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Computational identification and characterization of miRNAs and their target genes from five cyprinidae fishes
MicroRNAs (miRNAs) are a kind of small single-strand RNA molecules with lengths of 18–25 nt, which do not encode any proteins. They play an essential role in gene expression regulation by binding to their target genes, leading to translational repression or transcript degradation. In this study, 23...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5562384/ https://www.ncbi.nlm.nih.gov/pubmed/28855803 http://dx.doi.org/10.1016/j.sjbs.2015.05.007 |
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author | Huang, Yong Ren, Hong-Tao Zou, Quan Wang, Yu-Qin Zhang, Ji-Liang Yu, Xue-Li |
author_facet | Huang, Yong Ren, Hong-Tao Zou, Quan Wang, Yu-Qin Zhang, Ji-Liang Yu, Xue-Li |
author_sort | Huang, Yong |
collection | PubMed |
description | MicroRNAs (miRNAs) are a kind of small single-strand RNA molecules with lengths of 18–25 nt, which do not encode any proteins. They play an essential role in gene expression regulation by binding to their target genes, leading to translational repression or transcript degradation. In this study, 23 miRNAs were predicted from five cyprinidae fishes by using a bioinformatics-based gene search based on blasting ESTs and GSS in NCBI, of which 21 miRNA genes have not been previously reported. To prove their validity, five of the computationally predicted miRNAs were verified by RTPCR, their transcripts were successfully detected, and, 46 potential target genes for these miRNAs were predicted, most target genes encode transcription factors, they are involved in signal transduction, metabolism and development processes. |
format | Online Article Text |
id | pubmed-5562384 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-55623842017-08-30 Computational identification and characterization of miRNAs and their target genes from five cyprinidae fishes Huang, Yong Ren, Hong-Tao Zou, Quan Wang, Yu-Qin Zhang, Ji-Liang Yu, Xue-Li Saudi J Biol Sci Original Article MicroRNAs (miRNAs) are a kind of small single-strand RNA molecules with lengths of 18–25 nt, which do not encode any proteins. They play an essential role in gene expression regulation by binding to their target genes, leading to translational repression or transcript degradation. In this study, 23 miRNAs were predicted from five cyprinidae fishes by using a bioinformatics-based gene search based on blasting ESTs and GSS in NCBI, of which 21 miRNA genes have not been previously reported. To prove their validity, five of the computationally predicted miRNAs were verified by RTPCR, their transcripts were successfully detected, and, 46 potential target genes for these miRNAs were predicted, most target genes encode transcription factors, they are involved in signal transduction, metabolism and development processes. Elsevier 2017-09 2015-05-13 /pmc/articles/PMC5562384/ /pubmed/28855803 http://dx.doi.org/10.1016/j.sjbs.2015.05.007 Text en © 2015 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original Article Huang, Yong Ren, Hong-Tao Zou, Quan Wang, Yu-Qin Zhang, Ji-Liang Yu, Xue-Li Computational identification and characterization of miRNAs and their target genes from five cyprinidae fishes |
title | Computational identification and characterization of miRNAs and their target genes from five cyprinidae fishes |
title_full | Computational identification and characterization of miRNAs and their target genes from five cyprinidae fishes |
title_fullStr | Computational identification and characterization of miRNAs and their target genes from five cyprinidae fishes |
title_full_unstemmed | Computational identification and characterization of miRNAs and their target genes from five cyprinidae fishes |
title_short | Computational identification and characterization of miRNAs and their target genes from five cyprinidae fishes |
title_sort | computational identification and characterization of mirnas and their target genes from five cyprinidae fishes |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5562384/ https://www.ncbi.nlm.nih.gov/pubmed/28855803 http://dx.doi.org/10.1016/j.sjbs.2015.05.007 |
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