Cargando…
Immunohistochemical analysis reveals variations in proteasome tissue expression in C. elegans
The ubiquitin-proteasome system (UPS) plays a crucial part in normal cell function by mediating intracellular protein clearance. We have previously shown that UPS-mediated protein degradation varies in a cell type-specific manner in C. elegans. Here, we use formalin-fixed, paraffin-embedded C. elega...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5560697/ https://www.ncbi.nlm.nih.gov/pubmed/28817671 http://dx.doi.org/10.1371/journal.pone.0183403 |
_version_ | 1783257706628382720 |
---|---|
author | Mikkonen, Elisa Haglund, Caj Holmberg, Carina I. |
author_facet | Mikkonen, Elisa Haglund, Caj Holmberg, Carina I. |
author_sort | Mikkonen, Elisa |
collection | PubMed |
description | The ubiquitin-proteasome system (UPS) plays a crucial part in normal cell function by mediating intracellular protein clearance. We have previously shown that UPS-mediated protein degradation varies in a cell type-specific manner in C. elegans. Here, we use formalin-fixed, paraffin-embedded C. elegans sections to enable studies on endogenous proteasome tissue expression. We show that the proteasome immunoreactivity pattern differs between cell types and within subcellular compartments in adult wild-type (N2) C. elegans. Interestingly, widespread knockdown of proteasome subunits by RNAi results in tissue-specific changes in proteasome expression instead of a uniform response. In addition, long-lived daf-2(e1370) mutants with impaired insulin/IGF-1 signaling (IIS) display similar proteasome tissue expression as aged-matched wild-type animals. Our study emphasizes the importance of alternate approaches to the commonly used whole animal lysate-based methods to detect changes in proteasome expression occurring at the sub-cellular, cell or tissue resolution level in a multicellular organism. |
format | Online Article Text |
id | pubmed-5560697 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-55606972017-08-25 Immunohistochemical analysis reveals variations in proteasome tissue expression in C. elegans Mikkonen, Elisa Haglund, Caj Holmberg, Carina I. PLoS One Research Article The ubiquitin-proteasome system (UPS) plays a crucial part in normal cell function by mediating intracellular protein clearance. We have previously shown that UPS-mediated protein degradation varies in a cell type-specific manner in C. elegans. Here, we use formalin-fixed, paraffin-embedded C. elegans sections to enable studies on endogenous proteasome tissue expression. We show that the proteasome immunoreactivity pattern differs between cell types and within subcellular compartments in adult wild-type (N2) C. elegans. Interestingly, widespread knockdown of proteasome subunits by RNAi results in tissue-specific changes in proteasome expression instead of a uniform response. In addition, long-lived daf-2(e1370) mutants with impaired insulin/IGF-1 signaling (IIS) display similar proteasome tissue expression as aged-matched wild-type animals. Our study emphasizes the importance of alternate approaches to the commonly used whole animal lysate-based methods to detect changes in proteasome expression occurring at the sub-cellular, cell or tissue resolution level in a multicellular organism. Public Library of Science 2017-08-17 /pmc/articles/PMC5560697/ /pubmed/28817671 http://dx.doi.org/10.1371/journal.pone.0183403 Text en © 2017 Mikkonen et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Mikkonen, Elisa Haglund, Caj Holmberg, Carina I. Immunohistochemical analysis reveals variations in proteasome tissue expression in C. elegans |
title | Immunohistochemical analysis reveals variations in proteasome tissue expression in C. elegans |
title_full | Immunohistochemical analysis reveals variations in proteasome tissue expression in C. elegans |
title_fullStr | Immunohistochemical analysis reveals variations in proteasome tissue expression in C. elegans |
title_full_unstemmed | Immunohistochemical analysis reveals variations in proteasome tissue expression in C. elegans |
title_short | Immunohistochemical analysis reveals variations in proteasome tissue expression in C. elegans |
title_sort | immunohistochemical analysis reveals variations in proteasome tissue expression in c. elegans |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5560697/ https://www.ncbi.nlm.nih.gov/pubmed/28817671 http://dx.doi.org/10.1371/journal.pone.0183403 |
work_keys_str_mv | AT mikkonenelisa immunohistochemicalanalysisrevealsvariationsinproteasometissueexpressionincelegans AT haglundcaj immunohistochemicalanalysisrevealsvariationsinproteasometissueexpressionincelegans AT holmbergcarinai immunohistochemicalanalysisrevealsvariationsinproteasometissueexpressionincelegans |