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Role of glycoprotein 78 and cidec in hepatic steatosis

Hepatic glycoprotein (gp78), a membrane-anchored E3 ubiquitin ligase, has been reported to be involved in regulating lipid and energy metabolism in animals, and cell death-inducing DFFA-like effector c (cidec) has emerged as an important regulator of metabolism, which has been implicated in the proc...

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Autores principales: Li, Jie, Liu, Guocai, Zhang, Feng, Zhang, Zhiwen, Xu, Yuqiao, Li, Qing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5561988/
https://www.ncbi.nlm.nih.gov/pubmed/28656280
http://dx.doi.org/10.3892/mmr.2017.6834
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author Li, Jie
Liu, Guocai
Zhang, Feng
Zhang, Zhiwen
Xu, Yuqiao
Li, Qing
author_facet Li, Jie
Liu, Guocai
Zhang, Feng
Zhang, Zhiwen
Xu, Yuqiao
Li, Qing
author_sort Li, Jie
collection PubMed
description Hepatic glycoprotein (gp78), a membrane-anchored E3 ubiquitin ligase, has been reported to be involved in regulating lipid and energy metabolism in animals, and cell death-inducing DFFA-like effector c (cidec) has emerged as an important regulator of metabolism, which has been implicated in the process of fat differentiation. Nonalcoholic fatty liver disease is a metabolic disorder associated with hepatic steatosis. In the present study, to investigate the role of gp78 and cidec in hepatic steatosis, an in vitro cell culture model of hepatic steatosis was established, using the AML12 mouse hepatocyte cell line to assess the protein expression of gp78. The results of Oil Red O staining, phase contrast microscopy and triglyceride content detection experiments indicated that the overexpression of gp78 induced lipid accumulation, whereas gp78-knockdown led to a reduction in lipid accumulation in the AML12 cells. The increased expression of gp78 was associated with steatosis. The expression of cidec was consistent with gp78, and the colocalization of gp78 and cidec was observed on the surface of lipid droplets using immunofluorescence analysis. Furthermore, an interaction between gp78 and cidec was detected using coimmunoprecipitation analysis, and this interaction promoted lipid accumulation. Based on these data, it was hypothesized that gp78 is a regulator of hepatic steatosis, and that it may be a putative molecular mediator in metabolic diseases.
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spelling pubmed-55619882017-10-23 Role of glycoprotein 78 and cidec in hepatic steatosis Li, Jie Liu, Guocai Zhang, Feng Zhang, Zhiwen Xu, Yuqiao Li, Qing Mol Med Rep Articles Hepatic glycoprotein (gp78), a membrane-anchored E3 ubiquitin ligase, has been reported to be involved in regulating lipid and energy metabolism in animals, and cell death-inducing DFFA-like effector c (cidec) has emerged as an important regulator of metabolism, which has been implicated in the process of fat differentiation. Nonalcoholic fatty liver disease is a metabolic disorder associated with hepatic steatosis. In the present study, to investigate the role of gp78 and cidec in hepatic steatosis, an in vitro cell culture model of hepatic steatosis was established, using the AML12 mouse hepatocyte cell line to assess the protein expression of gp78. The results of Oil Red O staining, phase contrast microscopy and triglyceride content detection experiments indicated that the overexpression of gp78 induced lipid accumulation, whereas gp78-knockdown led to a reduction in lipid accumulation in the AML12 cells. The increased expression of gp78 was associated with steatosis. The expression of cidec was consistent with gp78, and the colocalization of gp78 and cidec was observed on the surface of lipid droplets using immunofluorescence analysis. Furthermore, an interaction between gp78 and cidec was detected using coimmunoprecipitation analysis, and this interaction promoted lipid accumulation. Based on these data, it was hypothesized that gp78 is a regulator of hepatic steatosis, and that it may be a putative molecular mediator in metabolic diseases. D.A. Spandidos 2017-08 2017-06-21 /pmc/articles/PMC5561988/ /pubmed/28656280 http://dx.doi.org/10.3892/mmr.2017.6834 Text en Copyright: © Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Li, Jie
Liu, Guocai
Zhang, Feng
Zhang, Zhiwen
Xu, Yuqiao
Li, Qing
Role of glycoprotein 78 and cidec in hepatic steatosis
title Role of glycoprotein 78 and cidec in hepatic steatosis
title_full Role of glycoprotein 78 and cidec in hepatic steatosis
title_fullStr Role of glycoprotein 78 and cidec in hepatic steatosis
title_full_unstemmed Role of glycoprotein 78 and cidec in hepatic steatosis
title_short Role of glycoprotein 78 and cidec in hepatic steatosis
title_sort role of glycoprotein 78 and cidec in hepatic steatosis
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5561988/
https://www.ncbi.nlm.nih.gov/pubmed/28656280
http://dx.doi.org/10.3892/mmr.2017.6834
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