among the most promising methods enabling both ai...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5556104/ https://www.ncbi.nlm.nih.gov/pubmed/28808315 http://dx.doi.org/10.1038/s41598-017-08620-8 |
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author | Kandušer, Maša Belič, Aleš Čorović, Selma Škrjanc, Igor |
author_facet | Kandušer, Maša Belič, Aleš Čorović, Selma Škrjanc, Igor |
author_sort | Kandušer, Maša |
collection | PubMed |
description | In biotechnology, medicine, and food processing, simple and reliable methods for cell membrane permeabilization are required for drug/gene delivery into the cells or for the inactivation of undesired microorganisms. Pulsed electric field treatment is among the most promising methods enabling both aims. The drawback in current technology is controllable large volume operation. To address this challenge, we have developed an experimental setup for flow through electroporation with online regulation of the flow rate with feedback control. We have designed a modular serial flow-through co-linear chamber with a smooth inner surface, the uniform cross-section geometry through the majority of the system’s length, and the mesh in contact with the electrodes, which provides uniform electric field distribution and fluid velocity equilibration. The cylindrical cross-section of the chamber prevents arching at the active treatment region. We used mathematical modeling for the evaluation of electric field distribution and the flow profile in the active region. The system was tested for the inactivation of Escherichia coli. We compared two flow-through chambers and used a static chamber as a reference. The experiments were performed under identical experimental condition (product and similar process parameters). The data were analyzed in terms of inactivation efficiency and specific energy consumption. |
format | Online Article Text |
id | pubmed-5556104 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-55561042017-08-16 Modular Serial Flow Through device for pulsed electric field treatment of the liquid samples Kandušer, Maša Belič, Aleš Čorović, Selma Škrjanc, Igor Sci Rep Article In biotechnology, medicine, and food processing, simple and reliable methods for cell membrane permeabilization are required for drug/gene delivery into the cells or for the inactivation of undesired microorganisms. Pulsed electric field treatment is among the most promising methods enabling both aims. The drawback in current technology is controllable large volume operation. To address this challenge, we have developed an experimental setup for flow through electroporation with online regulation of the flow rate with feedback control. We have designed a modular serial flow-through co-linear chamber with a smooth inner surface, the uniform cross-section geometry through the majority of the system’s length, and the mesh in contact with the electrodes, which provides uniform electric field distribution and fluid velocity equilibration. The cylindrical cross-section of the chamber prevents arching at the active treatment region. We used mathematical modeling for the evaluation of electric field distribution and the flow profile in the active region. The system was tested for the inactivation of Escherichia coli. We compared two flow-through chambers and used a static chamber as a reference. The experiments were performed under identical experimental condition (product and similar process parameters). The data were analyzed in terms of inactivation efficiency and specific energy consumption. Nature Publishing Group UK 2017-08-14 /pmc/articles/PMC5556104/ /pubmed/28808315 http://dx.doi.org/10.1038/s41598-017-08620-8 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Kandušer, Maša Belič, Aleš Čorović, Selma Škrjanc, Igor Modular Serial Flow Through device for pulsed electric field treatment of the liquid samples |
title | Modular Serial Flow Through device for pulsed electric field treatment of the liquid samples |
title_full | Modular Serial Flow Through device for pulsed electric field treatment of the liquid samples |
title_fullStr | Modular Serial Flow Through device for pulsed electric field treatment of the liquid samples |
title_full_unstemmed | Modular Serial Flow Through device for pulsed electric field treatment of the liquid samples |
title_short | Modular Serial Flow Through device for pulsed electric field treatment of the liquid samples |
title_sort | modular serial flow through device for pulsed electric field treatment of the liquid samples |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5556104/ https://www.ncbi.nlm.nih.gov/pubmed/28808315 http://dx.doi.org/10.1038/s41598-017-08620-8 |
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