Cargando…
The frequency of class1 and 2 integrons in Pseudomonas aeruginosa strains isolated from burn patients in a burn center of Ahvaz, Iran
BACKGROUND: Pseudomonas aeruginosa is an opportunistic pathogen with the ability to cause severe nosocomial infections and remains a major problem in burn patients. This organism shows a remarkable antimicrobial resistance and is often resistant to multiple antibiotics. Integron genes as mobile gene...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5557579/ https://www.ncbi.nlm.nih.gov/pubmed/28813520 http://dx.doi.org/10.1371/journal.pone.0183061 |
_version_ | 1783257235525206016 |
---|---|
author | Khosravi, Azar Dokht Motahar, Moloudsadat Abbasi Montazeri, Effat |
author_facet | Khosravi, Azar Dokht Motahar, Moloudsadat Abbasi Montazeri, Effat |
author_sort | Khosravi, Azar Dokht |
collection | PubMed |
description | BACKGROUND: Pseudomonas aeruginosa is an opportunistic pathogen with the ability to cause severe nosocomial infections and remains a major problem in burn patients. This organism shows a remarkable antimicrobial resistance and is often resistant to multiple antibiotics. Integron genes as mobile genetic elements are playing an important role in the spread of P. aeruginosa antibiotic resistance. This study was aimed to investigate the occurrence of class 1, and 2 integron genes (int1, int2), among P. aeruginosa strains isolated from patients with burn infections. METHODS: In total 93 clinical isolates of P. aeruginosa were screened. The antimicrobial susceptibilities of 9 common antimicrobial agents were tested against the isolates using disk diffusion method. PCR amplification was performed on extracted DNAs for the detection of int1, and int2 genes using the set of specific primers. RESULTS: The majority of P. aeruginosa isolates were from wound infection (69.9%). In disk diffusion method, most isolates showed remarkable resistance to tested antibiotics with highest against gentamicin (94.62%) and ciprofloxacin (93.55%). PCR amplification revealed that 89(95.7%) of P. aeruginosa strains carried int1, but none of them harbored int2 genes. The distribution of int1 gene was highest in blood (100%), followed by wound isolates (95.38%). CONCLUSIONS: We demonstrated a high antimicrobial resistance among P. aeruginosa isolates in our setting. int1 was prevalent and seems to play an important role in multidrug resistance among the isolates. So, performance of antibiotic surveillance programs is necessary for choosing the appropriate therapy and management of infection control practices. |
format | Online Article Text |
id | pubmed-5557579 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-55575792017-08-25 The frequency of class1 and 2 integrons in Pseudomonas aeruginosa strains isolated from burn patients in a burn center of Ahvaz, Iran Khosravi, Azar Dokht Motahar, Moloudsadat Abbasi Montazeri, Effat PLoS One Research Article BACKGROUND: Pseudomonas aeruginosa is an opportunistic pathogen with the ability to cause severe nosocomial infections and remains a major problem in burn patients. This organism shows a remarkable antimicrobial resistance and is often resistant to multiple antibiotics. Integron genes as mobile genetic elements are playing an important role in the spread of P. aeruginosa antibiotic resistance. This study was aimed to investigate the occurrence of class 1, and 2 integron genes (int1, int2), among P. aeruginosa strains isolated from patients with burn infections. METHODS: In total 93 clinical isolates of P. aeruginosa were screened. The antimicrobial susceptibilities of 9 common antimicrobial agents were tested against the isolates using disk diffusion method. PCR amplification was performed on extracted DNAs for the detection of int1, and int2 genes using the set of specific primers. RESULTS: The majority of P. aeruginosa isolates were from wound infection (69.9%). In disk diffusion method, most isolates showed remarkable resistance to tested antibiotics with highest against gentamicin (94.62%) and ciprofloxacin (93.55%). PCR amplification revealed that 89(95.7%) of P. aeruginosa strains carried int1, but none of them harbored int2 genes. The distribution of int1 gene was highest in blood (100%), followed by wound isolates (95.38%). CONCLUSIONS: We demonstrated a high antimicrobial resistance among P. aeruginosa isolates in our setting. int1 was prevalent and seems to play an important role in multidrug resistance among the isolates. So, performance of antibiotic surveillance programs is necessary for choosing the appropriate therapy and management of infection control practices. Public Library of Science 2017-08-15 /pmc/articles/PMC5557579/ /pubmed/28813520 http://dx.doi.org/10.1371/journal.pone.0183061 Text en © 2017 Khosravi et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Khosravi, Azar Dokht Motahar, Moloudsadat Abbasi Montazeri, Effat The frequency of class1 and 2 integrons in Pseudomonas aeruginosa strains isolated from burn patients in a burn center of Ahvaz, Iran |
title | The frequency of class1 and 2 integrons in Pseudomonas aeruginosa strains isolated from burn patients in a burn center of Ahvaz, Iran |
title_full | The frequency of class1 and 2 integrons in Pseudomonas aeruginosa strains isolated from burn patients in a burn center of Ahvaz, Iran |
title_fullStr | The frequency of class1 and 2 integrons in Pseudomonas aeruginosa strains isolated from burn patients in a burn center of Ahvaz, Iran |
title_full_unstemmed | The frequency of class1 and 2 integrons in Pseudomonas aeruginosa strains isolated from burn patients in a burn center of Ahvaz, Iran |
title_short | The frequency of class1 and 2 integrons in Pseudomonas aeruginosa strains isolated from burn patients in a burn center of Ahvaz, Iran |
title_sort | frequency of class1 and 2 integrons in pseudomonas aeruginosa strains isolated from burn patients in a burn center of ahvaz, iran |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5557579/ https://www.ncbi.nlm.nih.gov/pubmed/28813520 http://dx.doi.org/10.1371/journal.pone.0183061 |
work_keys_str_mv | AT khosraviazardokht thefrequencyofclass1and2integronsinpseudomonasaeruginosastrainsisolatedfromburnpatientsinaburncenterofahvaziran AT motaharmoloudsadat thefrequencyofclass1and2integronsinpseudomonasaeruginosastrainsisolatedfromburnpatientsinaburncenterofahvaziran AT abbasimontazerieffat thefrequencyofclass1and2integronsinpseudomonasaeruginosastrainsisolatedfromburnpatientsinaburncenterofahvaziran AT khosraviazardokht frequencyofclass1and2integronsinpseudomonasaeruginosastrainsisolatedfromburnpatientsinaburncenterofahvaziran AT motaharmoloudsadat frequencyofclass1and2integronsinpseudomonasaeruginosastrainsisolatedfromburnpatientsinaburncenterofahvaziran AT abbasimontazerieffat frequencyofclass1and2integronsinpseudomonasaeruginosastrainsisolatedfromburnpatientsinaburncenterofahvaziran |