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The antibacterial and antifungal activity of six essential oils and their cyto/genotoxicity to human HEL 12469 cells

Six essential oils (from oregano, thyme, clove, lavender, clary sage, and arborvitae) exhibited different antibacterial and antifungal properties. Antimicrobial activity was shown against pathogenic (Escherichia coli, Salmonella typhimurium, Yersinia enterocolitica, Staphylococcus aureus, Listeria m...

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Autores principales: Puškárová, Andrea, Bučková, Mária, Kraková, Lucia, Pangallo, Domenico, Kozics, Katarína
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5557807/
https://www.ncbi.nlm.nih.gov/pubmed/28811611
http://dx.doi.org/10.1038/s41598-017-08673-9
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author Puškárová, Andrea
Bučková, Mária
Kraková, Lucia
Pangallo, Domenico
Kozics, Katarína
author_facet Puškárová, Andrea
Bučková, Mária
Kraková, Lucia
Pangallo, Domenico
Kozics, Katarína
author_sort Puškárová, Andrea
collection PubMed
description Six essential oils (from oregano, thyme, clove, lavender, clary sage, and arborvitae) exhibited different antibacterial and antifungal properties. Antimicrobial activity was shown against pathogenic (Escherichia coli, Salmonella typhimurium, Yersinia enterocolitica, Staphylococcus aureus, Listeria monocytogenes, and Enterococcus faecalis) and environmental bacteria (Bacillus cereus, Arthrobacter protophormiae, Pseudomonas fragi) and fungi (Chaetomium globosum, Penicillium chrysogenum, Cladosporium cladosporoides, Alternaria alternata, and Aspergillus fumigatus). Oregano, thyme, clove and arborvitae showed very strong antibacterial activity against all tested strains at both full strength and reduced concentrations. These essential oils showed different fungistatic and fungicidal activities when tested by direct application and in the vapor phase. The genotoxic effects of these oils on HEL 12469 human embryo lung cells were evaluated using an alkaline comet assay for the first time, revealing that none of the oils induced significant DNA damage in vitro after 24 h. This study provides novel approaches for assessing the antimicrobial potential of essential oils in both direct contact and the vapor phase and also demonstrates the valuable properties of the phenol-free arborvitae oil. These results suggest that all the tested essential oils might be used as broad-spectrum anti-microbial agents for decontaminating an indoor environment.
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spelling pubmed-55578072017-08-16 The antibacterial and antifungal activity of six essential oils and their cyto/genotoxicity to human HEL 12469 cells Puškárová, Andrea Bučková, Mária Kraková, Lucia Pangallo, Domenico Kozics, Katarína Sci Rep Article Six essential oils (from oregano, thyme, clove, lavender, clary sage, and arborvitae) exhibited different antibacterial and antifungal properties. Antimicrobial activity was shown against pathogenic (Escherichia coli, Salmonella typhimurium, Yersinia enterocolitica, Staphylococcus aureus, Listeria monocytogenes, and Enterococcus faecalis) and environmental bacteria (Bacillus cereus, Arthrobacter protophormiae, Pseudomonas fragi) and fungi (Chaetomium globosum, Penicillium chrysogenum, Cladosporium cladosporoides, Alternaria alternata, and Aspergillus fumigatus). Oregano, thyme, clove and arborvitae showed very strong antibacterial activity against all tested strains at both full strength and reduced concentrations. These essential oils showed different fungistatic and fungicidal activities when tested by direct application and in the vapor phase. The genotoxic effects of these oils on HEL 12469 human embryo lung cells were evaluated using an alkaline comet assay for the first time, revealing that none of the oils induced significant DNA damage in vitro after 24 h. This study provides novel approaches for assessing the antimicrobial potential of essential oils in both direct contact and the vapor phase and also demonstrates the valuable properties of the phenol-free arborvitae oil. These results suggest that all the tested essential oils might be used as broad-spectrum anti-microbial agents for decontaminating an indoor environment. Nature Publishing Group UK 2017-08-15 /pmc/articles/PMC5557807/ /pubmed/28811611 http://dx.doi.org/10.1038/s41598-017-08673-9 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Puškárová, Andrea
Bučková, Mária
Kraková, Lucia
Pangallo, Domenico
Kozics, Katarína
The antibacterial and antifungal activity of six essential oils and their cyto/genotoxicity to human HEL 12469 cells
title The antibacterial and antifungal activity of six essential oils and their cyto/genotoxicity to human HEL 12469 cells
title_full The antibacterial and antifungal activity of six essential oils and their cyto/genotoxicity to human HEL 12469 cells
title_fullStr The antibacterial and antifungal activity of six essential oils and their cyto/genotoxicity to human HEL 12469 cells
title_full_unstemmed The antibacterial and antifungal activity of six essential oils and their cyto/genotoxicity to human HEL 12469 cells
title_short The antibacterial and antifungal activity of six essential oils and their cyto/genotoxicity to human HEL 12469 cells
title_sort antibacterial and antifungal activity of six essential oils and their cyto/genotoxicity to human hel 12469 cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5557807/
https://www.ncbi.nlm.nih.gov/pubmed/28811611
http://dx.doi.org/10.1038/s41598-017-08673-9
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