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Microarray analysis reveals differentially expressed lncRNAs in benign epithelial ovarian cysts and normal ovaries

Recent studies suggest that long non-coding RNAs (lncRNAs) play crucial roles in many types of human malignant cancers. However, the function of lncRNAs in benign tumors remains poorly understood. In the present study, to explored the potential roles of lncRNAs in benign epithelial ovarian cysts (BE...

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Autores principales: Liu, Xiaoguang, Dai, Chencheng, Jia, Genmei, Xu, Sujuan, Fu, Ziyi, Xu, Juan, Li, Qing, Ruan, Hongjie, Xu, Pengfei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5562051/
https://www.ncbi.nlm.nih.gov/pubmed/28656240
http://dx.doi.org/10.3892/or.2017.5741
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author Liu, Xiaoguang
Dai, Chencheng
Jia, Genmei
Xu, Sujuan
Fu, Ziyi
Xu, Juan
Li, Qing
Ruan, Hongjie
Xu, Pengfei
author_facet Liu, Xiaoguang
Dai, Chencheng
Jia, Genmei
Xu, Sujuan
Fu, Ziyi
Xu, Juan
Li, Qing
Ruan, Hongjie
Xu, Pengfei
author_sort Liu, Xiaoguang
collection PubMed
description Recent studies suggest that long non-coding RNAs (lncRNAs) play crucial roles in many types of human malignant cancers. However, the function of lncRNAs in benign tumors remains poorly understood. In the present study, to explored the potential roles of lncRNAs in benign epithelial ovarian cysts (BEOCs) which commonly occur in young women and possess malignant potential, we described the expression profile of the lncRNAs between BEOC and normal ovarian tissues using lncRNA microarray techniques. The results showed that 1,325 transcripts of lncRNAs (1,014 upregulated and 311 downregulated) were differentially expressed in the BEOCs compared with the normal controls [absolute fold-change ≥2, false discovery rate (FDR) <0.05]. We also conducted quantitative real-time PCR (qPCR) to confirm the microarray data. The results of qPCR revealed that the expression trend of 6 randomly selected lncRNAs was consistent with the microarray data. Furthermore, candidate lncRNAs were characterized by pathway analysis and Gene Ontology (GO). The present study is the first to demonstrate different expression profiles of lncRNAs between BEOCs and normal ovarian tissues. These lncRNAs may play a crucial role in the pathological process of BEOCs.
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spelling pubmed-55620512017-11-02 Microarray analysis reveals differentially expressed lncRNAs in benign epithelial ovarian cysts and normal ovaries Liu, Xiaoguang Dai, Chencheng Jia, Genmei Xu, Sujuan Fu, Ziyi Xu, Juan Li, Qing Ruan, Hongjie Xu, Pengfei Oncol Rep Articles Recent studies suggest that long non-coding RNAs (lncRNAs) play crucial roles in many types of human malignant cancers. However, the function of lncRNAs in benign tumors remains poorly understood. In the present study, to explored the potential roles of lncRNAs in benign epithelial ovarian cysts (BEOCs) which commonly occur in young women and possess malignant potential, we described the expression profile of the lncRNAs between BEOC and normal ovarian tissues using lncRNA microarray techniques. The results showed that 1,325 transcripts of lncRNAs (1,014 upregulated and 311 downregulated) were differentially expressed in the BEOCs compared with the normal controls [absolute fold-change ≥2, false discovery rate (FDR) <0.05]. We also conducted quantitative real-time PCR (qPCR) to confirm the microarray data. The results of qPCR revealed that the expression trend of 6 randomly selected lncRNAs was consistent with the microarray data. Furthermore, candidate lncRNAs were characterized by pathway analysis and Gene Ontology (GO). The present study is the first to demonstrate different expression profiles of lncRNAs between BEOCs and normal ovarian tissues. These lncRNAs may play a crucial role in the pathological process of BEOCs. D.A. Spandidos 2017-08 2017-06-22 /pmc/articles/PMC5562051/ /pubmed/28656240 http://dx.doi.org/10.3892/or.2017.5741 Text en Copyright: © Liu et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Liu, Xiaoguang
Dai, Chencheng
Jia, Genmei
Xu, Sujuan
Fu, Ziyi
Xu, Juan
Li, Qing
Ruan, Hongjie
Xu, Pengfei
Microarray analysis reveals differentially expressed lncRNAs in benign epithelial ovarian cysts and normal ovaries
title Microarray analysis reveals differentially expressed lncRNAs in benign epithelial ovarian cysts and normal ovaries
title_full Microarray analysis reveals differentially expressed lncRNAs in benign epithelial ovarian cysts and normal ovaries
title_fullStr Microarray analysis reveals differentially expressed lncRNAs in benign epithelial ovarian cysts and normal ovaries
title_full_unstemmed Microarray analysis reveals differentially expressed lncRNAs in benign epithelial ovarian cysts and normal ovaries
title_short Microarray analysis reveals differentially expressed lncRNAs in benign epithelial ovarian cysts and normal ovaries
title_sort microarray analysis reveals differentially expressed lncrnas in benign epithelial ovarian cysts and normal ovaries
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5562051/
https://www.ncbi.nlm.nih.gov/pubmed/28656240
http://dx.doi.org/10.3892/or.2017.5741
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