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Effect of canine cortical bone demineralization on osteogenic differentiation of adipose-derived mesenchymal stromal cells

Demineralized bone allografts and mesenchymal stromal cells have been used to promote bone regeneration. However, the degree to which cortical bone should be demineralized for use in combination with adipose-derived mesenchymal stromal cells (Ad-MSCs) remains to be clarified. In this study, the in v...

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Autores principales: Jo, Kwangrae, Kim, Yongsun, Lee, Seung Hoon, Yoon, Yong Seok, Kim, Wan Hee, Kweon, Oh-Kyeong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5561973/
https://www.ncbi.nlm.nih.gov/pubmed/28856336
http://dx.doi.org/10.1016/j.heliyon.2017.e00383
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author Jo, Kwangrae
Kim, Yongsun
Lee, Seung Hoon
Yoon, Yong Seok
Kim, Wan Hee
Kweon, Oh-Kyeong
author_facet Jo, Kwangrae
Kim, Yongsun
Lee, Seung Hoon
Yoon, Yong Seok
Kim, Wan Hee
Kweon, Oh-Kyeong
author_sort Jo, Kwangrae
collection PubMed
description Demineralized bone allografts and mesenchymal stromal cells have been used to promote bone regeneration. However, the degree to which cortical bone should be demineralized for use in combination with adipose-derived mesenchymal stromal cells (Ad-MSCs) remains to be clarified. In this study, the in vitro osteogenic ability of Ad-MSCs on allografts was investigated in relation to the extent of demineralization. Three treatment groups were established by varying exposure time to 0.6 N HCL: partially demineralized (PDB; 12 h), fully demineralized (FDB; 48 h), and non-demineralized bone (NDB; 0 h, as a control). Allografts were prepared as discs 6 mm in diameter for in vitro evaluation, and their demineralization and structure were evaluated by micro-computed tomography and scanning electron microscopy. Ad-MSC adhesion and proliferation were measured by MTS assay, and osteogenesis-related gene expression was assessed by quantitative reverse transcription polymerase chain reaction. PDB and FDB demineralization rates were 57.13 and 92.30%, respectively. Moreover, Ad-MSC adhesion rates on NDB, PDB, and FDB were 53.41, 60.65, and 61.32%, respectively. Proliferation of these cells on FDB increased significantly after 2 days of culture compared to the other groups (P < 0.05). Furthermore, expression of the osteogenic genes ALP, BMP-7, and TGF-β in the FDB group on culture day 3 was significantly elevated in comparison to the other treatments. Given its biocompatibility and promotion of the osteogenic differentiation of Ad-MSCs, our results suggest that FDB may be a suitable scaffold for use in the repair of bone defects.
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spelling pubmed-55619732017-08-30 Effect of canine cortical bone demineralization on osteogenic differentiation of adipose-derived mesenchymal stromal cells Jo, Kwangrae Kim, Yongsun Lee, Seung Hoon Yoon, Yong Seok Kim, Wan Hee Kweon, Oh-Kyeong Heliyon Article Demineralized bone allografts and mesenchymal stromal cells have been used to promote bone regeneration. However, the degree to which cortical bone should be demineralized for use in combination with adipose-derived mesenchymal stromal cells (Ad-MSCs) remains to be clarified. In this study, the in vitro osteogenic ability of Ad-MSCs on allografts was investigated in relation to the extent of demineralization. Three treatment groups were established by varying exposure time to 0.6 N HCL: partially demineralized (PDB; 12 h), fully demineralized (FDB; 48 h), and non-demineralized bone (NDB; 0 h, as a control). Allografts were prepared as discs 6 mm in diameter for in vitro evaluation, and their demineralization and structure were evaluated by micro-computed tomography and scanning electron microscopy. Ad-MSC adhesion and proliferation were measured by MTS assay, and osteogenesis-related gene expression was assessed by quantitative reverse transcription polymerase chain reaction. PDB and FDB demineralization rates were 57.13 and 92.30%, respectively. Moreover, Ad-MSC adhesion rates on NDB, PDB, and FDB were 53.41, 60.65, and 61.32%, respectively. Proliferation of these cells on FDB increased significantly after 2 days of culture compared to the other groups (P < 0.05). Furthermore, expression of the osteogenic genes ALP, BMP-7, and TGF-β in the FDB group on culture day 3 was significantly elevated in comparison to the other treatments. Given its biocompatibility and promotion of the osteogenic differentiation of Ad-MSCs, our results suggest that FDB may be a suitable scaffold for use in the repair of bone defects. Elsevier 2017-08-17 /pmc/articles/PMC5561973/ /pubmed/28856336 http://dx.doi.org/10.1016/j.heliyon.2017.e00383 Text en © 2017 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jo, Kwangrae
Kim, Yongsun
Lee, Seung Hoon
Yoon, Yong Seok
Kim, Wan Hee
Kweon, Oh-Kyeong
Effect of canine cortical bone demineralization on osteogenic differentiation of adipose-derived mesenchymal stromal cells
title Effect of canine cortical bone demineralization on osteogenic differentiation of adipose-derived mesenchymal stromal cells
title_full Effect of canine cortical bone demineralization on osteogenic differentiation of adipose-derived mesenchymal stromal cells
title_fullStr Effect of canine cortical bone demineralization on osteogenic differentiation of adipose-derived mesenchymal stromal cells
title_full_unstemmed Effect of canine cortical bone demineralization on osteogenic differentiation of adipose-derived mesenchymal stromal cells
title_short Effect of canine cortical bone demineralization on osteogenic differentiation of adipose-derived mesenchymal stromal cells
title_sort effect of canine cortical bone demineralization on osteogenic differentiation of adipose-derived mesenchymal stromal cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5561973/
https://www.ncbi.nlm.nih.gov/pubmed/28856336
http://dx.doi.org/10.1016/j.heliyon.2017.e00383
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