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Synthesis-free PET imaging of brown adipose tissue and TSPO via combination of disulfiram and (64)CuCl(2)

PET imaging is a widely applicable but a very expensive technology. On-site synthesis is one important contributor to the high cost. In this report, we demonstrated the feasibility of a synthesis-free method for PET imaging of brown adipose tissue (BAT) and translocator protein 18 kDa (TSPO) via a c...

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Autores principales: Yang, Jing, Yang, Jian, Wang, Lu, Moore, Anna, Liang, Steven H., Ran, Chongzhao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5557754/
https://www.ncbi.nlm.nih.gov/pubmed/28811616
http://dx.doi.org/10.1038/s41598-017-09018-2
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author Yang, Jing
Yang, Jian
Wang, Lu
Moore, Anna
Liang, Steven H.
Ran, Chongzhao
author_facet Yang, Jing
Yang, Jian
Wang, Lu
Moore, Anna
Liang, Steven H.
Ran, Chongzhao
author_sort Yang, Jing
collection PubMed
description PET imaging is a widely applicable but a very expensive technology. On-site synthesis is one important contributor to the high cost. In this report, we demonstrated the feasibility of a synthesis-free method for PET imaging of brown adipose tissue (BAT) and translocator protein 18 kDa (TSPO) via a combination of disulfiram, an FDA approved drug for alcoholism, and (64)CuCl(2) (termed (64)Cu-Dis). In this method, a step-wise injection protocol of (64)CuCl(2) and disulfiram was used to accomplish the purpose of synthesis-free. Specifically, disulfiram, an inactive (64)Cu ligand, was first injected to allow it to metabolize into diethyldithiocarbamate (DDC), a strong (64)Cu ligand, which can chelate (64)CuCl(2) from the following injection to form the actual PET tracer in situ. Our blocking studies, western blot, and tissue histological imaging suggested that the observed BAT contrast was due to (64)Cu-Dis binding to TSPO, which was further confirmed as a specific biomarker for BAT imaging using [(18)F]-F-DPA, a TSPO-specific PET tracer. Our studies, for the first time, demonstrated that TSPO could serve as a potential imaging biomarker for BAT. We believe that our strategy could be extended to other targets while significantly reducing the cost of PET imaging.
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spelling pubmed-55577542017-08-16 Synthesis-free PET imaging of brown adipose tissue and TSPO via combination of disulfiram and (64)CuCl(2) Yang, Jing Yang, Jian Wang, Lu Moore, Anna Liang, Steven H. Ran, Chongzhao Sci Rep Article PET imaging is a widely applicable but a very expensive technology. On-site synthesis is one important contributor to the high cost. In this report, we demonstrated the feasibility of a synthesis-free method for PET imaging of brown adipose tissue (BAT) and translocator protein 18 kDa (TSPO) via a combination of disulfiram, an FDA approved drug for alcoholism, and (64)CuCl(2) (termed (64)Cu-Dis). In this method, a step-wise injection protocol of (64)CuCl(2) and disulfiram was used to accomplish the purpose of synthesis-free. Specifically, disulfiram, an inactive (64)Cu ligand, was first injected to allow it to metabolize into diethyldithiocarbamate (DDC), a strong (64)Cu ligand, which can chelate (64)CuCl(2) from the following injection to form the actual PET tracer in situ. Our blocking studies, western blot, and tissue histological imaging suggested that the observed BAT contrast was due to (64)Cu-Dis binding to TSPO, which was further confirmed as a specific biomarker for BAT imaging using [(18)F]-F-DPA, a TSPO-specific PET tracer. Our studies, for the first time, demonstrated that TSPO could serve as a potential imaging biomarker for BAT. We believe that our strategy could be extended to other targets while significantly reducing the cost of PET imaging. Nature Publishing Group UK 2017-08-15 /pmc/articles/PMC5557754/ /pubmed/28811616 http://dx.doi.org/10.1038/s41598-017-09018-2 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Yang, Jing
Yang, Jian
Wang, Lu
Moore, Anna
Liang, Steven H.
Ran, Chongzhao
Synthesis-free PET imaging of brown adipose tissue and TSPO via combination of disulfiram and (64)CuCl(2)
title Synthesis-free PET imaging of brown adipose tissue and TSPO via combination of disulfiram and (64)CuCl(2)
title_full Synthesis-free PET imaging of brown adipose tissue and TSPO via combination of disulfiram and (64)CuCl(2)
title_fullStr Synthesis-free PET imaging of brown adipose tissue and TSPO via combination of disulfiram and (64)CuCl(2)
title_full_unstemmed Synthesis-free PET imaging of brown adipose tissue and TSPO via combination of disulfiram and (64)CuCl(2)
title_short Synthesis-free PET imaging of brown adipose tissue and TSPO via combination of disulfiram and (64)CuCl(2)
title_sort synthesis-free pet imaging of brown adipose tissue and tspo via combination of disulfiram and (64)cucl(2)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5557754/
https://www.ncbi.nlm.nih.gov/pubmed/28811616
http://dx.doi.org/10.1038/s41598-017-09018-2
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