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Bacterial diversity among four healthcare-associated institutes in Taiwan

Indoor microbial communities have important implications for human health, especially in health-care institutes (HCIs). The factors that determine the diversity and composition of microbiomes in a built environment remain unclear. Herein, we used 16S rRNA amplicon sequencing to investigate the relat...

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Autores principales: Chen, Chang-Hua, Lin, Yaw-Ling, Chen, Kuan-Hsueh, Chen, Wen-Pei, Chen, Zhao-Feng, Kuo, Han-Yueh, Hung, Hsueh-Fen, Tang, Chuan Yi, Liou, Ming-Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5557925/
https://www.ncbi.nlm.nih.gov/pubmed/28811583
http://dx.doi.org/10.1038/s41598-017-08679-3
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author Chen, Chang-Hua
Lin, Yaw-Ling
Chen, Kuan-Hsueh
Chen, Wen-Pei
Chen, Zhao-Feng
Kuo, Han-Yueh
Hung, Hsueh-Fen
Tang, Chuan Yi
Liou, Ming-Li
author_facet Chen, Chang-Hua
Lin, Yaw-Ling
Chen, Kuan-Hsueh
Chen, Wen-Pei
Chen, Zhao-Feng
Kuo, Han-Yueh
Hung, Hsueh-Fen
Tang, Chuan Yi
Liou, Ming-Li
author_sort Chen, Chang-Hua
collection PubMed
description Indoor microbial communities have important implications for human health, especially in health-care institutes (HCIs). The factors that determine the diversity and composition of microbiomes in a built environment remain unclear. Herein, we used 16S rRNA amplicon sequencing to investigate the relationships between building attributes and surface bacterial communities among four HCIs located in three buildings. We examined the surface bacterial communities and environmental parameters in the buildings supplied with different ventilation types and compared the results using a Dirichlet multinomial mixture (DMM)-based approach. A total of 203 samples from the four HCIs were analyzed. Four bacterial communities were grouped using the DMM-based approach, which were highly similar to those in the 4 HCIs. The α-diversity and β-diversity in the naturally ventilated building were different from the conditioner-ventilated building. The bacterial source composition varied across each building. Nine genera were found as the core microbiota shared by all the areas, of which Acinetobacter, Enterobacter, Pseudomonas, and Staphylococcus are regarded as healthcare-associated pathogens (HAPs). The observed relationship between environmental parameters such as core microbiota and surface bacterial diversity suggests that we might manage indoor environments by creating new sanitation protocols, adjusting the ventilation design, and further understanding the transmission routes of HAPs.
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spelling pubmed-55579252017-08-16 Bacterial diversity among four healthcare-associated institutes in Taiwan Chen, Chang-Hua Lin, Yaw-Ling Chen, Kuan-Hsueh Chen, Wen-Pei Chen, Zhao-Feng Kuo, Han-Yueh Hung, Hsueh-Fen Tang, Chuan Yi Liou, Ming-Li Sci Rep Article Indoor microbial communities have important implications for human health, especially in health-care institutes (HCIs). The factors that determine the diversity and composition of microbiomes in a built environment remain unclear. Herein, we used 16S rRNA amplicon sequencing to investigate the relationships between building attributes and surface bacterial communities among four HCIs located in three buildings. We examined the surface bacterial communities and environmental parameters in the buildings supplied with different ventilation types and compared the results using a Dirichlet multinomial mixture (DMM)-based approach. A total of 203 samples from the four HCIs were analyzed. Four bacterial communities were grouped using the DMM-based approach, which were highly similar to those in the 4 HCIs. The α-diversity and β-diversity in the naturally ventilated building were different from the conditioner-ventilated building. The bacterial source composition varied across each building. Nine genera were found as the core microbiota shared by all the areas, of which Acinetobacter, Enterobacter, Pseudomonas, and Staphylococcus are regarded as healthcare-associated pathogens (HAPs). The observed relationship between environmental parameters such as core microbiota and surface bacterial diversity suggests that we might manage indoor environments by creating new sanitation protocols, adjusting the ventilation design, and further understanding the transmission routes of HAPs. Nature Publishing Group UK 2017-08-15 /pmc/articles/PMC5557925/ /pubmed/28811583 http://dx.doi.org/10.1038/s41598-017-08679-3 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Chen, Chang-Hua
Lin, Yaw-Ling
Chen, Kuan-Hsueh
Chen, Wen-Pei
Chen, Zhao-Feng
Kuo, Han-Yueh
Hung, Hsueh-Fen
Tang, Chuan Yi
Liou, Ming-Li
Bacterial diversity among four healthcare-associated institutes in Taiwan
title Bacterial diversity among four healthcare-associated institutes in Taiwan
title_full Bacterial diversity among four healthcare-associated institutes in Taiwan
title_fullStr Bacterial diversity among four healthcare-associated institutes in Taiwan
title_full_unstemmed Bacterial diversity among four healthcare-associated institutes in Taiwan
title_short Bacterial diversity among four healthcare-associated institutes in Taiwan
title_sort bacterial diversity among four healthcare-associated institutes in taiwan
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5557925/
https://www.ncbi.nlm.nih.gov/pubmed/28811583
http://dx.doi.org/10.1038/s41598-017-08679-3
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