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The [PSI(+)] yeast prion does not wildly affect proteome composition whereas selective pressure exerted on [PSI(+)] cells can promote aneuploidy

The yeast Sup35 protein is a subunit of the translation termination factor, and its conversion to the [PSI (+)] prion state leads to more translational read-through. Although extensive studies have been done on [PSI (+)], changes at the proteomic level have not been performed exhaustively. We theref...

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Detalles Bibliográficos
Autores principales: Chan, Patrick H. W., Lee, Lisa, Kim, Erin, Hui, Tony, Stoynov, Nikolay, Nassar, Roy, Moksa, Michelle, Cameron, Dale M., Hirst, Martin, Gsponer, Joerg, Mayor, Thibault
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5559586/
https://www.ncbi.nlm.nih.gov/pubmed/28814753
http://dx.doi.org/10.1038/s41598-017-07999-8
Descripción
Sumario:The yeast Sup35 protein is a subunit of the translation termination factor, and its conversion to the [PSI (+)] prion state leads to more translational read-through. Although extensive studies have been done on [PSI (+)], changes at the proteomic level have not been performed exhaustively. We therefore used a SILAC-based quantitative mass spectrometry approach and identified 4187 proteins from both [psi (−)] and [PSI (+)] strains. Surprisingly, there was very little difference between the two proteomes under standard growth conditions. We found however that several [PSI (+)] strains harbored an additional chromosome, such as chromosome I. Albeit, we found no evidence to support that [PSI (+)] induces chromosomal instability (CIN). Instead we hypothesized that the selective pressure applied during the establishment of [PSI (+)]-containing strains could lead to a supernumerary chromosome due to the presence of the ade1-14 selective marker for translational read-through. We therefore verified that there was no prevalence of disomy among newly generated [PSI (+)] strains in absence of strong selection pressure. We also noticed that low amounts of adenine in media could lead to higher levels of mitochondrial DNA in [PSI (+)] in ade1-14 cells. Our study has important significance for the establishment and manipulation of yeast strains with the Sup35 prion.