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Levobuipivacaine-Induced Dissemination of A549 Lung Cancer Cells

While anaesthetics are frequently used on cancer patients during surgical procedures, their consequence on cancer progression remains to be elucidated. In this study, we sought to investigate the influence of local anesthetics on lung cancer cell dissemination in vitro and in vivo. A549 human non-sm...

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Autores principales: Chan, Shun-Ming, Lin, Bo-Feng, Wong, Chih-Shung, Chuang, Wen-Ting, Chou, Yu-Ting, Wu, Zhi-Fu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5561232/
https://www.ncbi.nlm.nih.gov/pubmed/28819223
http://dx.doi.org/10.1038/s41598-017-08885-z
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author Chan, Shun-Ming
Lin, Bo-Feng
Wong, Chih-Shung
Chuang, Wen-Ting
Chou, Yu-Ting
Wu, Zhi-Fu
author_facet Chan, Shun-Ming
Lin, Bo-Feng
Wong, Chih-Shung
Chuang, Wen-Ting
Chou, Yu-Ting
Wu, Zhi-Fu
author_sort Chan, Shun-Ming
collection PubMed
description While anaesthetics are frequently used on cancer patients during surgical procedures, their consequence on cancer progression remains to be elucidated. In this study, we sought to investigate the influence of local anesthetics on lung cancer cell dissemination in vitro and in vivo. A549 human non-small lung cancer cells were treated with various local anaesthetics including ropivacaine, lidocaine, levobupivacaine and bupivacaine. Cell barrier property was assessed using an electric cell-substrate impedance sensing (ECIS) system. The epithelial-to-mesenchymal transition (EMT) of treated cells was studied by immunofluorescence staining. In vitro and in vivo cancer cell dissemination were investigated.Gene expression microarray and quantitative real-time PCR (qrt-PCR) assays were used to identify the genes responsible for levobupivacaine-mediated cancer cell dissemination.The results illustrated that only levobupivacaine induced EMT in the treated cells and also caused the dissemination of cancer cells in vitro. In addition, after intravenous injection, levobupivacaine encouraged cancer cell dissemination in vivo. Gene expression microarray, qrt-PCR and immunoblotting revealed that after levobupivacaine treatment, the hypoxia-inducible factor (HIF)- 2α gene was upregulated in cancer cells. Our findings suggest that levobupivacaine may induce A549 lung cancer cell dissemination both in vitro and in vivo. More specifically, HIF-2α signaling possibly contributes to levobupivacaine-mediated A549 lung cancer cell dissemination.
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spelling pubmed-55612322017-08-21 Levobuipivacaine-Induced Dissemination of A549 Lung Cancer Cells Chan, Shun-Ming Lin, Bo-Feng Wong, Chih-Shung Chuang, Wen-Ting Chou, Yu-Ting Wu, Zhi-Fu Sci Rep Article While anaesthetics are frequently used on cancer patients during surgical procedures, their consequence on cancer progression remains to be elucidated. In this study, we sought to investigate the influence of local anesthetics on lung cancer cell dissemination in vitro and in vivo. A549 human non-small lung cancer cells were treated with various local anaesthetics including ropivacaine, lidocaine, levobupivacaine and bupivacaine. Cell barrier property was assessed using an electric cell-substrate impedance sensing (ECIS) system. The epithelial-to-mesenchymal transition (EMT) of treated cells was studied by immunofluorescence staining. In vitro and in vivo cancer cell dissemination were investigated.Gene expression microarray and quantitative real-time PCR (qrt-PCR) assays were used to identify the genes responsible for levobupivacaine-mediated cancer cell dissemination.The results illustrated that only levobupivacaine induced EMT in the treated cells and also caused the dissemination of cancer cells in vitro. In addition, after intravenous injection, levobupivacaine encouraged cancer cell dissemination in vivo. Gene expression microarray, qrt-PCR and immunoblotting revealed that after levobupivacaine treatment, the hypoxia-inducible factor (HIF)- 2α gene was upregulated in cancer cells. Our findings suggest that levobupivacaine may induce A549 lung cancer cell dissemination both in vitro and in vivo. More specifically, HIF-2α signaling possibly contributes to levobupivacaine-mediated A549 lung cancer cell dissemination. Nature Publishing Group UK 2017-08-17 /pmc/articles/PMC5561232/ /pubmed/28819223 http://dx.doi.org/10.1038/s41598-017-08885-z Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Chan, Shun-Ming
Lin, Bo-Feng
Wong, Chih-Shung
Chuang, Wen-Ting
Chou, Yu-Ting
Wu, Zhi-Fu
Levobuipivacaine-Induced Dissemination of A549 Lung Cancer Cells
title Levobuipivacaine-Induced Dissemination of A549 Lung Cancer Cells
title_full Levobuipivacaine-Induced Dissemination of A549 Lung Cancer Cells
title_fullStr Levobuipivacaine-Induced Dissemination of A549 Lung Cancer Cells
title_full_unstemmed Levobuipivacaine-Induced Dissemination of A549 Lung Cancer Cells
title_short Levobuipivacaine-Induced Dissemination of A549 Lung Cancer Cells
title_sort levobuipivacaine-induced dissemination of a549 lung cancer cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5561232/
https://www.ncbi.nlm.nih.gov/pubmed/28819223
http://dx.doi.org/10.1038/s41598-017-08885-z
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