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Anti-biofilm effects of anthranilate on a broad range of bacteria

Anthranilate, one of tryptophan degradation products has been reported to interfere with biofilm formation by Pseudomonas aeruginosa. Here, we investigated the effects of anthranilate on biofilm formation by various bacteria and the mechanisms responsible. Anthranilate commonly inhibited biofilm for...

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Autores principales: Li, Xi-Hui, Kim, Soo-Kyoung, Lee, Joon-Hee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5561115/
https://www.ncbi.nlm.nih.gov/pubmed/28819217
http://dx.doi.org/10.1038/s41598-017-06540-1
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author Li, Xi-Hui
Kim, Soo-Kyoung
Lee, Joon-Hee
author_facet Li, Xi-Hui
Kim, Soo-Kyoung
Lee, Joon-Hee
author_sort Li, Xi-Hui
collection PubMed
description Anthranilate, one of tryptophan degradation products has been reported to interfere with biofilm formation by Pseudomonas aeruginosa. Here, we investigated the effects of anthranilate on biofilm formation by various bacteria and the mechanisms responsible. Anthranilate commonly inhibited biofilm formation by P. aeruginosa, Vibrio vulnificus, Bacillus subtilis, Salmonella enterica serovar Typhimurium, and Staphylococcus aureus, and disrupted biofilms preformed by these bacteria. Because anthranilate reduced intracellular c-di-GMP and enhanced swimming and swarming motilities in P. aeruginosa, V. vulnificus, B. subtilis, and S. enterica, it is likely that anthranilate disrupts biofilms by inducing the dispersion of these bacteria. On the other hand, in S. aureus, a non-flagellate bacterium that has no c-di-GMP signaling, anthranilate probably inhibits biofilm formation by reducing slime production. These results suggest that anthranilate has multiple ways for biofilm inhibition. Furthermore, because of its good biofilm inhibitory effects and lack of cytotoxicity to human cells even at high concentration, anthranilate appears to be a promising agent for inhibiting biofilm formation by a broad range of bacteria.
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spelling pubmed-55611152017-08-18 Anti-biofilm effects of anthranilate on a broad range of bacteria Li, Xi-Hui Kim, Soo-Kyoung Lee, Joon-Hee Sci Rep Article Anthranilate, one of tryptophan degradation products has been reported to interfere with biofilm formation by Pseudomonas aeruginosa. Here, we investigated the effects of anthranilate on biofilm formation by various bacteria and the mechanisms responsible. Anthranilate commonly inhibited biofilm formation by P. aeruginosa, Vibrio vulnificus, Bacillus subtilis, Salmonella enterica serovar Typhimurium, and Staphylococcus aureus, and disrupted biofilms preformed by these bacteria. Because anthranilate reduced intracellular c-di-GMP and enhanced swimming and swarming motilities in P. aeruginosa, V. vulnificus, B. subtilis, and S. enterica, it is likely that anthranilate disrupts biofilms by inducing the dispersion of these bacteria. On the other hand, in S. aureus, a non-flagellate bacterium that has no c-di-GMP signaling, anthranilate probably inhibits biofilm formation by reducing slime production. These results suggest that anthranilate has multiple ways for biofilm inhibition. Furthermore, because of its good biofilm inhibitory effects and lack of cytotoxicity to human cells even at high concentration, anthranilate appears to be a promising agent for inhibiting biofilm formation by a broad range of bacteria. Nature Publishing Group UK 2017-08-17 /pmc/articles/PMC5561115/ /pubmed/28819217 http://dx.doi.org/10.1038/s41598-017-06540-1 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Li, Xi-Hui
Kim, Soo-Kyoung
Lee, Joon-Hee
Anti-biofilm effects of anthranilate on a broad range of bacteria
title Anti-biofilm effects of anthranilate on a broad range of bacteria
title_full Anti-biofilm effects of anthranilate on a broad range of bacteria
title_fullStr Anti-biofilm effects of anthranilate on a broad range of bacteria
title_full_unstemmed Anti-biofilm effects of anthranilate on a broad range of bacteria
title_short Anti-biofilm effects of anthranilate on a broad range of bacteria
title_sort anti-biofilm effects of anthranilate on a broad range of bacteria
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5561115/
https://www.ncbi.nlm.nih.gov/pubmed/28819217
http://dx.doi.org/10.1038/s41598-017-06540-1
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