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Protective effects of Dendrobium nobile Lindl. alkaloids on amyloid beta (25–35)-induced neuronal injury

Dendrobium nobile Lindl. alkaloids (DNLA), the active ingredients of a traditional Chinese medicine Dendrobium, have been shown to have anti-oxidative effects, anti-inflammatory action, and protective effect on neurons against oxygen-glucose deprivation. However, it is not clear whether DNLA reduces...

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Autores principales: Zhang, Wei, Wu, Qin, Lu, Yan-liu, Gong, Qi-hai, Zhang, Feng, Shi, Jing-shan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5558493/
https://www.ncbi.nlm.nih.gov/pubmed/28852396
http://dx.doi.org/10.4103/1673-5374.211193
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author Zhang, Wei
Wu, Qin
Lu, Yan-liu
Gong, Qi-hai
Zhang, Feng
Shi, Jing-shan
author_facet Zhang, Wei
Wu, Qin
Lu, Yan-liu
Gong, Qi-hai
Zhang, Feng
Shi, Jing-shan
author_sort Zhang, Wei
collection PubMed
description Dendrobium nobile Lindl. alkaloids (DNLA), the active ingredients of a traditional Chinese medicine Dendrobium, have been shown to have anti-oxidative effects, anti-inflammatory action, and protective effect on neurons against oxygen-glucose deprivation. However, it is not clear whether DNLA reduces amyloid-beta (Aβ)-induced neuronal injury. In this study, cortical neurons were treated with DNLA at different concentrations (0.025, 0.25, and 2.5 mg/L) for 24 hours, followed by administration of Aβ(25–35) (10 μM). Aβ(25–35) treatments increased cell injury as determined by the leakage of lactate dehydrogenase, which was accompanied by chromatin condensation and mitochondrial tumefaction. The damage caused by Aβ(25–35) on these cellular properties was markedly attenuated when cells were pretreated with DNLA. Treatment with Aβ(25–35) down-regulated the expressions of postsynaptic density-95 mRNA and decreased the protein expression of synaptophysin and postsynaptic density-95, all changes were significantly reduced by pretreatment of cells with DNLA. These findings suggest that DNLA reduces the cytotoxicity induced by Aβ(25–35) in rat primary cultured neurons. The protective mechanism that DNLA confers on the synaptic integrity of cultured neurons might be mediated, at least in part, through the upregulation of neurogenesis related proteins synaptophysin and postsynaptic density-95.
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spelling pubmed-55584932017-08-29 Protective effects of Dendrobium nobile Lindl. alkaloids on amyloid beta (25–35)-induced neuronal injury Zhang, Wei Wu, Qin Lu, Yan-liu Gong, Qi-hai Zhang, Feng Shi, Jing-shan Neural Regen Res Research Article Dendrobium nobile Lindl. alkaloids (DNLA), the active ingredients of a traditional Chinese medicine Dendrobium, have been shown to have anti-oxidative effects, anti-inflammatory action, and protective effect on neurons against oxygen-glucose deprivation. However, it is not clear whether DNLA reduces amyloid-beta (Aβ)-induced neuronal injury. In this study, cortical neurons were treated with DNLA at different concentrations (0.025, 0.25, and 2.5 mg/L) for 24 hours, followed by administration of Aβ(25–35) (10 μM). Aβ(25–35) treatments increased cell injury as determined by the leakage of lactate dehydrogenase, which was accompanied by chromatin condensation and mitochondrial tumefaction. The damage caused by Aβ(25–35) on these cellular properties was markedly attenuated when cells were pretreated with DNLA. Treatment with Aβ(25–35) down-regulated the expressions of postsynaptic density-95 mRNA and decreased the protein expression of synaptophysin and postsynaptic density-95, all changes were significantly reduced by pretreatment of cells with DNLA. These findings suggest that DNLA reduces the cytotoxicity induced by Aβ(25–35) in rat primary cultured neurons. The protective mechanism that DNLA confers on the synaptic integrity of cultured neurons might be mediated, at least in part, through the upregulation of neurogenesis related proteins synaptophysin and postsynaptic density-95. Medknow Publications & Media Pvt Ltd 2017-07 /pmc/articles/PMC5558493/ /pubmed/28852396 http://dx.doi.org/10.4103/1673-5374.211193 Text en Copyright: © Neural Regeneration Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Research Article
Zhang, Wei
Wu, Qin
Lu, Yan-liu
Gong, Qi-hai
Zhang, Feng
Shi, Jing-shan
Protective effects of Dendrobium nobile Lindl. alkaloids on amyloid beta (25–35)-induced neuronal injury
title Protective effects of Dendrobium nobile Lindl. alkaloids on amyloid beta (25–35)-induced neuronal injury
title_full Protective effects of Dendrobium nobile Lindl. alkaloids on amyloid beta (25–35)-induced neuronal injury
title_fullStr Protective effects of Dendrobium nobile Lindl. alkaloids on amyloid beta (25–35)-induced neuronal injury
title_full_unstemmed Protective effects of Dendrobium nobile Lindl. alkaloids on amyloid beta (25–35)-induced neuronal injury
title_short Protective effects of Dendrobium nobile Lindl. alkaloids on amyloid beta (25–35)-induced neuronal injury
title_sort protective effects of dendrobium nobile lindl. alkaloids on amyloid beta (25–35)-induced neuronal injury
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5558493/
https://www.ncbi.nlm.nih.gov/pubmed/28852396
http://dx.doi.org/10.4103/1673-5374.211193
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