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Overexpression of Arabidopsis P3B increases heat and low temperature stress tolerance in transgenic sweetpotato

BACKGROUND: Sweetpotato (Ipomoea batatas [L.] Lam) is suitable for growth on marginal lands due to its abiotic stress tolerance. However, severe environmental conditions including low temperature pose a serious threat to the productivity and expanded cultivation of this crop. In this study, we aimed...

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Autores principales: Ji, Chang Yoon, Jin, Rong, Xu, Zhen, Kim, Ho Soo, Lee, Chan-Ju, Kang, Le, Kim, So-Eun, Lee, Hyeong-Un, Lee, Joon Seol, Kang, Chang Ho, Chi, Yong Hun, Lee, Sang Yeol, Xie, Yiping, Li, Hongmin, Ma, Daifu, Kwak, Sang-Soo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5557506/
https://www.ncbi.nlm.nih.gov/pubmed/28806972
http://dx.doi.org/10.1186/s12870-017-1087-2
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author Ji, Chang Yoon
Jin, Rong
Xu, Zhen
Kim, Ho Soo
Lee, Chan-Ju
Kang, Le
Kim, So-Eun
Lee, Hyeong-Un
Lee, Joon Seol
Kang, Chang Ho
Chi, Yong Hun
Lee, Sang Yeol
Xie, Yiping
Li, Hongmin
Ma, Daifu
Kwak, Sang-Soo
author_facet Ji, Chang Yoon
Jin, Rong
Xu, Zhen
Kim, Ho Soo
Lee, Chan-Ju
Kang, Le
Kim, So-Eun
Lee, Hyeong-Un
Lee, Joon Seol
Kang, Chang Ho
Chi, Yong Hun
Lee, Sang Yeol
Xie, Yiping
Li, Hongmin
Ma, Daifu
Kwak, Sang-Soo
author_sort Ji, Chang Yoon
collection PubMed
description BACKGROUND: Sweetpotato (Ipomoea batatas [L.] Lam) is suitable for growth on marginal lands due to its abiotic stress tolerance. However, severe environmental conditions including low temperature pose a serious threat to the productivity and expanded cultivation of this crop. In this study, we aimed to develop sweetpotato plants with enhanced tolerance to temperature stress. RESULTS: P3 proteins are plant-specific ribosomal P-proteins that act as both protein and RNA chaperones to increase heat and cold stress tolerance in Arabidopsis. Here, we generated transgenic sweetpotato plants expressing the Arabidopsis ribosomal P3 (AtP3B) gene under the control of the CaMV 35S promoter (referred to as OP plants). Three OP lines (OP1, OP30, and OP32) were selected based on AtP3B transcript levels. The OP plants displayed greater heat tolerance and higher photosynthesis efficiency than wild type (WT) plants. The OP plants also exhibited enhanced low temperature tolerance, with higher photosynthesis efficiency and less membrane permeability than WT plants. In addition, OP plants had lower levels of hydrogen peroxide and higher activities of antioxidant enzymes such as peroxidase and catalase than WT plants under low temperature stress. The yields of tuberous roots and aerial parts of plants did not significantly differ between OP and WT plants under field cultivation. However, the tuberous roots of OP transgenic sweetpotato showed improved storage ability under low temperature conditions. CONCLUSIONS: The OP plants developed in this study exhibited increased tolerance to temperature stress and enhanced storage ability under low temperature compared to WT plants, suggesting that they could be used to enhance sustainable agriculture on marginal lands. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12870-017-1087-2) contains supplementary material, which is available to authorized users.
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spelling pubmed-55575062017-08-16 Overexpression of Arabidopsis P3B increases heat and low temperature stress tolerance in transgenic sweetpotato Ji, Chang Yoon Jin, Rong Xu, Zhen Kim, Ho Soo Lee, Chan-Ju Kang, Le Kim, So-Eun Lee, Hyeong-Un Lee, Joon Seol Kang, Chang Ho Chi, Yong Hun Lee, Sang Yeol Xie, Yiping Li, Hongmin Ma, Daifu Kwak, Sang-Soo BMC Plant Biol Research Article BACKGROUND: Sweetpotato (Ipomoea batatas [L.] Lam) is suitable for growth on marginal lands due to its abiotic stress tolerance. However, severe environmental conditions including low temperature pose a serious threat to the productivity and expanded cultivation of this crop. In this study, we aimed to develop sweetpotato plants with enhanced tolerance to temperature stress. RESULTS: P3 proteins are plant-specific ribosomal P-proteins that act as both protein and RNA chaperones to increase heat and cold stress tolerance in Arabidopsis. Here, we generated transgenic sweetpotato plants expressing the Arabidopsis ribosomal P3 (AtP3B) gene under the control of the CaMV 35S promoter (referred to as OP plants). Three OP lines (OP1, OP30, and OP32) were selected based on AtP3B transcript levels. The OP plants displayed greater heat tolerance and higher photosynthesis efficiency than wild type (WT) plants. The OP plants also exhibited enhanced low temperature tolerance, with higher photosynthesis efficiency and less membrane permeability than WT plants. In addition, OP plants had lower levels of hydrogen peroxide and higher activities of antioxidant enzymes such as peroxidase and catalase than WT plants under low temperature stress. The yields of tuberous roots and aerial parts of plants did not significantly differ between OP and WT plants under field cultivation. However, the tuberous roots of OP transgenic sweetpotato showed improved storage ability under low temperature conditions. CONCLUSIONS: The OP plants developed in this study exhibited increased tolerance to temperature stress and enhanced storage ability under low temperature compared to WT plants, suggesting that they could be used to enhance sustainable agriculture on marginal lands. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12870-017-1087-2) contains supplementary material, which is available to authorized users. BioMed Central 2017-08-14 /pmc/articles/PMC5557506/ /pubmed/28806972 http://dx.doi.org/10.1186/s12870-017-1087-2 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Ji, Chang Yoon
Jin, Rong
Xu, Zhen
Kim, Ho Soo
Lee, Chan-Ju
Kang, Le
Kim, So-Eun
Lee, Hyeong-Un
Lee, Joon Seol
Kang, Chang Ho
Chi, Yong Hun
Lee, Sang Yeol
Xie, Yiping
Li, Hongmin
Ma, Daifu
Kwak, Sang-Soo
Overexpression of Arabidopsis P3B increases heat and low temperature stress tolerance in transgenic sweetpotato
title Overexpression of Arabidopsis P3B increases heat and low temperature stress tolerance in transgenic sweetpotato
title_full Overexpression of Arabidopsis P3B increases heat and low temperature stress tolerance in transgenic sweetpotato
title_fullStr Overexpression of Arabidopsis P3B increases heat and low temperature stress tolerance in transgenic sweetpotato
title_full_unstemmed Overexpression of Arabidopsis P3B increases heat and low temperature stress tolerance in transgenic sweetpotato
title_short Overexpression of Arabidopsis P3B increases heat and low temperature stress tolerance in transgenic sweetpotato
title_sort overexpression of arabidopsis p3b increases heat and low temperature stress tolerance in transgenic sweetpotato
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5557506/
https://www.ncbi.nlm.nih.gov/pubmed/28806972
http://dx.doi.org/10.1186/s12870-017-1087-2
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