Cargando…
Yangzheng Sanjie decoction regulates proliferation and apoptosis of gastric cancer cells by enhancing let-7a expression
AIM: To explore the let-7a-mediated anti-cancer effect of Yangzheng Sanjie decoction (YZSJD) in gastric cancer (GC) cells. METHODS: YZSJD-containing serum (YCS) was prepared using traditional Chinese medicine serum pharmacology methods. After YCS treatment, cell proliferation and apoptosis were asse...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Baishideng Publishing Group Inc
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5558117/ https://www.ncbi.nlm.nih.gov/pubmed/28852313 http://dx.doi.org/10.3748/wjg.v23.i30.5538 |
_version_ | 1783257340682698752 |
---|---|
author | Deng, Hai-Xia Yu, Ya-Ya Zhou, Ao-Qiang Zhu, Jing-Li Luo, Li-Na Chen, Wan-Qun Hu, Ling Chen, Geng-Xin |
author_facet | Deng, Hai-Xia Yu, Ya-Ya Zhou, Ao-Qiang Zhu, Jing-Li Luo, Li-Na Chen, Wan-Qun Hu, Ling Chen, Geng-Xin |
author_sort | Deng, Hai-Xia |
collection | PubMed |
description | AIM: To explore the let-7a-mediated anti-cancer effect of Yangzheng Sanjie decoction (YZSJD) in gastric cancer (GC) cells. METHODS: YZSJD-containing serum (YCS) was prepared using traditional Chinese medicine serum pharmacology methods. After YCS treatment, cell proliferation and apoptosis were assessed by cell counting kit-8 assay and flow cytometry, respectively, and miRNA expression profiles were determined using qPCR arrays. Let-7a expression was examined by in situ hybridization in GC tissues and by qPCR in GC cells. c-Myc protein expression was detected by immunohistochemistry in GC tissues, and by Western blot in cell lines. RESULTS: YZSJD significantly inhibited proliferation and induced apoptosis in AGS and HS-746T GC cells. After treatment with YCS, the miRNA expression profiles were altered and the reduced let-7a levels in both cell lines were up-regulated, accompanied by a decrease in c-Myc expression. Moreover, decreased let-7a expression and increased c-Myc expression were observed during the progression of gastric mucosa cancerization. CONCLUSION: YZSJD inhibits proliferation and induces apoptosis of GC cells by restoring the aberrant expression of let-7a and c-Myc. |
format | Online Article Text |
id | pubmed-5558117 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Baishideng Publishing Group Inc |
record_format | MEDLINE/PubMed |
spelling | pubmed-55581172017-08-29 Yangzheng Sanjie decoction regulates proliferation and apoptosis of gastric cancer cells by enhancing let-7a expression Deng, Hai-Xia Yu, Ya-Ya Zhou, Ao-Qiang Zhu, Jing-Li Luo, Li-Na Chen, Wan-Qun Hu, Ling Chen, Geng-Xin World J Gastroenterol Basic Study AIM: To explore the let-7a-mediated anti-cancer effect of Yangzheng Sanjie decoction (YZSJD) in gastric cancer (GC) cells. METHODS: YZSJD-containing serum (YCS) was prepared using traditional Chinese medicine serum pharmacology methods. After YCS treatment, cell proliferation and apoptosis were assessed by cell counting kit-8 assay and flow cytometry, respectively, and miRNA expression profiles were determined using qPCR arrays. Let-7a expression was examined by in situ hybridization in GC tissues and by qPCR in GC cells. c-Myc protein expression was detected by immunohistochemistry in GC tissues, and by Western blot in cell lines. RESULTS: YZSJD significantly inhibited proliferation and induced apoptosis in AGS and HS-746T GC cells. After treatment with YCS, the miRNA expression profiles were altered and the reduced let-7a levels in both cell lines were up-regulated, accompanied by a decrease in c-Myc expression. Moreover, decreased let-7a expression and increased c-Myc expression were observed during the progression of gastric mucosa cancerization. CONCLUSION: YZSJD inhibits proliferation and induces apoptosis of GC cells by restoring the aberrant expression of let-7a and c-Myc. Baishideng Publishing Group Inc 2017-08-14 2017-08-14 /pmc/articles/PMC5558117/ /pubmed/28852313 http://dx.doi.org/10.3748/wjg.v23.i30.5538 Text en ©The Author(s) 2017. Published by Baishideng Publishing Group Inc. All rights reserved. http://creativecommons.org/licenses/by-nc/4.0/ This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. |
spellingShingle | Basic Study Deng, Hai-Xia Yu, Ya-Ya Zhou, Ao-Qiang Zhu, Jing-Li Luo, Li-Na Chen, Wan-Qun Hu, Ling Chen, Geng-Xin Yangzheng Sanjie decoction regulates proliferation and apoptosis of gastric cancer cells by enhancing let-7a expression |
title | Yangzheng Sanjie decoction regulates proliferation and apoptosis of gastric cancer cells by enhancing let-7a expression |
title_full | Yangzheng Sanjie decoction regulates proliferation and apoptosis of gastric cancer cells by enhancing let-7a expression |
title_fullStr | Yangzheng Sanjie decoction regulates proliferation and apoptosis of gastric cancer cells by enhancing let-7a expression |
title_full_unstemmed | Yangzheng Sanjie decoction regulates proliferation and apoptosis of gastric cancer cells by enhancing let-7a expression |
title_short | Yangzheng Sanjie decoction regulates proliferation and apoptosis of gastric cancer cells by enhancing let-7a expression |
title_sort | yangzheng sanjie decoction regulates proliferation and apoptosis of gastric cancer cells by enhancing let-7a expression |
topic | Basic Study |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5558117/ https://www.ncbi.nlm.nih.gov/pubmed/28852313 http://dx.doi.org/10.3748/wjg.v23.i30.5538 |
work_keys_str_mv | AT denghaixia yangzhengsanjiedecoctionregulatesproliferationandapoptosisofgastriccancercellsbyenhancinglet7aexpression AT yuyaya yangzhengsanjiedecoctionregulatesproliferationandapoptosisofgastriccancercellsbyenhancinglet7aexpression AT zhouaoqiang yangzhengsanjiedecoctionregulatesproliferationandapoptosisofgastriccancercellsbyenhancinglet7aexpression AT zhujingli yangzhengsanjiedecoctionregulatesproliferationandapoptosisofgastriccancercellsbyenhancinglet7aexpression AT luolina yangzhengsanjiedecoctionregulatesproliferationandapoptosisofgastriccancercellsbyenhancinglet7aexpression AT chenwanqun yangzhengsanjiedecoctionregulatesproliferationandapoptosisofgastriccancercellsbyenhancinglet7aexpression AT huling yangzhengsanjiedecoctionregulatesproliferationandapoptosisofgastriccancercellsbyenhancinglet7aexpression AT chengengxin yangzhengsanjiedecoctionregulatesproliferationandapoptosisofgastriccancercellsbyenhancinglet7aexpression |