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IgE Trimers Drive SPE-7 Cytokinergic Activity

Degranulation of mast cells and basophils, with release of agents of the allergic response, ensues when multivalent antigens bind to and cross-link the cells’ receptor-bound IgE antibodies. A widely used commercial monoclonal IgE antibody, SPE-7 IgE from Sigma, was found to possess the radically ano...

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Autores principales: Bax, Heather J., Bowen, Holly, Beavil, Rebecca L., Chung, Raymond, Ward, Malcolm, Davies, Anna M., Dodev, Tihomir S., McDonnell, James M., Beavil, Andrew J., Sutton, Brian J., Gould, Hannah J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5557881/
https://www.ncbi.nlm.nih.gov/pubmed/28811536
http://dx.doi.org/10.1038/s41598-017-08212-6
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author Bax, Heather J.
Bowen, Holly
Beavil, Rebecca L.
Chung, Raymond
Ward, Malcolm
Davies, Anna M.
Dodev, Tihomir S.
McDonnell, James M.
Beavil, Andrew J.
Sutton, Brian J.
Gould, Hannah J.
author_facet Bax, Heather J.
Bowen, Holly
Beavil, Rebecca L.
Chung, Raymond
Ward, Malcolm
Davies, Anna M.
Dodev, Tihomir S.
McDonnell, James M.
Beavil, Andrew J.
Sutton, Brian J.
Gould, Hannah J.
author_sort Bax, Heather J.
collection PubMed
description Degranulation of mast cells and basophils, with release of agents of the allergic response, ensues when multivalent antigens bind to and cross-link the cells’ receptor-bound IgE antibodies. A widely used commercial monoclonal IgE antibody, SPE-7 IgE from Sigma, was found to possess the radically anomalous property, termed “cytokinergic”, of inducing basophil degranulation without the intervention of an antigen. We show here that the IgE monomer, freed of protein contaminants, is devoid of this activity, and that the source of the anomaly is a trace impurity, identified as a dissociation-resistant IgE trimer. Possible models for the formation of IgE trimers and the manner in which they cross-link cell surface receptors are suggested herein.
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spelling pubmed-55578812017-08-16 IgE Trimers Drive SPE-7 Cytokinergic Activity Bax, Heather J. Bowen, Holly Beavil, Rebecca L. Chung, Raymond Ward, Malcolm Davies, Anna M. Dodev, Tihomir S. McDonnell, James M. Beavil, Andrew J. Sutton, Brian J. Gould, Hannah J. Sci Rep Article Degranulation of mast cells and basophils, with release of agents of the allergic response, ensues when multivalent antigens bind to and cross-link the cells’ receptor-bound IgE antibodies. A widely used commercial monoclonal IgE antibody, SPE-7 IgE from Sigma, was found to possess the radically anomalous property, termed “cytokinergic”, of inducing basophil degranulation without the intervention of an antigen. We show here that the IgE monomer, freed of protein contaminants, is devoid of this activity, and that the source of the anomaly is a trace impurity, identified as a dissociation-resistant IgE trimer. Possible models for the formation of IgE trimers and the manner in which they cross-link cell surface receptors are suggested herein. Nature Publishing Group UK 2017-08-15 /pmc/articles/PMC5557881/ /pubmed/28811536 http://dx.doi.org/10.1038/s41598-017-08212-6 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Bax, Heather J.
Bowen, Holly
Beavil, Rebecca L.
Chung, Raymond
Ward, Malcolm
Davies, Anna M.
Dodev, Tihomir S.
McDonnell, James M.
Beavil, Andrew J.
Sutton, Brian J.
Gould, Hannah J.
IgE Trimers Drive SPE-7 Cytokinergic Activity
title IgE Trimers Drive SPE-7 Cytokinergic Activity
title_full IgE Trimers Drive SPE-7 Cytokinergic Activity
title_fullStr IgE Trimers Drive SPE-7 Cytokinergic Activity
title_full_unstemmed IgE Trimers Drive SPE-7 Cytokinergic Activity
title_short IgE Trimers Drive SPE-7 Cytokinergic Activity
title_sort ige trimers drive spe-7 cytokinergic activity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5557881/
https://www.ncbi.nlm.nih.gov/pubmed/28811536
http://dx.doi.org/10.1038/s41598-017-08212-6
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