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Soft electroporation for delivering molecules into tightly adherent mammalian cells through 3D hollow nanoelectrodes
Electroporation of in-vitro cultured cells is widely used in biological and medical areas to deliver molecules of interest inside cells. Since very high electric fields are required to electroporate the plasma membrane, depending on the geometry of the electrodes the required voltages can be very hi...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5561120/ https://www.ncbi.nlm.nih.gov/pubmed/28819252 http://dx.doi.org/10.1038/s41598-017-08886-y |
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author | Caprettini, Valeria Cerea, Andrea Melle, Giovanni Lovato, Laura Capozza, Rosario Huang, Jian-An Tantussi, Francesco Dipalo, Michele De Angelis, Francesco |
author_facet | Caprettini, Valeria Cerea, Andrea Melle, Giovanni Lovato, Laura Capozza, Rosario Huang, Jian-An Tantussi, Francesco Dipalo, Michele De Angelis, Francesco |
author_sort | Caprettini, Valeria |
collection | PubMed |
description | Electroporation of in-vitro cultured cells is widely used in biological and medical areas to deliver molecules of interest inside cells. Since very high electric fields are required to electroporate the plasma membrane, depending on the geometry of the electrodes the required voltages can be very high and often critical to cell viability. Furthermore, in traditional electroporation configuration based on planar electrodes there is no a priori certain feedback about which cell has been targeted and delivered and the addition of fluorophores may be needed to gain this information. In this study we present a nanofabricated platform able to perform intracellular delivery of membrane-impermeable molecules by opening transient nanopores into the lipid membrane of adherent cells with high spatial precision and with the application of low voltages (1.5–2 V). This result is obtained by exploiting the tight seal that the cells present with 3D fluidic hollow gold-coated nanostructures that act as nanochannels and nanoelectrodes at the same time. The final soft-electroporation platform provides an accessible approach for controlled and selective drug delivery on ordered arrangements of cells. |
format | Online Article Text |
id | pubmed-5561120 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-55611202017-08-18 Soft electroporation for delivering molecules into tightly adherent mammalian cells through 3D hollow nanoelectrodes Caprettini, Valeria Cerea, Andrea Melle, Giovanni Lovato, Laura Capozza, Rosario Huang, Jian-An Tantussi, Francesco Dipalo, Michele De Angelis, Francesco Sci Rep Article Electroporation of in-vitro cultured cells is widely used in biological and medical areas to deliver molecules of interest inside cells. Since very high electric fields are required to electroporate the plasma membrane, depending on the geometry of the electrodes the required voltages can be very high and often critical to cell viability. Furthermore, in traditional electroporation configuration based on planar electrodes there is no a priori certain feedback about which cell has been targeted and delivered and the addition of fluorophores may be needed to gain this information. In this study we present a nanofabricated platform able to perform intracellular delivery of membrane-impermeable molecules by opening transient nanopores into the lipid membrane of adherent cells with high spatial precision and with the application of low voltages (1.5–2 V). This result is obtained by exploiting the tight seal that the cells present with 3D fluidic hollow gold-coated nanostructures that act as nanochannels and nanoelectrodes at the same time. The final soft-electroporation platform provides an accessible approach for controlled and selective drug delivery on ordered arrangements of cells. Nature Publishing Group UK 2017-08-17 /pmc/articles/PMC5561120/ /pubmed/28819252 http://dx.doi.org/10.1038/s41598-017-08886-y Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Caprettini, Valeria Cerea, Andrea Melle, Giovanni Lovato, Laura Capozza, Rosario Huang, Jian-An Tantussi, Francesco Dipalo, Michele De Angelis, Francesco Soft electroporation for delivering molecules into tightly adherent mammalian cells through 3D hollow nanoelectrodes |
title | Soft electroporation for delivering molecules into tightly adherent mammalian cells through 3D hollow nanoelectrodes |
title_full | Soft electroporation for delivering molecules into tightly adherent mammalian cells through 3D hollow nanoelectrodes |
title_fullStr | Soft electroporation for delivering molecules into tightly adherent mammalian cells through 3D hollow nanoelectrodes |
title_full_unstemmed | Soft electroporation for delivering molecules into tightly adherent mammalian cells through 3D hollow nanoelectrodes |
title_short | Soft electroporation for delivering molecules into tightly adherent mammalian cells through 3D hollow nanoelectrodes |
title_sort | soft electroporation for delivering molecules into tightly adherent mammalian cells through 3d hollow nanoelectrodes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5561120/ https://www.ncbi.nlm.nih.gov/pubmed/28819252 http://dx.doi.org/10.1038/s41598-017-08886-y |
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