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Incidence and molecular characterization of the extended spectrum beta lactamase-producing Escherichia coli isolated from urinary tract infections in Eastern Saudi Arabia

OBJECTIVES: To find the prevalence of uropathogenic Escherichia coli (E. coli)-producing extended spectrum beta lactamase (ESBL) at King Fahd Military Medical Complex in Dhahran (KFMMC) and to detect the genes responsible for its production. In addition, we determined the pattern of multi-drug resis...

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Autores principales: Mashwal, Fahad A., Safi, Salah H. El, George, Siju K., Adam, Ahmed A., Jebakumar, Arulanantham Z.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Saudi Medical Journal 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5556297/
https://www.ncbi.nlm.nih.gov/pubmed/28762433
http://dx.doi.org/10.15537/smj.2017.8.18578
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author Mashwal, Fahad A.
Safi, Salah H. El
George, Siju K.
Adam, Ahmed A.
Jebakumar, Arulanantham Z.
author_facet Mashwal, Fahad A.
Safi, Salah H. El
George, Siju K.
Adam, Ahmed A.
Jebakumar, Arulanantham Z.
author_sort Mashwal, Fahad A.
collection PubMed
description OBJECTIVES: To find the prevalence of uropathogenic Escherichia coli (E. coli)-producing extended spectrum beta lactamase (ESBL) at King Fahd Military Medical Complex in Dhahran (KFMMC) and to detect the genes responsible for its production. In addition, we determined the pattern of multi-drug resistance among isolates. METHODS: A total of 117 uropathogenic E. coli isolates were collected from KFMMC over a period of 4 months from March 2014 to June 2014. These were received in the Microbiology Laboratory at Prince Sultan Military College of Health Sciences (PSMCHS), Dhahran, Saudi Arabia for analysis. The isolates were screened for ESBL using VITEK(®) 2 Compact. Polymerase chain reaction (PCR) examination was used to determine TEM, SHV, and CTX-M genes. RESULTS: Our findings indicated that there is a high incidence of ESBLs among the E. coli isolated from UTI (23.1%). Our study also indicated that CTX-M genes are the most prevalent among the isolates at KFMMC followed by TEM class (6%), but there was also a higher percentage E. coli (3.4%) simultaneously harboring TEM and CTX-M genes. None of our isolates harbored the SHV genes. CONCLUSION: The findings document the threat of ESBL among E. coli isolates from UTI especially the CTX-M class in our hospital with the occurrence of these strains as etiologic agents of infection in the hospital and community.
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spelling pubmed-55562972017-08-25 Incidence and molecular characterization of the extended spectrum beta lactamase-producing Escherichia coli isolated from urinary tract infections in Eastern Saudi Arabia Mashwal, Fahad A. Safi, Salah H. El George, Siju K. Adam, Ahmed A. Jebakumar, Arulanantham Z. Saudi Med J Original Article OBJECTIVES: To find the prevalence of uropathogenic Escherichia coli (E. coli)-producing extended spectrum beta lactamase (ESBL) at King Fahd Military Medical Complex in Dhahran (KFMMC) and to detect the genes responsible for its production. In addition, we determined the pattern of multi-drug resistance among isolates. METHODS: A total of 117 uropathogenic E. coli isolates were collected from KFMMC over a period of 4 months from March 2014 to June 2014. These were received in the Microbiology Laboratory at Prince Sultan Military College of Health Sciences (PSMCHS), Dhahran, Saudi Arabia for analysis. The isolates were screened for ESBL using VITEK(®) 2 Compact. Polymerase chain reaction (PCR) examination was used to determine TEM, SHV, and CTX-M genes. RESULTS: Our findings indicated that there is a high incidence of ESBLs among the E. coli isolated from UTI (23.1%). Our study also indicated that CTX-M genes are the most prevalent among the isolates at KFMMC followed by TEM class (6%), but there was also a higher percentage E. coli (3.4%) simultaneously harboring TEM and CTX-M genes. None of our isolates harbored the SHV genes. CONCLUSION: The findings document the threat of ESBL among E. coli isolates from UTI especially the CTX-M class in our hospital with the occurrence of these strains as etiologic agents of infection in the hospital and community. Saudi Medical Journal 2017-08 /pmc/articles/PMC5556297/ /pubmed/28762433 http://dx.doi.org/10.15537/smj.2017.8.18578 Text en Copyright: © Saudi Medical Journal http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Mashwal, Fahad A.
Safi, Salah H. El
George, Siju K.
Adam, Ahmed A.
Jebakumar, Arulanantham Z.
Incidence and molecular characterization of the extended spectrum beta lactamase-producing Escherichia coli isolated from urinary tract infections in Eastern Saudi Arabia
title Incidence and molecular characterization of the extended spectrum beta lactamase-producing Escherichia coli isolated from urinary tract infections in Eastern Saudi Arabia
title_full Incidence and molecular characterization of the extended spectrum beta lactamase-producing Escherichia coli isolated from urinary tract infections in Eastern Saudi Arabia
title_fullStr Incidence and molecular characterization of the extended spectrum beta lactamase-producing Escherichia coli isolated from urinary tract infections in Eastern Saudi Arabia
title_full_unstemmed Incidence and molecular characterization of the extended spectrum beta lactamase-producing Escherichia coli isolated from urinary tract infections in Eastern Saudi Arabia
title_short Incidence and molecular characterization of the extended spectrum beta lactamase-producing Escherichia coli isolated from urinary tract infections in Eastern Saudi Arabia
title_sort incidence and molecular characterization of the extended spectrum beta lactamase-producing escherichia coli isolated from urinary tract infections in eastern saudi arabia
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5556297/
https://www.ncbi.nlm.nih.gov/pubmed/28762433
http://dx.doi.org/10.15537/smj.2017.8.18578
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