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CRISPR-Cas12a-Assisted Recombineering in Bacteria

Clustered regularly interspaced short palindromic repeat (CRISPR)-Cas12a (Cpf1) has emerged as an effective genome editing tool in many organisms. Here, we developed and optimized a CRISPR-Cas12a-assisted recombineering system to facilitate genetic manipulation in bacteria. Using this system, point...

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Detalles Bibliográficos
Autores principales:	Yan, Mei-Yi, Yan, Hai-Qin, Ren, Gai-Xian, Zhao, Ju-Ping, Guo, Xiao-Peng, Sun, Yi-Cheng
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Society for Microbiology 2017
Materias:	Methods
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5561284/ https://www.ncbi.nlm.nih.gov/pubmed/28646112 http://dx.doi.org/10.1128/AEM.00947-17

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