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The draft genome of the hyperthermophilic archaeon Pyrodictium delaneyi strain hulk, an iron and nitrate reducer, reveals the capacity for sulfate reduction

Pyrodictium delaneyi strain Hulk is a newly sequenced strain isolated from chimney samples collected from the Hulk sulfide mound on the main Endeavour Segment of the Juan de Fuca Ridge (47.9501 latitude, −129.0970 longitude, depth 2200 m) in the Northeast Pacific Ocean. The draft genome of strain Hu...

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Autores principales: Demey, Lucas M., Miller, Caitlin R., Manzella, Michael P, Spurbeck, Rachel R., Sandhu, Sukhinder K., Reguera, Gemma, Kashefi, Kazem
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5556600/
https://www.ncbi.nlm.nih.gov/pubmed/28814988
http://dx.doi.org/10.1186/s40793-017-0260-4
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author Demey, Lucas M.
Miller, Caitlin R.
Manzella, Michael P
Spurbeck, Rachel R.
Sandhu, Sukhinder K.
Reguera, Gemma
Kashefi, Kazem
author_facet Demey, Lucas M.
Miller, Caitlin R.
Manzella, Michael P
Spurbeck, Rachel R.
Sandhu, Sukhinder K.
Reguera, Gemma
Kashefi, Kazem
author_sort Demey, Lucas M.
collection PubMed
description Pyrodictium delaneyi strain Hulk is a newly sequenced strain isolated from chimney samples collected from the Hulk sulfide mound on the main Endeavour Segment of the Juan de Fuca Ridge (47.9501 latitude, −129.0970 longitude, depth 2200 m) in the Northeast Pacific Ocean. The draft genome of strain Hulk shared 99.77% similarity with the complete genome of the type strain Su06(T), which shares with strain Hulk the ability to reduce iron and nitrate for respiration. The annotation of the genome of strain Hulk identified genes for the reduction of several sulfur-containing electron acceptors, an unsuspected respiratory capability in this species that was experimentally confirmed for strain Hulk. This makes P. delaneyi strain Hulk the first hyperthermophilic archaeon known to gain energy for growth by reduction of iron, nitrate, and sulfur-containing electron acceptors. Here we present the most notable features of the genome of P. delaneyi strain Hulk and identify genes encoding proteins critical to its respiratory versatility at high temperatures. The description presented here corresponds to a draft genome sequence containing 2,042,801 bp in 9 contigs, 2019 protein-coding genes, 53 RNA genes, and 1365 hypothetical genes.
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spelling pubmed-55566002017-08-16 The draft genome of the hyperthermophilic archaeon Pyrodictium delaneyi strain hulk, an iron and nitrate reducer, reveals the capacity for sulfate reduction Demey, Lucas M. Miller, Caitlin R. Manzella, Michael P Spurbeck, Rachel R. Sandhu, Sukhinder K. Reguera, Gemma Kashefi, Kazem Stand Genomic Sci Extended Genome Report Pyrodictium delaneyi strain Hulk is a newly sequenced strain isolated from chimney samples collected from the Hulk sulfide mound on the main Endeavour Segment of the Juan de Fuca Ridge (47.9501 latitude, −129.0970 longitude, depth 2200 m) in the Northeast Pacific Ocean. The draft genome of strain Hulk shared 99.77% similarity with the complete genome of the type strain Su06(T), which shares with strain Hulk the ability to reduce iron and nitrate for respiration. The annotation of the genome of strain Hulk identified genes for the reduction of several sulfur-containing electron acceptors, an unsuspected respiratory capability in this species that was experimentally confirmed for strain Hulk. This makes P. delaneyi strain Hulk the first hyperthermophilic archaeon known to gain energy for growth by reduction of iron, nitrate, and sulfur-containing electron acceptors. Here we present the most notable features of the genome of P. delaneyi strain Hulk and identify genes encoding proteins critical to its respiratory versatility at high temperatures. The description presented here corresponds to a draft genome sequence containing 2,042,801 bp in 9 contigs, 2019 protein-coding genes, 53 RNA genes, and 1365 hypothetical genes. BioMed Central 2017-08-15 /pmc/articles/PMC5556600/ /pubmed/28814988 http://dx.doi.org/10.1186/s40793-017-0260-4 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Extended Genome Report
Demey, Lucas M.
Miller, Caitlin R.
Manzella, Michael P
Spurbeck, Rachel R.
Sandhu, Sukhinder K.
Reguera, Gemma
Kashefi, Kazem
The draft genome of the hyperthermophilic archaeon Pyrodictium delaneyi strain hulk, an iron and nitrate reducer, reveals the capacity for sulfate reduction
title The draft genome of the hyperthermophilic archaeon Pyrodictium delaneyi strain hulk, an iron and nitrate reducer, reveals the capacity for sulfate reduction
title_full The draft genome of the hyperthermophilic archaeon Pyrodictium delaneyi strain hulk, an iron and nitrate reducer, reveals the capacity for sulfate reduction
title_fullStr The draft genome of the hyperthermophilic archaeon Pyrodictium delaneyi strain hulk, an iron and nitrate reducer, reveals the capacity for sulfate reduction
title_full_unstemmed The draft genome of the hyperthermophilic archaeon Pyrodictium delaneyi strain hulk, an iron and nitrate reducer, reveals the capacity for sulfate reduction
title_short The draft genome of the hyperthermophilic archaeon Pyrodictium delaneyi strain hulk, an iron and nitrate reducer, reveals the capacity for sulfate reduction
title_sort draft genome of the hyperthermophilic archaeon pyrodictium delaneyi strain hulk, an iron and nitrate reducer, reveals the capacity for sulfate reduction
topic Extended Genome Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5556600/
https://www.ncbi.nlm.nih.gov/pubmed/28814988
http://dx.doi.org/10.1186/s40793-017-0260-4
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