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Non-Invasive Monitoring of CNS MHC-I Molecules in Ischemic Stroke Mice

Ischemic stroke is one of the leading causes of morbidity and mortality worldwide. The expression of major histocompatibility complex class I (MHC-I) molecules in the central nervous system, which are silenced under normal physiological conditions, have been reported to be induced by injury stimulat...

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Autores principales: Xia, Jing, Zhang, Ying, Zhao, Huanhuan, Wang, Jie, Gao, Xueren, Chen, Jinpeng, Fu, Bo, Shen, Yuqing, Miao, Fengqin, Zhang, Jianqiong, Teng, Gaojun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5562219/
https://www.ncbi.nlm.nih.gov/pubmed/28824719
http://dx.doi.org/10.7150/thno.18968
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author Xia, Jing
Zhang, Ying
Zhao, Huanhuan
Wang, Jie
Gao, Xueren
Chen, Jinpeng
Fu, Bo
Shen, Yuqing
Miao, Fengqin
Zhang, Jianqiong
Teng, Gaojun
author_facet Xia, Jing
Zhang, Ying
Zhao, Huanhuan
Wang, Jie
Gao, Xueren
Chen, Jinpeng
Fu, Bo
Shen, Yuqing
Miao, Fengqin
Zhang, Jianqiong
Teng, Gaojun
author_sort Xia, Jing
collection PubMed
description Ischemic stroke is one of the leading causes of morbidity and mortality worldwide. The expression of major histocompatibility complex class I (MHC-I) molecules in the central nervous system, which are silenced under normal physiological conditions, have been reported to be induced by injury stimulation. The purpose of this study was to determine whether MHC-I molecules could serve as molecular targets for the acute phase of ischemic stroke and to assess whether a high-affinity peptide specific for MHC-I molecules could be applied in the near-infrared imaging of cerebral ischemic mice. Quantitative real-time PCR and Western blotting were used to detect the expression of MHC-I molecules in two mouse models of cerebral ischemic stroke and an in vitro model of ischemia. The NetMHC 4.0 server was used to screen a high-affinity peptide specific for mouse MHC-I molecules. The Rosetta program was used to identify the specificity and affinity of the screened peptide (histocompatibility-2 binding peptide, H2BP). The results demonstrated that MHC-I molecules could serve as molecular targets for the acute phase of ischemic stroke. Cy5.5-H2BP molecular probes could be applied in the near-infrared imaging of cerebral ischemic mice. Research on the expression of MHC-I molecules in the acute phase after ischemia and MHC-I-targeted imaging may not only be helpful for understanding the mechanism of ischemic and hypoxic brain injury and repair but also has potential application value in the imaging of ischemic stroke.
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spelling pubmed-55622192017-08-18 Non-Invasive Monitoring of CNS MHC-I Molecules in Ischemic Stroke Mice Xia, Jing Zhang, Ying Zhao, Huanhuan Wang, Jie Gao, Xueren Chen, Jinpeng Fu, Bo Shen, Yuqing Miao, Fengqin Zhang, Jianqiong Teng, Gaojun Theranostics Research Paper Ischemic stroke is one of the leading causes of morbidity and mortality worldwide. The expression of major histocompatibility complex class I (MHC-I) molecules in the central nervous system, which are silenced under normal physiological conditions, have been reported to be induced by injury stimulation. The purpose of this study was to determine whether MHC-I molecules could serve as molecular targets for the acute phase of ischemic stroke and to assess whether a high-affinity peptide specific for MHC-I molecules could be applied in the near-infrared imaging of cerebral ischemic mice. Quantitative real-time PCR and Western blotting were used to detect the expression of MHC-I molecules in two mouse models of cerebral ischemic stroke and an in vitro model of ischemia. The NetMHC 4.0 server was used to screen a high-affinity peptide specific for mouse MHC-I molecules. The Rosetta program was used to identify the specificity and affinity of the screened peptide (histocompatibility-2 binding peptide, H2BP). The results demonstrated that MHC-I molecules could serve as molecular targets for the acute phase of ischemic stroke. Cy5.5-H2BP molecular probes could be applied in the near-infrared imaging of cerebral ischemic mice. Research on the expression of MHC-I molecules in the acute phase after ischemia and MHC-I-targeted imaging may not only be helpful for understanding the mechanism of ischemic and hypoxic brain injury and repair but also has potential application value in the imaging of ischemic stroke. Ivyspring International Publisher 2017-07-08 /pmc/articles/PMC5562219/ /pubmed/28824719 http://dx.doi.org/10.7150/thno.18968 Text en © Ivyspring International Publisher This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/). See http://ivyspring.com/terms for full terms and conditions.
spellingShingle Research Paper
Xia, Jing
Zhang, Ying
Zhao, Huanhuan
Wang, Jie
Gao, Xueren
Chen, Jinpeng
Fu, Bo
Shen, Yuqing
Miao, Fengqin
Zhang, Jianqiong
Teng, Gaojun
Non-Invasive Monitoring of CNS MHC-I Molecules in Ischemic Stroke Mice
title Non-Invasive Monitoring of CNS MHC-I Molecules in Ischemic Stroke Mice
title_full Non-Invasive Monitoring of CNS MHC-I Molecules in Ischemic Stroke Mice
title_fullStr Non-Invasive Monitoring of CNS MHC-I Molecules in Ischemic Stroke Mice
title_full_unstemmed Non-Invasive Monitoring of CNS MHC-I Molecules in Ischemic Stroke Mice
title_short Non-Invasive Monitoring of CNS MHC-I Molecules in Ischemic Stroke Mice
title_sort non-invasive monitoring of cns mhc-i molecules in ischemic stroke mice
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5562219/
https://www.ncbi.nlm.nih.gov/pubmed/28824719
http://dx.doi.org/10.7150/thno.18968
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