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The [PSI(+)] yeast prion does not wildly affect proteome composition whereas selective pressure exerted on [PSI(+)] cells can promote aneuploidy
The yeast Sup35 protein is a subunit of the translation termination factor, and its conversion to the [PSI (+)] prion state leads to more translational read-through. Although extensive studies have been done on [PSI (+)], changes at the proteomic level have not been performed exhaustively. We theref...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5559586/ https://www.ncbi.nlm.nih.gov/pubmed/28814753 http://dx.doi.org/10.1038/s41598-017-07999-8 |
Sumario: | The yeast Sup35 protein is a subunit of the translation termination factor, and its conversion to the [PSI (+)] prion state leads to more translational read-through. Although extensive studies have been done on [PSI (+)], changes at the proteomic level have not been performed exhaustively. We therefore used a SILAC-based quantitative mass spectrometry approach and identified 4187 proteins from both [psi (−)] and [PSI (+)] strains. Surprisingly, there was very little difference between the two proteomes under standard growth conditions. We found however that several [PSI (+)] strains harbored an additional chromosome, such as chromosome I. Albeit, we found no evidence to support that [PSI (+)] induces chromosomal instability (CIN). Instead we hypothesized that the selective pressure applied during the establishment of [PSI (+)]-containing strains could lead to a supernumerary chromosome due to the presence of the ade1-14 selective marker for translational read-through. We therefore verified that there was no prevalence of disomy among newly generated [PSI (+)] strains in absence of strong selection pressure. We also noticed that low amounts of adenine in media could lead to higher levels of mitochondrial DNA in [PSI (+)] in ade1-14 cells. Our study has important significance for the establishment and manipulation of yeast strains with the Sup35 prion. |
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