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A new method to investigate the catalytic mechanism of YhdE pyrophosphatase by using a pyrophosphate fluorescence probe

YhdE is a Maf (multicopy associated filamentation) proteins from Escherichia coli which exhibits pyrophosphatase activity towards selected nucleotides, although its catalytic mechanism remains unclear. Herein we used a novel fluorescence probe (4-isoACBA–Zn(II) complex) to characterize the enzymatic...

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Detalles Bibliográficos
Autores principales:	Shen, Qingya, Tan, Hongwei, Xing, Guo-wen, Zheng, Jimin, Jia, Zongchao
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2017
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5557916/ https://www.ncbi.nlm.nih.gov/pubmed/28811554 http://dx.doi.org/10.1038/s41598-017-08368-1

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