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Jak2a regulates erythroid and myeloid hematopoiesis during zebrafish embryogenesis
Zebrafish (Danio rerio) are an attractive vertebrate model for the molecular dissection of disease mechanisms. Janus kinase (JAK)/signal transducer and activator of transcription (stat) has been defined through studies of cytokine signaling pathways in mammals. Here, we examined the expression level...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Ivyspring International Publisher
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5562130/ https://www.ncbi.nlm.nih.gov/pubmed/28824311 http://dx.doi.org/10.7150/ijms.18907 |
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author | Zhu, Xianmin Liu, Rui Guan, Jun Zeng, Wen Yin, Jin Zhang, Yicheng |
author_facet | Zhu, Xianmin Liu, Rui Guan, Jun Zeng, Wen Yin, Jin Zhang, Yicheng |
author_sort | Zhu, Xianmin |
collection | PubMed |
description | Zebrafish (Danio rerio) are an attractive vertebrate model for the molecular dissection of disease mechanisms. Janus kinase (JAK)/signal transducer and activator of transcription (stat) has been defined through studies of cytokine signaling pathways in mammals. Here, we examined the expression level of Jak2a, which is a homolog of mammalian jak2 in zebrafish, by quantitative reverse transcriptase (RT)-PCR, and the peak of mRNA expression occurred at 3.75 hours post fertilization (hpf). The overexpression of Jak2a was proven by real-time Q-PCR and Western blot in 1-4-cell stage embryos injected with 400 ng/µl full-length jak2a mRNA as well as gfi1.1, gata1, mpo and β-embryonic hemoglobin as detected by real-time Q-PCR. Moreover, jak2a mRNA significantly increased the GFP(+) population in the transgenic zebrafish lines Tg (gata1: gfp) (uninjected embryos: 17.22±1.70%; embryos injected with jak2a mRNA: 21.31±2.11%, p<0.01) and Tg (mpo: gfp) (uninjected embryos: 3.86±1.94; embryos injected with jak2a mRNA: 6.64±1.30%, p<0.01) compared with the control group. Thus, our data indicate that Jak2a plays an important role in erythropoiesis and myeloid hematopoiesis. |
format | Online Article Text |
id | pubmed-5562130 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-55621302017-08-18 Jak2a regulates erythroid and myeloid hematopoiesis during zebrafish embryogenesis Zhu, Xianmin Liu, Rui Guan, Jun Zeng, Wen Yin, Jin Zhang, Yicheng Int J Med Sci Research Paper Zebrafish (Danio rerio) are an attractive vertebrate model for the molecular dissection of disease mechanisms. Janus kinase (JAK)/signal transducer and activator of transcription (stat) has been defined through studies of cytokine signaling pathways in mammals. Here, we examined the expression level of Jak2a, which is a homolog of mammalian jak2 in zebrafish, by quantitative reverse transcriptase (RT)-PCR, and the peak of mRNA expression occurred at 3.75 hours post fertilization (hpf). The overexpression of Jak2a was proven by real-time Q-PCR and Western blot in 1-4-cell stage embryos injected with 400 ng/µl full-length jak2a mRNA as well as gfi1.1, gata1, mpo and β-embryonic hemoglobin as detected by real-time Q-PCR. Moreover, jak2a mRNA significantly increased the GFP(+) population in the transgenic zebrafish lines Tg (gata1: gfp) (uninjected embryos: 17.22±1.70%; embryos injected with jak2a mRNA: 21.31±2.11%, p<0.01) and Tg (mpo: gfp) (uninjected embryos: 3.86±1.94; embryos injected with jak2a mRNA: 6.64±1.30%, p<0.01) compared with the control group. Thus, our data indicate that Jak2a plays an important role in erythropoiesis and myeloid hematopoiesis. Ivyspring International Publisher 2017-07-18 /pmc/articles/PMC5562130/ /pubmed/28824311 http://dx.doi.org/10.7150/ijms.18907 Text en © Ivyspring International Publisher This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/). See http://ivyspring.com/terms for full terms and conditions. |
spellingShingle | Research Paper Zhu, Xianmin Liu, Rui Guan, Jun Zeng, Wen Yin, Jin Zhang, Yicheng Jak2a regulates erythroid and myeloid hematopoiesis during zebrafish embryogenesis |
title | Jak2a regulates erythroid and myeloid hematopoiesis during zebrafish embryogenesis |
title_full | Jak2a regulates erythroid and myeloid hematopoiesis during zebrafish embryogenesis |
title_fullStr | Jak2a regulates erythroid and myeloid hematopoiesis during zebrafish embryogenesis |
title_full_unstemmed | Jak2a regulates erythroid and myeloid hematopoiesis during zebrafish embryogenesis |
title_short | Jak2a regulates erythroid and myeloid hematopoiesis during zebrafish embryogenesis |
title_sort | jak2a regulates erythroid and myeloid hematopoiesis during zebrafish embryogenesis |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5562130/ https://www.ncbi.nlm.nih.gov/pubmed/28824311 http://dx.doi.org/10.7150/ijms.18907 |
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