Light Guided In-vivo Activation of Innate Immune Cells with Photocaged TLR 2/6 Agonist

The complexity of the immune system creates challenges in exploring its importance and robustness. To date, there have been few techniques developed to manipulate individual components of the immune system in an in vivo environment. Here we show a light-based dendritic cell (DC) activation allowing...

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Autores principales: Ryu, Keun Ah, McGonnigal, Bethany, Moore, Troy, Kargupta, Tawnya, Mancini, Rock J., Esser-Kahn, Aaron P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5556111/
https://www.ncbi.nlm.nih.gov/pubmed/28808328
http://dx.doi.org/10.1038/s41598-017-08520-x
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author Ryu, Keun Ah
McGonnigal, Bethany
Moore, Troy
Kargupta, Tawnya
Mancini, Rock J.
Esser-Kahn, Aaron P.
author_facet Ryu, Keun Ah
McGonnigal, Bethany
Moore, Troy
Kargupta, Tawnya
Mancini, Rock J.
Esser-Kahn, Aaron P.
author_sort Ryu, Keun Ah
collection PubMed
description The complexity of the immune system creates challenges in exploring its importance and robustness. To date, there have been few techniques developed to manipulate individual components of the immune system in an in vivo environment. Here we show a light-based dendritic cell (DC) activation allowing spatial and temporal control of immune activation in vivo. Additionally, we show time dependent changes in RNA profiles of the draining lymph node, suggesting a change in cell profile following DC migration and indicating that the cells migrating have been activated towards antigen presentation.
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spelling pubmed-55561112017-08-16 Light Guided In-vivo Activation of Innate Immune Cells with Photocaged TLR 2/6 Agonist Ryu, Keun Ah McGonnigal, Bethany Moore, Troy Kargupta, Tawnya Mancini, Rock J. Esser-Kahn, Aaron P. Sci Rep Article The complexity of the immune system creates challenges in exploring its importance and robustness. To date, there have been few techniques developed to manipulate individual components of the immune system in an in vivo environment. Here we show a light-based dendritic cell (DC) activation allowing spatial and temporal control of immune activation in vivo. Additionally, we show time dependent changes in RNA profiles of the draining lymph node, suggesting a change in cell profile following DC migration and indicating that the cells migrating have been activated towards antigen presentation. Nature Publishing Group UK 2017-08-14 /pmc/articles/PMC5556111/ /pubmed/28808328 http://dx.doi.org/10.1038/s41598-017-08520-x Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Ryu, Keun Ah
McGonnigal, Bethany
Moore, Troy
Kargupta, Tawnya
Mancini, Rock J.
Esser-Kahn, Aaron P.
Light Guided In-vivo Activation of Innate Immune Cells with Photocaged TLR 2/6 Agonist
title Light Guided In-vivo Activation of Innate Immune Cells with Photocaged TLR 2/6 Agonist
title_full Light Guided In-vivo Activation of Innate Immune Cells with Photocaged TLR 2/6 Agonist
title_fullStr Light Guided In-vivo Activation of Innate Immune Cells with Photocaged TLR 2/6 Agonist
title_full_unstemmed Light Guided In-vivo Activation of Innate Immune Cells with Photocaged TLR 2/6 Agonist
title_short Light Guided In-vivo Activation of Innate Immune Cells with Photocaged TLR 2/6 Agonist
title_sort light guided in-vivo activation of innate immune cells with photocaged tlr 2/6 agonist
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5556111/
https://www.ncbi.nlm.nih.gov/pubmed/28808328
http://dx.doi.org/10.1038/s41598-017-08520-x
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