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A single-molecule assessment of the protective effect of DMSO against DNA double-strand breaks induced by photo-and γ-ray-irradiation, and freezing

Dimethyl sulfoxide (DMSO) is widely used as a cryoprotectant for organs, tissues, and cell suspension in storage. In addition, DMSO is known to be a useful free radical scavenger and a radio-protectant. To date, many in vitro assays using cultured cells have been performed for analysing the protecti...

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Autores principales: Noda, Masami, Ma, Yue, Yoshikawa, Yuko, Imanaka, Tadayuki, Mori, Toshiaki, Furuta, Masakazu, Tsuruyama, Tatsuaki, Yoshikawa, Kenichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5561226/
https://www.ncbi.nlm.nih.gov/pubmed/28819291
http://dx.doi.org/10.1038/s41598-017-08894-y
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author Noda, Masami
Ma, Yue
Yoshikawa, Yuko
Imanaka, Tadayuki
Mori, Toshiaki
Furuta, Masakazu
Tsuruyama, Tatsuaki
Yoshikawa, Kenichi
author_facet Noda, Masami
Ma, Yue
Yoshikawa, Yuko
Imanaka, Tadayuki
Mori, Toshiaki
Furuta, Masakazu
Tsuruyama, Tatsuaki
Yoshikawa, Kenichi
author_sort Noda, Masami
collection PubMed
description Dimethyl sulfoxide (DMSO) is widely used as a cryoprotectant for organs, tissues, and cell suspension in storage. In addition, DMSO is known to be a useful free radical scavenger and a radio-protectant. To date, many in vitro assays using cultured cells have been performed for analysing the protective effect of DMSO against genomic DNA damage; however, currently it has been rather difficult to detect DNA double strand breaks (DSBs) in a quantitative manner. In the present study, we aimed to observe the extent of DNA damage by use of single molecular observation with a fluorescence microscope to evaluate DSBs induced by photo- and γ-ray-irradiation, or freeze/thawing in variable concentrations of DMSO. As a result, we found that 2% DMSO conferred the maximum protective effect against all of the injury sources tested, and these effects were maintained at higher concentrations. Further, DMSO showed a significantly higher protective effect against freezing-induced damage than against photo- and γ-ray-irradiation-induced damage. Our study provides significant data for the optimization of DNA cryopreservation with DMSO, as well as for the usage of DNA as the protective agent against the injuries caused by active oxygen and radiations.
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spelling pubmed-55612262017-08-21 A single-molecule assessment of the protective effect of DMSO against DNA double-strand breaks induced by photo-and γ-ray-irradiation, and freezing Noda, Masami Ma, Yue Yoshikawa, Yuko Imanaka, Tadayuki Mori, Toshiaki Furuta, Masakazu Tsuruyama, Tatsuaki Yoshikawa, Kenichi Sci Rep Article Dimethyl sulfoxide (DMSO) is widely used as a cryoprotectant for organs, tissues, and cell suspension in storage. In addition, DMSO is known to be a useful free radical scavenger and a radio-protectant. To date, many in vitro assays using cultured cells have been performed for analysing the protective effect of DMSO against genomic DNA damage; however, currently it has been rather difficult to detect DNA double strand breaks (DSBs) in a quantitative manner. In the present study, we aimed to observe the extent of DNA damage by use of single molecular observation with a fluorescence microscope to evaluate DSBs induced by photo- and γ-ray-irradiation, or freeze/thawing in variable concentrations of DMSO. As a result, we found that 2% DMSO conferred the maximum protective effect against all of the injury sources tested, and these effects were maintained at higher concentrations. Further, DMSO showed a significantly higher protective effect against freezing-induced damage than against photo- and γ-ray-irradiation-induced damage. Our study provides significant data for the optimization of DNA cryopreservation with DMSO, as well as for the usage of DNA as the protective agent against the injuries caused by active oxygen and radiations. Nature Publishing Group UK 2017-08-17 /pmc/articles/PMC5561226/ /pubmed/28819291 http://dx.doi.org/10.1038/s41598-017-08894-y Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Noda, Masami
Ma, Yue
Yoshikawa, Yuko
Imanaka, Tadayuki
Mori, Toshiaki
Furuta, Masakazu
Tsuruyama, Tatsuaki
Yoshikawa, Kenichi
A single-molecule assessment of the protective effect of DMSO against DNA double-strand breaks induced by photo-and γ-ray-irradiation, and freezing
title A single-molecule assessment of the protective effect of DMSO against DNA double-strand breaks induced by photo-and γ-ray-irradiation, and freezing
title_full A single-molecule assessment of the protective effect of DMSO against DNA double-strand breaks induced by photo-and γ-ray-irradiation, and freezing
title_fullStr A single-molecule assessment of the protective effect of DMSO against DNA double-strand breaks induced by photo-and γ-ray-irradiation, and freezing
title_full_unstemmed A single-molecule assessment of the protective effect of DMSO against DNA double-strand breaks induced by photo-and γ-ray-irradiation, and freezing
title_short A single-molecule assessment of the protective effect of DMSO against DNA double-strand breaks induced by photo-and γ-ray-irradiation, and freezing
title_sort single-molecule assessment of the protective effect of dmso against dna double-strand breaks induced by photo-and γ-ray-irradiation, and freezing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5561226/
https://www.ncbi.nlm.nih.gov/pubmed/28819291
http://dx.doi.org/10.1038/s41598-017-08894-y
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