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Isolation of rat Schwann cells based on cell sorting
The present study presented a protocol that can be used to obtain rapidly a high purity of proliferating rat Schwann cells from freshly dissociated rat peripheral nerves. The sciatic nerves of newborn rats (1–3 day old) were dissociated, and the Schwann cells (SCs) were purified using fluorescence-a...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5561794/ https://www.ncbi.nlm.nih.gov/pubmed/28627700 http://dx.doi.org/10.3892/mmr.2017.6777 |
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author | Shen, Mi Tang, Wei Cao, Zheng Cao, Xuemin Ding, Fei |
author_facet | Shen, Mi Tang, Wei Cao, Zheng Cao, Xuemin Ding, Fei |
author_sort | Shen, Mi |
collection | PubMed |
description | The present study presented a protocol that can be used to obtain rapidly a high purity of proliferating rat Schwann cells from freshly dissociated rat peripheral nerves. The sciatic nerves of newborn rats (1–3 day old) were dissociated, and the Schwann cells (SCs) were purified using fluorescence-activated cell sorting (FACS) based on the SC membrane-specific expression of the low-affinity nerve growth factor receptor, p75NGFR and oligodendrocyte marker 4. Following sorting, the cells were plated on poly-l-lysine-coated dishes in SC culture medium containing DMEM with 10% FBS, 1% penicillin/streptomycin, 2 µM forskolin and 10 ng/ml HRG. The purified rat SCs were propagated for passaging until confluent. This protocol resulted in SC cultures, which were >98% pure. This FACS-based protocol can be used to facilitate future investigations of general SC biology. |
format | Online Article Text |
id | pubmed-5561794 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-55617942017-10-23 Isolation of rat Schwann cells based on cell sorting Shen, Mi Tang, Wei Cao, Zheng Cao, Xuemin Ding, Fei Mol Med Rep Articles The present study presented a protocol that can be used to obtain rapidly a high purity of proliferating rat Schwann cells from freshly dissociated rat peripheral nerves. The sciatic nerves of newborn rats (1–3 day old) were dissociated, and the Schwann cells (SCs) were purified using fluorescence-activated cell sorting (FACS) based on the SC membrane-specific expression of the low-affinity nerve growth factor receptor, p75NGFR and oligodendrocyte marker 4. Following sorting, the cells were plated on poly-l-lysine-coated dishes in SC culture medium containing DMEM with 10% FBS, 1% penicillin/streptomycin, 2 µM forskolin and 10 ng/ml HRG. The purified rat SCs were propagated for passaging until confluent. This protocol resulted in SC cultures, which were >98% pure. This FACS-based protocol can be used to facilitate future investigations of general SC biology. D.A. Spandidos 2017-08 2017-06-14 /pmc/articles/PMC5561794/ /pubmed/28627700 http://dx.doi.org/10.3892/mmr.2017.6777 Text en Copyright: © Shen et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Shen, Mi Tang, Wei Cao, Zheng Cao, Xuemin Ding, Fei Isolation of rat Schwann cells based on cell sorting |
title | Isolation of rat Schwann cells based on cell sorting |
title_full | Isolation of rat Schwann cells based on cell sorting |
title_fullStr | Isolation of rat Schwann cells based on cell sorting |
title_full_unstemmed | Isolation of rat Schwann cells based on cell sorting |
title_short | Isolation of rat Schwann cells based on cell sorting |
title_sort | isolation of rat schwann cells based on cell sorting |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5561794/ https://www.ncbi.nlm.nih.gov/pubmed/28627700 http://dx.doi.org/10.3892/mmr.2017.6777 |
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