Cargando…

Twin birth changes DNA methylation of subsequent siblings

We asked if twin birth influences the DNA methylation of subsequent siblings. We measured whole blood methylation using the HumanMethylation450 array for siblings from two twin and family studies in Australia and Korea. We compared the means and correlations in methylation between pairs of siblings...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Shuai, Kim, Eunae, Wong, Ee Ming, Joo, Ji-Hoon Eric, Nguyen, Tuong L., Stone, Jennifer, Song, Yun-Mi, Flander, Louisa B., Saffery, Richard, Giles, Graham G., Southey, Melissa C., Sung, Joohon, Hopper, John L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5559542/
https://www.ncbi.nlm.nih.gov/pubmed/28814741
http://dx.doi.org/10.1038/s41598-017-08595-6
_version_ 1783257538782822400
author Li, Shuai
Kim, Eunae
Wong, Ee Ming
Joo, Ji-Hoon Eric
Nguyen, Tuong L.
Stone, Jennifer
Song, Yun-Mi
Flander, Louisa B.
Saffery, Richard
Giles, Graham G.
Southey, Melissa C.
Sung, Joohon
Hopper, John L.
author_facet Li, Shuai
Kim, Eunae
Wong, Ee Ming
Joo, Ji-Hoon Eric
Nguyen, Tuong L.
Stone, Jennifer
Song, Yun-Mi
Flander, Louisa B.
Saffery, Richard
Giles, Graham G.
Southey, Melissa C.
Sung, Joohon
Hopper, John L.
author_sort Li, Shuai
collection PubMed
description We asked if twin birth influences the DNA methylation of subsequent siblings. We measured whole blood methylation using the HumanMethylation450 array for siblings from two twin and family studies in Australia and Korea. We compared the means and correlations in methylation between pairs of siblings born before a twin birth (BT siblings), born on either side of a twin birth (B/AT pairs) and born after a twin birth (AT siblings). For the genome-wide average DNA methylation, the correlation for AT pairs (r(AT)) was larger than the correlation for BT pairs (r(BT)) in both studies, and from the meta-analysis, r(AT) = 0.46 (95% CI: 0.26, 0.63) and r(BT) = −0.003 (95% CI: −0.30, 0.29) (P = 0.02). B/AT pairs were not correlated (from the meta-analysis r(BAT) = 0.08; 95% CI: −0.31, 0.45). Similar results were found for the average methylation of several genomic regions, e.g., CpG shelf and gene body. BT and AT pairs were differentially correlated in methylation for 15 probes (all P < 10(−7)), and the top 152 differentially correlated probes (at P < 10(−4)) were enriched in cell signalling and breast cancer regulation pathways. Our observations are consistent with a twin birth changing the intrauterine environment such that siblings both born after a twin birth are correlated in DNA methylation.
format Online
Article
Text
id pubmed-5559542
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-55595422017-08-18 Twin birth changes DNA methylation of subsequent siblings Li, Shuai Kim, Eunae Wong, Ee Ming Joo, Ji-Hoon Eric Nguyen, Tuong L. Stone, Jennifer Song, Yun-Mi Flander, Louisa B. Saffery, Richard Giles, Graham G. Southey, Melissa C. Sung, Joohon Hopper, John L. Sci Rep Article We asked if twin birth influences the DNA methylation of subsequent siblings. We measured whole blood methylation using the HumanMethylation450 array for siblings from two twin and family studies in Australia and Korea. We compared the means and correlations in methylation between pairs of siblings born before a twin birth (BT siblings), born on either side of a twin birth (B/AT pairs) and born after a twin birth (AT siblings). For the genome-wide average DNA methylation, the correlation for AT pairs (r(AT)) was larger than the correlation for BT pairs (r(BT)) in both studies, and from the meta-analysis, r(AT) = 0.46 (95% CI: 0.26, 0.63) and r(BT) = −0.003 (95% CI: −0.30, 0.29) (P = 0.02). B/AT pairs were not correlated (from the meta-analysis r(BAT) = 0.08; 95% CI: −0.31, 0.45). Similar results were found for the average methylation of several genomic regions, e.g., CpG shelf and gene body. BT and AT pairs were differentially correlated in methylation for 15 probes (all P < 10(−7)), and the top 152 differentially correlated probes (at P < 10(−4)) were enriched in cell signalling and breast cancer regulation pathways. Our observations are consistent with a twin birth changing the intrauterine environment such that siblings both born after a twin birth are correlated in DNA methylation. Nature Publishing Group UK 2017-08-16 /pmc/articles/PMC5559542/ /pubmed/28814741 http://dx.doi.org/10.1038/s41598-017-08595-6 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Li, Shuai
Kim, Eunae
Wong, Ee Ming
Joo, Ji-Hoon Eric
Nguyen, Tuong L.
Stone, Jennifer
Song, Yun-Mi
Flander, Louisa B.
Saffery, Richard
Giles, Graham G.
Southey, Melissa C.
Sung, Joohon
Hopper, John L.
Twin birth changes DNA methylation of subsequent siblings
title Twin birth changes DNA methylation of subsequent siblings
title_full Twin birth changes DNA methylation of subsequent siblings
title_fullStr Twin birth changes DNA methylation of subsequent siblings
title_full_unstemmed Twin birth changes DNA methylation of subsequent siblings
title_short Twin birth changes DNA methylation of subsequent siblings
title_sort twin birth changes dna methylation of subsequent siblings
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5559542/
https://www.ncbi.nlm.nih.gov/pubmed/28814741
http://dx.doi.org/10.1038/s41598-017-08595-6
work_keys_str_mv AT lishuai twinbirthchangesdnamethylationofsubsequentsiblings
AT kimeunae twinbirthchangesdnamethylationofsubsequentsiblings
AT wongeeming twinbirthchangesdnamethylationofsubsequentsiblings
AT joojihooneric twinbirthchangesdnamethylationofsubsequentsiblings
AT nguyentuongl twinbirthchangesdnamethylationofsubsequentsiblings
AT stonejennifer twinbirthchangesdnamethylationofsubsequentsiblings
AT songyunmi twinbirthchangesdnamethylationofsubsequentsiblings
AT flanderlouisab twinbirthchangesdnamethylationofsubsequentsiblings
AT safferyrichard twinbirthchangesdnamethylationofsubsequentsiblings
AT gilesgrahamg twinbirthchangesdnamethylationofsubsequentsiblings
AT southeymelissac twinbirthchangesdnamethylationofsubsequentsiblings
AT sungjoohon twinbirthchangesdnamethylationofsubsequentsiblings
AT hopperjohnl twinbirthchangesdnamethylationofsubsequentsiblings