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Intracellular “activated” two-photon photodynamic therapy by fluorescent conveyor and photosensitizer co-encapsulating pH-responsive micelles against breast cancer

The application of photodynamic therapy (PDT) for the diagnosis and treatment of cancer is hindered by the intrinsic defects of the currently available photosensitizers (PSs), such as poor water solubility and limited light-penetration depth. In this study, pH-responsive polymeric micelles that co-e...

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Autores principales: Luo, Lei, Zhong, Hong, Liu, Shuang, Deng, Lidong, Luo, Yonghuang, Zhang, Qiong, Zhu, Yingzhong, Tian, Yupeng, Sun, Yuan, Tian, Xiaohe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5560416/
https://www.ncbi.nlm.nih.gov/pubmed/28860747
http://dx.doi.org/10.2147/IJN.S140345
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author Luo, Lei
Zhong, Hong
Liu, Shuang
Deng, Lidong
Luo, Yonghuang
Zhang, Qiong
Zhu, Yingzhong
Tian, Yupeng
Sun, Yuan
Tian, Xiaohe
author_facet Luo, Lei
Zhong, Hong
Liu, Shuang
Deng, Lidong
Luo, Yonghuang
Zhang, Qiong
Zhu, Yingzhong
Tian, Yupeng
Sun, Yuan
Tian, Xiaohe
author_sort Luo, Lei
collection PubMed
description The application of photodynamic therapy (PDT) for the diagnosis and treatment of cancer is hindered by the intrinsic defects of the currently available photosensitizers (PSs), such as poor water solubility and limited light-penetration depth. In this study, pH-responsive polymeric micelles that co-encapsulate therapeutic PSs and organooxotin two-photon compounds were applied for two-photon PDT (TP-PDT) against breast cancer. The TP-PDT effect of the drug-loaded micelles was “activated” when the micelles turned into aggregates at a triggering pH level. The in vitro therapeutic effect was evaluated on 4T1 murine breast cancer cells by viability assays, real-time morphology collapsing, and reactive oxygen species determination. Time-dependent ex vivo organ distribution and in vivo anticancer efficacy results suggested that the drug carriers could accumulate in tumors and suppress tumor growth by TP-PDT. The delivery system could enhance the solubility and distribution of PSs and, if administered along with a tissue-penetrating prolonged light source, could thus have good potential for cancer therapy.
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spelling pubmed-55604162017-08-31 Intracellular “activated” two-photon photodynamic therapy by fluorescent conveyor and photosensitizer co-encapsulating pH-responsive micelles against breast cancer Luo, Lei Zhong, Hong Liu, Shuang Deng, Lidong Luo, Yonghuang Zhang, Qiong Zhu, Yingzhong Tian, Yupeng Sun, Yuan Tian, Xiaohe Int J Nanomedicine Original Research The application of photodynamic therapy (PDT) for the diagnosis and treatment of cancer is hindered by the intrinsic defects of the currently available photosensitizers (PSs), such as poor water solubility and limited light-penetration depth. In this study, pH-responsive polymeric micelles that co-encapsulate therapeutic PSs and organooxotin two-photon compounds were applied for two-photon PDT (TP-PDT) against breast cancer. The TP-PDT effect of the drug-loaded micelles was “activated” when the micelles turned into aggregates at a triggering pH level. The in vitro therapeutic effect was evaluated on 4T1 murine breast cancer cells by viability assays, real-time morphology collapsing, and reactive oxygen species determination. Time-dependent ex vivo organ distribution and in vivo anticancer efficacy results suggested that the drug carriers could accumulate in tumors and suppress tumor growth by TP-PDT. The delivery system could enhance the solubility and distribution of PSs and, if administered along with a tissue-penetrating prolonged light source, could thus have good potential for cancer therapy. Dove Medical Press 2017-07-21 /pmc/articles/PMC5560416/ /pubmed/28860747 http://dx.doi.org/10.2147/IJN.S140345 Text en © 2017 Luo et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Luo, Lei
Zhong, Hong
Liu, Shuang
Deng, Lidong
Luo, Yonghuang
Zhang, Qiong
Zhu, Yingzhong
Tian, Yupeng
Sun, Yuan
Tian, Xiaohe
Intracellular “activated” two-photon photodynamic therapy by fluorescent conveyor and photosensitizer co-encapsulating pH-responsive micelles against breast cancer
title Intracellular “activated” two-photon photodynamic therapy by fluorescent conveyor and photosensitizer co-encapsulating pH-responsive micelles against breast cancer
title_full Intracellular “activated” two-photon photodynamic therapy by fluorescent conveyor and photosensitizer co-encapsulating pH-responsive micelles against breast cancer
title_fullStr Intracellular “activated” two-photon photodynamic therapy by fluorescent conveyor and photosensitizer co-encapsulating pH-responsive micelles against breast cancer
title_full_unstemmed Intracellular “activated” two-photon photodynamic therapy by fluorescent conveyor and photosensitizer co-encapsulating pH-responsive micelles against breast cancer
title_short Intracellular “activated” two-photon photodynamic therapy by fluorescent conveyor and photosensitizer co-encapsulating pH-responsive micelles against breast cancer
title_sort intracellular “activated” two-photon photodynamic therapy by fluorescent conveyor and photosensitizer co-encapsulating ph-responsive micelles against breast cancer
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5560416/
https://www.ncbi.nlm.nih.gov/pubmed/28860747
http://dx.doi.org/10.2147/IJN.S140345
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